Journal: Nature Communications
Article Title: Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis
doi: 10.1038/s41467-022-31646-0
Figure Lengend Snippet: Debris was implanted and the calvarial bone of mice were then treated with BML111 (1 mg/kg), WRW4 (2.5 mg/kg) and Ac2–26 (1 mg/kg). a Quantification of the lytic area in calvarial bone tissues analyzed by micro-CT. Results represent the mean ± SEM of 7 mice. b , c Quantification of TRAP-stained areas and inflammatory infiltrate in calvarial bone tissues. Significant difference among the groups was determined by one-way ANOVA, followed by Tukey’s multiple-comparison procedure. * p < 0.05, ** p < 0.001, *** p < 0.0001, **** p < 0.00001. ns indicates no significant difference. d Representative images for micro-CT and histological observations of bone sections stained by TRAP and H&E. Scale bar 100 µm. Source data are provided as a Source Data file.
Article Snippet: For examining the therapeutic effects of AnxA1, eight-week-old male C57/BL6 mice was treated by N-terminal AnxA1 (Ac2–26) or BML111 (R&D Systems) was injected 5 times at a concentration of 1 mg/kg.
Techniques: Micro-CT, Staining, Comparison