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human bj 5ta fibroblast cells  (ATCC)


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    Structured Review

    ATCC human bj 5ta fibroblast cells
    Human Bj 5ta Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bj+5ta+cells/BJ-5ta/10__3390_slash_pharmaceutics18101329-65-0-4
    Average 96 stars, based on 490 article reviews
    human bj 5ta fibroblast cells - by Bioz Stars, 2026-11
    96/100 stars

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    Chromatin Immunoprecipitation:

    Article Title: RNA polymerase loss by nuclear rupture drives LMNA cardiomyopathy
    Article Snippet: The use of human autopsy specimens provided by CVPath Institute was approved by the CVPath Institute Institutional Review Board. .. We used AAVpro 293T cells (TaKaRa, 632273) for MyoAAV production and BJ-5ta cells (human hTERT-immortalized foreskin fibroblast; ATCC, CRL-4001) for ChIP-seq spike-in controls. .. Both cell lines were cultured in high-glucose DMEM (Gibco, 11965-092) supplemented with 9% fetal bovine serum (FBS) and 1:100 penicillin–streptomycin (Invitrogen, 105727) at 37°C in 5% CO 2 .

    Cell Culture:

    Article Title: Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic targets
    Article Snippet: .. BJ-5ta cells (CRL-4001, ATCC) were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS) and 1× penicillin-streptomycin at 37 °C in 5% CO 2 . .. Cas9 ribonucleoprotein (RNP) complexes were assembled using Alt-R S. pyogenes Cas9 Nuclease V3 (1081058; IDT) or corresponding nickase variants (Alt-R Cas9 D10A Nickase V3; Alt-R Cas9 H840A Nickase V3) together with Alt-R tracrRNA (1072532; IDT) and Alt-R crRNA.

    Isolation:

    Article Title: Anti-Fibrotic Properties of a Decellularized Extracellular Matrix Scaffold from Porcine Small Intestinal Submucosa in Normal Human and Keloid Fibroblasts.
    Article Snippet: .. Human keloid fibroblast cell line or, kFB (KEL FIB-CRL-1762, ATCC, VA, USA) isolated from the connective tissue of a 35-year-old black female’s skin with keloid, was grown in similar condition as the BJ-5ta cells, in Dulbecco’s Modified Eagle’s Medium (Cat# 30-2002, ATCC, VA, USA), supplemented with 10% FBS, 100 IU/mL antimicrobial-antimycotic, (Cat#15240062, ThermoFisher Scientific, Waltham, MA, USA) [54]. .. Biodesign (BioD), is an ECM-based biomaterial-derived from naturally occurring extracellular matrices of porcine small intestinal submucosa (SIS, Cook Biotech Inc., Indianapolis, IN, USA) [55,56].

    Modification:

    Article Title: Anti-Fibrotic Properties of a Decellularized Extracellular Matrix Scaffold from Porcine Small Intestinal Submucosa in Normal Human and Keloid Fibroblasts.
    Article Snippet: .. Human keloid fibroblast cell line or, kFB (KEL FIB-CRL-1762, ATCC, VA, USA) isolated from the connective tissue of a 35-year-old black female’s skin with keloid, was grown in similar condition as the BJ-5ta cells, in Dulbecco’s Modified Eagle’s Medium (Cat# 30-2002, ATCC, VA, USA), supplemented with 10% FBS, 100 IU/mL antimicrobial-antimycotic, (Cat#15240062, ThermoFisher Scientific, Waltham, MA, USA) [54]. .. Biodesign (BioD), is an ECM-based biomaterial-derived from naturally occurring extracellular matrices of porcine small intestinal submucosa (SIS, Cook Biotech Inc., Indianapolis, IN, USA) [55,56].



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    Image Search Results


    Proliferation and Immunofluorescence. (a) CCK‐8 assay, Proliferation of HDF‐Htert in the control and in the presence of TVE and HA at 24, 48, and 72 h. (b) Immunofluorescence of COL1A1, COL3A1, and ELS staining after 24 h of the treatments. Magnification: 400×. ∗ Indicates p < 0.05 compared to CTR.

    Journal: Advances in Pharmacological and Pharmaceutical Sciences

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

    doi: 10.1155/adpp/8857215

    Figure Lengend Snippet: Proliferation and Immunofluorescence. (a) CCK‐8 assay, Proliferation of HDF‐Htert in the control and in the presence of TVE and HA at 24, 48, and 72 h. (b) Immunofluorescence of COL1A1, COL3A1, and ELS staining after 24 h of the treatments. Magnification: 400×. ∗ Indicates p < 0.05 compared to CTR.

    Article Snippet: Human keratinocytes (HaCaT) (supplied by “Zooprofilattico Institute”, Brescia, Italy) and HDF cell line immortalized with hTERT (HDF cells, BJ‐5ta, ATCC CRL‐4001), were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, 1% gentamicin, and 1% amphotericin B.

    Techniques: Immunofluorescence, CCK-8 Assay, Control, Staining

    Dehydration test (a) Viability assay by MTT test. Reparative effects in HaCaT and HDF (b). Cell viability by MTT assay. ∗ p < 0.05, ∗∗ p < 0.01 compared to CTR‐ and # p < 0.05 compared to HA.

    Journal: Advances in Pharmacological and Pharmaceutical Sciences

    Article Title: Evaluation of Biological Effect of Triticum vulgare Extract Compared With Hyaluronic Acid in In Vitro Tissue Models

    doi: 10.1155/adpp/8857215

    Figure Lengend Snippet: Dehydration test (a) Viability assay by MTT test. Reparative effects in HaCaT and HDF (b). Cell viability by MTT assay. ∗ p < 0.05, ∗∗ p < 0.01 compared to CTR‐ and # p < 0.05 compared to HA.

    Article Snippet: Human keratinocytes (HaCaT) (supplied by “Zooprofilattico Institute”, Brescia, Italy) and HDF cell line immortalized with hTERT (HDF cells, BJ‐5ta, ATCC CRL‐4001), were cultured in Dulbecco’s modified Eagle medium (DMEM), supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, 1% gentamicin, and 1% amphotericin B.

    Techniques: Viability Assay, MTT Assay