Cell Culture:Article Title: Linkage modified oligomeric compounds and uses thereof
Article Snippet: .. Immortalized human Burkitt lymphoma B cells, BJAB cells (DSMZ, Cat #ACC 757), were cultured in RPMI1640 medium containing 20% fetal bovine serum at 37° C. and 5% CO2. ..
Infection:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Article Title: Endothelial Cells Stably Infected with Recombinant Kaposi’s Sarcoma-Associated Herpesvirus Display Distinct Viscoelastic and Morphological Properties
Article Snippet: High-level illustration of sequential steps (1–6) of the approach used. (Created with BioRender.com) .. As previously described [ ], BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 primary effusion lymphoma cells, expresses the green fluorescent protein (GFP) to indicate latency and the red fluorescent protein (RFP) to indicate lytic infection driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker [ , ]. ..
Recombinant:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Article Title: Endothelial Cells Stably Infected with Recombinant Kaposi’s Sarcoma-Associated Herpesvirus Display Distinct Viscoelastic and Morphological Properties
Article Snippet: High-level illustration of sequential steps (1–6) of the approach used. (Created with BioRender.com) .. As previously described [ ], BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 primary effusion lymphoma cells, expresses the green fluorescent protein (GFP) to indicate latency and the red fluorescent protein (RFP) to indicate lytic infection driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker [ , ]. ..
Isolation:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Article Title: Endothelial Cells Stably Infected with Recombinant Kaposi’s Sarcoma-Associated Herpesvirus Display Distinct Viscoelastic and Morphological Properties
Article Snippet: High-level illustration of sequential steps (1–6) of the approach used. (Created with BioRender.com) .. As previously described [ ], BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 primary effusion lymphoma cells, expresses the green fluorescent protein (GFP) to indicate latency and the red fluorescent protein (RFP) to indicate lytic infection driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker [ , ]. ..
Marker:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Article Title: Endothelial Cells Stably Infected with Recombinant Kaposi’s Sarcoma-Associated Herpesvirus Display Distinct Viscoelastic and Morphological Properties
Article Snippet: High-level illustration of sequential steps (1–6) of the approach used. (Created with BioRender.com) .. As previously described [ ], BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 primary effusion lymphoma cells, expresses the green fluorescent protein (GFP) to indicate latency and the red fluorescent protein (RFP) to indicate lytic infection driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker [ , ]. ..
Expressing:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Virus:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
Reverse Transcription:Article Title: Endothelial cells infected with Kaposi’s sarcoma-associated herpesvirus display distinct viscoelasticity and morphology
Article Snippet: .. BJAB-rKSHV.219 were established from BJAB cells (DSMZ No.: ACC 757), a human Burkitt lymphoma-derived cell line, via stable infection with a recombinant Kaposi’s sarcoma-associated herpesvirus (rKSHV.219). rKSHV.219, isolated from JSC-1 cells, expresses the green fluorescent protein (GFP) and the red fluorescent protein (RFP) driven by cellular EF-1α and KSHV lytic PAN promoters, respectively, and contains a gene for puromycin resistance as a selectable marker., HuARLT2 cells are a conditionally-immortalised human umbilical vein endothelial cell line (HUVEC) derivative established via the expression of doxycycline-inducible transgenes simian virus 40 (SV40) large T antigen (TAg) and human telomerase reverse transcriptase (hTert). .. LECs are a telomerase-immortalised human lymphatic endothelial cell (LEC) line., Both HuARLT2 and LEC cell lines are well-established models for investigating KS mechanisms in vitro ., HEK-293T (DSMZ No.: ACC 305) cells are derived from human embryonic kidney 293 cells (HEK-293, Cellonex, C293-C) by stably expressing the transgenes SV40 and Tag.
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