biotinylated vicia villosa lectin (vvl, vva) (Vector Laboratories)
Vector Laboratories is a verified supplier 
Vector Laboratories manufactures this product 
95
Structured Review
Vector Laboratories
biotinylated vicia villosa lectin (vvl, vva)
Biotinylated Vicia Villosa Lectin (Vvl, Vva), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+vva/Biotinylated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/custom%40b-1235%4042414656
Average 95 stars, based on 164 article reviews
Biotinylated Vicia Villosa Lectin (Vvl, Vva), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+vva/Biotinylated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/custom%40b-1235%4042414656
Average 95 stars, based on 164 article reviews
biotinylated vicia villosa lectin (vvl, vva) - by Bioz Stars,
2026-09
95/100 stars
Images
Related Articles
Incubation:Article Title: Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsy Article Snippet: .. The samples were incubated in TBS-T for one hour with the following antibodies and lectins: Map2 (Synaptic Systems, #188011, 1:500) and Article Title: Cortical and subcortical glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons Article Snippet: PFA-fixed brains were embedded in 5 % agarose (Bioline, DM50-113D) and cut with a vibrating microtome (VT1000S, Leica Systems) into 50 μm thick coronal sections. .. Brain sections of Rbp4Cre-hM4Di and Rbp4Cre-hM3Dq mice were blocked with 2-10% normal goat or donkey serum (Sigma Aldrich) and 0.2-0.3% TritonX-100) in PBS (blocking solution, 2h at RT) and incubated with one of the following primary antibody combinations: rabbit anti-cFos (1:500, Synaptic Systems, 226-003); rabbit anti-Iba1 (1:500, FUJIFILM Wako 019-19741) and mouse anti-CD68 (1:500, Abcam, ab955); rabbit anti-Iba1 (1:500, FUJIFILM Wako 019-19741) and guinea pig anti-vGlut1 (1:500, 26 Merck Millipore AB5905); mouse anti-PSD95 (1:500, ThermoFisher 7E3-1B8) and guinea pig anti-vGlut1 (1:500, Merck Millipore AB5905); rabbit anti-GFAP (1:500, Dako Z0334) and mouse anti-S100β (1:500, Sigma-Aldrich, S2532); or rabbit anti-PV (1:500, PV27, Swant) and Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H 2 O 2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house , diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#3020-08, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Article Title: Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsy Article Snippet: 500 mg of glycoprotein lysates were precleared with 1 mL streptavidin magnetic beads (NEB #S1420S) for 1 hour at room temperature to remove endogenous biotinylated proteins as previously described. .. Glycoproteins were then incubated with the volume of 1 mL of washed/precipitated streptavidin magnetic beads (NEB #S1420S) and 100 mg Article Title: GALNT6, transcriptionally inhibited by KLF9, promotes osteosarcoma progression by increasing EFEMP1 expression via O-glycosylation modification. Article Snippet: Osteosarcoma (OS) is one of the deadliest malignancies in adolescents and its treatment status and prognosis remain unsatisfactory.. N-acetylgalactosamine transferase 6 (GALNT6), one of the key enzymes regulating O-glycosylation, functions vary in different types of cancer.. Currently, the function of GALNT6 in OS is unclear. Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma. Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H2O2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house40, diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#302008, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Blocking Assay:Article Title: Cortical and subcortical glial cells undergo rapid changes following acute chemogenetic manipulation of a subpopulation of layer 5 projection neurons Article Snippet: PFA-fixed brains were embedded in 5 % agarose (Bioline, DM50-113D) and cut with a vibrating microtome (VT1000S, Leica Systems) into 50 μm thick coronal sections. .. Brain sections of Rbp4Cre-hM4Di and Rbp4Cre-hM3Dq mice were blocked with 2-10% normal goat or donkey serum (Sigma Aldrich) and 0.2-0.3% TritonX-100) in PBS (blocking solution, 2h at RT) and incubated with one of the following primary antibody combinations: rabbit anti-cFos (1:500, Synaptic Systems, 226-003); rabbit anti-Iba1 (1:500, FUJIFILM Wako 019-19741) and mouse anti-CD68 (1:500, Abcam, ab955); rabbit anti-Iba1 (1:500, FUJIFILM Wako 019-19741) and guinea pig anti-vGlut1 (1:500, 26 Merck Millipore AB5905); mouse anti-PSD95 (1:500, ThermoFisher 7E3-1B8) and guinea pig anti-vGlut1 (1:500, Merck Millipore AB5905); rabbit anti-GFAP (1:500, Dako Z0334) and mouse anti-S100β (1:500, Sigma-Aldrich, S2532); or rabbit anti-PV (1:500, PV27, Swant) and Staining:Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H 2 O 2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house , diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#3020-08, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma. Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H2O2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house40, diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#302008, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Binding Assay:Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H 2 O 2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house , diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#3020-08, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Article Title: Differential expression of CD175 and CA19-9 in pancreatic adenocarcinoma. Article Snippet: After cooling down, sections were washed in water and treated with 0.3% H2O2 (Cat#H1009, Sigma) in deionized water for 10 min, and blocked with 10% goat serum (Cat#PCN5000, Gibco) in TBS + 0.05% Tween-20 (TBST) for 1 h. Sections were stained with a recombinant mouse anti-Tn (anti-CD175) IgM antibody (ReBaGs6, in house40, diluted to 1 μg/ml), mouse anti-SLea (CA19-9) IgG antibody (11C93, Cat#C0075-03E, VWR, diluted to 2 μg/ml), rat anti-SLex (anti-CD15s) IgM antibody (HECA-452, Cat#555,946, BD Bioscience, diluted to 2 μg/ml) or mouse anti-STn (TAG-72, B72.3) IgG antibody (Cat#sc20042, Santa Cruz Biotechnology, diluted to 2 μg/ml) in TBST overnight at 4 °C. .. After washing Sections 3 × with TBST for 10 min, horseradish peroxidase (HRP)-labeled goat anti-mouse IgM antibody (Cat#115-035-020, Jackson ImmunoResearch), or goat anti-mouse IgG antibody (Cat#115–035-003, Jackson ImmunoResearch) at 1:200 dilution in TBST were added to sections for 1 h. For CD15s staining, sections were incubated with biotinylated goat anti-rat IgM (Cat#302008, Southern Biotech, diluted at 1:200 in TBST) for 1 h, and then incubated with HRP-labeled streptavidin (Cat#SA-5014, Vector Laboratories) at 1:200 dilution in TBST for 1 h. For VVA staining, sections were incubated with Magnetic Beads:Article Title: Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsy Article Snippet: 500 mg of glycoprotein lysates were precleared with 1 mL streptavidin magnetic beads (NEB #S1420S) for 1 hour at room temperature to remove endogenous biotinylated proteins as previously described. .. Glycoproteins were then incubated with the volume of 1 mL of washed/precipitated streptavidin magnetic beads (NEB #S1420S) and 100 mg Lysis:Article Title: Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsy Article Snippet: 500 mg of glycoprotein lysates were precleared with 1 mL streptavidin magnetic beads (NEB #S1420S) for 1 hour at room temperature to remove endogenous biotinylated proteins as previously described. .. Glycoproteins were then incubated with the volume of 1 mL of washed/precipitated streptavidin magnetic beads (NEB #S1420S) and 100 mg |