Journal: bioRxiv

Article Title: Alternative Lengthening of Telomeres and CINSARC are interconnected toward non-translocation-related sarcomas progression

doi: 10.64898/2026.01.23.701253

Figure Lengend Snippet: A. The bar charts present the quantification of the % of cells presenting APBs for each cell line after 72h (left), 4 weeks (middle) and 10 weeks (right) of DOX treatment. Results are mean ± SD. BLM inhibition induced by DOX treatment was assessed in parallel by qRT-PCR (72h), qRT-PCR/western blot (4 weeks, Figs. SBA and SBB) and RNAseq (1O weeks). Unpaired t-test with Welch’s correction. *: p<0.05, ***: p<0.001 and ****: p<0.0001. B. Enrichment plots for 2 highly enriched Hallmark gene sets, for the ALT + -related and CINSARC signatures in ALT + hybrids without BLM inhibition (Ctrl, shRNA without DOX induction) compared to the same hybrids with BLM inhibition (sh+DOX, shRNA induction by DOX treatment).

Article Snippet: Real-time Quantitative RT-PCR was performed as previously described , using TaqMan TM Gene Expression assays (Applied Biosystems): Hs00172060_m1 for BLM ; Hs99999903_m1 for ACTB and Hs99999902_m1 for RPLP0 .

Techniques: Inhibition, Quantitative RT-PCR, Western Blot, shRNA

Journal: bioRxiv

Article Title: Alternative Lengthening of Telomeres and CINSARC are interconnected toward non-translocation-related sarcomas progression

doi: 10.64898/2026.01.23.701253

Figure Lengend Snippet: A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using propidium iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.

Article Snippet: Real-time Quantitative RT-PCR was performed as previously described , using TaqMan TM Gene Expression assays (Applied Biosystems): Hs00172060_m1 for BLM ; Hs99999903_m1 for ACTB and Hs99999902_m1 for RPLP0 .

Techniques: Cell Characterization, MANN-WHITNEY, Control, Inhibition, Quantitative RT-PCR, Western Blot