human bladder carcinoma cell line j82  (ATCC)

Journal: The FASEB Journal

Article Title: Intravesical Delivery of P21 mRNA –Loaded Lipid Nanoparticles as a Tumor Suppressor Replacement Therapy for Bladder Cancer

doi: 10.1096/fj.202600049R

Figure Lengend Snippet: Rationale for selecting CDKN1A /p21 as a therapeutic target in bladder cancer and validation of p21 mRNA expression. (A) Pan‐cancer analysis of CDKN1A alteration frequencies based on TCGA datasets. The analysis was performed using the TIMER3 web server. (B) CDKN1A mRNA expression in bladder cancer and normal bladder tissues, shown as log2(TPM + 1), where TPM denotes transcripts per million. Data were analyzed using the TIMER3 web server. (C) Representative p21 immunohistochemical staining images from a bladder cancer tissue microarray stratified by pathological stage (Tis, T1, T2, T3, and T4). Scale bars, 200 μm. (D) Quantification of p21 H‐scores in Tis, T1, T2, T3, and T4 lesions. Compared with Tis lesions, T1, T2, T3, and T4 tumors showed significantly lower p21 H‐scores. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01 (one‐way ANOVA). (E) Western blot analysis of basal and p21 mRNA‐induced p21 protein expression in T24 and J82 bladder cancer cells. Basal p21 protein levels were very low in both cell lines, whereas transfection of p21 mRNA led to robust p21 expression. For p21 blots, both short‐exposure (se) and long‐exposure (le) images are shown. β‐Actin serves as a loading control. (F) Immunofluorescence analysis of EGFP‐HA and p21‐HA expression in T24 and J82 cells using an anti‐HA antibody. Nuclei were stained with DAPI. The p21‐HA signal was predominantly localized to the nucleus, whereas EGFP showed diffuse cytoplasmic distribution. Scale bars, 20 μm.

Article Snippet: The human bladder carcinoma cell line J82 and the human embryonic kidney cell line HEK293T were purchased from the American Type Culture Collection (ATCC).

Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Staining, Microarray, Western Blot, Transfection, Control, Immunofluorescence

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Effect of ARAF on the proliferation of T24 cells. (A) RT-qPCR analysis of si-ARAF transfection efficiency. (B and C) Western blot analysis of si-ARAF transfection efficiency. (D) EdU fluorescence assay showing proliferating cells. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Quantitative RT-PCR, Transfection, Western Blot, Fluorescence

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Effect of ARAF on apoptosis of T24 cells. (A and B) Flow cytometry analysis of apoptosis levels. (C and D) Protein expression of cleaved-caspase 3 and total caspase 3. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. **p<0.01, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Flow Cytometry, Expressing

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Effect of ARAF on the metastatic capacity of T24 cells. (A and B) Quantification of migrating cells. (A and C) Quantification of invading cells. (D and E) Expression of EMT-related proteins in T24 cells. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Expressing

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Effect of ARAF on the p38 MAPK pathway in T24 cells. (A) The level of p-p38, p38, p-ERK, ERK, p-MEK, MEK in T24 cells was determined by western blot assay. (B) p-p38/p38 ratio. (C) p-ERK/ERK ratio. (D) p-MEK/MEK ratio. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using one-way ANOVA. ***p<0.001, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Western Blot

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Anisomycin activated p38 MAPK pathway in si-ARAF transfected T24 cells. (A) The level of p-p38, p38, p-ERK, ERK, p-MEK, MEK in T24 cells was determined by western blot assay. (B) p-p38/p38 ratio. (C) p-ERK/ERK ratio. (D) p-MEK/MEK ratio. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using Student’s t-test. ***p<0.001, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Transfection, Western Blot

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Anisomycin reversed the effect of ARAF on T24 cell proliferation and apoptosis. (A) EdU fluorescence for cell proliferation capacity. (B) The apoptotic rate of T24 cells. (C and D) The protein level of Cleaved-caspase3 and Caspase3. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using Student’s t-test. **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: Fluorescence

Journal: Open Medicine

Article Title: ARAF regulates malignant progression of bladder cancer through the p38MAPK pathway

doi: 10.1515/med-2026-1422

Figure Lengend Snippet: Anisomycin reversed the effect of ARAF on T24 cell metastasis and EMT. (A–C) The migratory and invasive ability of T24 cells. (D–F) The protein level of E-cadherin and N-cadherin. Data are presented as mean ± SD from three independent biological replicates. Statistical analysis was performed using Student’s t-test. **p<0.01, ****p<0.0001.

Article Snippet: The human ureter epithelial immortalized cell line SV-HUC-1 (CL-0222) and human bladder cancer cell lines T24 (CL-0227) and J82 (CL-0125) were purchased from Wuhan Procell Biological Company.

Techniques: