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bmp4 mrna  (Boster Bio)


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    Structured Review

    Boster Bio bmp4 mrna
    Effect of PTE on the <t>BMP4</t> <t>mRNA</t> and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.
    Bmp4 Mrna, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmp4+mrna/Swine+recombinant+BMP-4+(Bone+morphogenetic+protein-4)+protein%2C+AF/pmc06496535-48-3-14
    Average 93 stars, based on 18 article reviews
    bmp4 mrna - by Bioz Stars, 2026-09
    93/100 stars

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    1) Product Images from "Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells"

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells

    Journal: Cell Proliferation

    doi: 10.1111/j.1365-2184.2007.00431.x

    Effect of PTE on the BMP4 mRNA and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.
    Figure Legend Snippet: Effect of PTE on the BMP4 mRNA and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.

    Techniques Used: Expressing, In Vitro, Hybridization, In Situ Hybridization, Staining, Control, Comparison, Reverse Transcription, Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture

    Related Articles

    Expressing:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    In Vitro:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Hybridization:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    In Situ Hybridization:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Staining:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Control:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Comparison:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Reverse Transcription:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Polymerase Chain Reaction:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Isolation:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Cell Culture:

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.. Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).Preparation of PT extracted with ethyl acetate (PTE) Plastrum testudinis was purchased from Guangzhou Chinese Medicine Corporation (Guangzhou, China, website: http://www.gzycco.com ).

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
    Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.



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    GenScript corporation bmp4 mrna
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    Effect of PTE on the <t>BMP4</t> <t>mRNA</t> and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.
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    Effect of PTE on the BMP4 mRNA and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.

    Journal: Cell Proliferation

    Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells

    doi: 10.1111/j.1365-2184.2007.00431.x

    Figure Lengend Snippet: Effect of PTE on the BMP4 mRNA and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.

    Article Snippet: CD44, probes of BMP4 mRNA, digoxigenin‐labelled probe detection kit, and diaminobenzidine were purchased from Wuhan Boster Biological Technology Co., Ltd. (Wuhan, China); chemicals such as dimethyl sulphoxide (DMSO) and other reagents were also obtained from Sigma.

    Techniques: Expressing, In Vitro, Hybridization, In Situ Hybridization, Staining, Control, Comparison, Reverse Transcription, Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture