bmp4 mrna (Boster Bio)
Structured Review

Bmp4 Mrna, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bmp4+mrna/Swine+recombinant+BMP-4+(Bone+morphogenetic+protein-4)+protein%2C+AF/pmc06496535-48-3-14
Average 93 stars, based on 18 article reviews
Images
1) Product Images from "Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells"
Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells
Journal: Cell Proliferation
doi: 10.1111/j.1365-2184.2007.00431.x
Figure Legend Snippet: Effect of PTE on the BMP4 mRNA and BMP4 expression of MSCs in vitro. (a) Photomicrograph showing positive signal of hybridization is shown as deep brown colour within the cytoplasm in situ hybridization for BMP4 mRNA (arrow, the position where brown colour was expressed at cell cytoplasm was positive, and with haematoxylin counter stain, original magnification ×200) and BMP4‐positive cell cytoplasm were in green (white arrow, the position where green colour was expressed at cell cytoplasm was positive, the position where green colour was not expressed at cell cytoplasm was negative, and with PI counter stain, original magnification ×200), MSCs in control group showed a little BMP4 mRNA and BMP4‐positive staining, PTE treated MSCs show more strong BMP4 mRNA and BMP4‐positive staining. (b) Comparison of the percentages of BMP4 mRNA and BMP4‐positive cell among the four experimental groups after PTE treatment of 3 days. (c) Representative real‐time reverse transcription‐polymerase chain reaction demonstrating an increase in BMP4 mRNA isolated from MSC in response to a different dose of PTE (30 and 300 µg/ml culture media) for 1, 3 and 5 days. (d) Representative ELISA for BMP4 of MSCs cultured increased with increasing time and dose of PTE. *P < 0.05; **P < 0.01 compared to control.
Techniques Used: Expressing, In Vitro, Hybridization, In Situ Hybridization, Staining, Control, Comparison, Reverse Transcription, Polymerase Chain Reaction, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture
Related Articles
Expressing:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of In Vitro:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Hybridization:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of In Situ Hybridization:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Staining:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Control:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Comparison:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Reverse Transcription:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Polymerase Chain Reaction:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Isolation:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Enzyme-linked Immunosorbent Assay:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of Cell Culture:Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA); MTT, BrdUrd, and propidium iodide (PI) were purchased from Sigma (St. Louis, MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China).MO, USA); BrdUrd, PCNA, BMP4 antibody, and fluorescein isothiocyanate (FITC)‐labelled monoclonal goat antibodies were provided by Santa Cruz Biotechnologies (Santa Cruz, CA, USA); a commercially available enzyme‐linked immunosorbent assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). ... CD44, probes of Article Title: Extracts from plastrum testudinis promote proliferation of rat bone‐marrow‐derived mesenchymal stem cells Article Snippet: assay (ELISA) kit specific for rat BMP4 was purchased from Shanghai Shenergy Biocolor BioScience and Technology Co., Ltd. (Shanghai, China). .. CD44, probes of |