bip (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc bip
Bip, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bip/BiP+Rabbit+mAb/pm42882471-83-24-25
Average 96 stars, based on 1231 article reviews

bip - by Bioz Stars, 2026-11

96/100 stars

Images

Milk:

Article Title: Proteasome Inhibition Amplifies Endoplasmic Reticulum (ER) Stress Responses: Comparative Proteomics of Chinese Hamster Ovary Cell Lines.
Article Snippet: The gels were then transferred onto a nitrocellulose membrane using the InvitrogenTM Power Blotter Select Transfer Stacks (Thermo Fischer Scientific,). .. Membranes were blocked in PierceTM Clear Milk Blocking Buffer (1×) (Thermo Fischer Scientific) for 1 h at room temperature with slight agitation and probed overnight at 4 ◦C with primary antibodies for BiP, ATF6, pan-ubiquitin, or β-actin (all antibodies, Cell Signalling Technology Europe B.V., Leiden, The Netherlands) used at 1:1000 v/v. .. Blots were then incubated with the relevant secondary antibodies (LI-COR Biosciences, UK) at 1:15,000 dilution and rinsed with 1× TBS Tween (Thermo Fischer Scientific).

Blocking Assay:

other:

Article Title: IRE1α regulates macrophage phagocytosis in immune thrombocytopenia through NR1D1 mRNA decay and lysosomal biogenesis.
Article Snippet: Data were analyzed with FlowJo Software version 10.8.1.

Cell-Signaling:

Article Title: Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF-κB axis
Article Snippet: .. BIP , 3183 , 1:1,000 , Cell Signaling Technology, Inc.. .. CHOP , 2895 , 1:1,000 , Cell Signaling Technology, Inc..

Article Title: Distinct OA:PA Ratios Generate Discrete Hepatocellular Phenotypes in HepG2 Cells.
Article Snippet: Proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA), blocked with 5% skim milk for 1 h, and incubated with primary antibodies overnight at 4 ◦C. .. Primary antibodies (all 1: 1000): AMPKα [Cell Signaling Technology (CST, Danvers, MA, USA) #2532], BAX (CST, Cat. No. 5023), BCL-2 (CST, Cat. No. 15071), BiP (CST, Cat. No. 3177S), CHOP (CST, Cat. No. 2895S), COX-2 (Abcam, Cambridge, UK, Cat. No. ab283574), FASN (CST, Cat. No. 3180S), FoxO3a (CST, Cat. No. 2497S), IL-1β (CST, https://doi.org/10.3390/cells15191774 Cat. No. 12242S), LC3A/B (CST, Cat. No. 4108S), mTOR (CST, Cat. No. 2983), NF-κB p65 (Abcam, Cat. No. ab32536), p62/SQSTM1 (CST, Cat. No. 5114S), phospho-AMPKα Thr172 (CST, Cat. No. 2535), phospho-FoxO3a Ser253 (CST, Cat. No. 9466S), phospho-mTOR Ser2448 (CST, Cat. No. 2971), phospho-NF-κB p65 Ser536 (CST, Cat. No. 3033S), SREBP-1 (CST, Cat. No. 95879S), and TNF-α (CST, Cat. No. 3707S). .. GAPDH–horseradish peroxidase (HRP) (ServiceBio, Wuhan, China, Cat. No. ZB15004-HRP; 1:5000) served as loading control for the main figure panels; recombinant anti-β-actin–HRP (ServiceBio, Cat. No. ZB15001HRP; 1:5000) served as loading control for the BAX/BCL-2 blots (Supplementary Figure S1).

Incubation:

Article Title: PRMT5-mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growth.
Article Snippet: Equal amounts of protein were separated by 12% SDS–PAGE under reducing conditions and transferred to PVDF membranes (Bio–Rad, USA). .. Membranes were blocked with TBS–T containing 5% bovine serum albumin (BSA) and incubated overnight at 4 ◦C with primary antibodies against HBsAg (Creative Diagnostics, #DMABT–51328MH), Flag (Cell Signaling Technology, #14793S), BIP (Cell Signaling Technology, #3177), β–actin (Cell Signaling Technology, #4970S), PRMT5 (Cell Signaling Technology, #2252S), monomethylarginine (IMMUNECHEM, #ICP0801), symmetric dimethyl arginine (IMMUNECHEM, #ICP0811), and asymmetric dimethyl arginine (IMMUNECHEM, #ICP0810). .. After washing, membranes were incubated with HRP–conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature.

Western Blot:

Article Title: Rational discovery of therapeutic PAK1 allosteric activators.
Article Snippet: Protein concentration was determined by Bio-Rad protein assay. .. Protein extracts (30 μg) were subject to immunoblot analyses with antibodies (all antibodies applied in this study were used as 1:1000 dilution) against PAK1 (Cell Signaling, 2602), CHOP (Cell Signaling, 2895), phospho-PAK1 (Cell signaling, 2601), phospho-PERK (Cell Signaling, 3179), ATF6 (Abcam, ab37149), BiP (Cell signaling, 3183), ATF4 (Abcam, ab85049), GADPH (Cell Signaling, 92310). ..

Review