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biotinylated lycopersicon esculentum tomato lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated lycopersicon esculentum tomato lectin
    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
    Biotinylated Lycopersicon Esculentum Tomato Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+lel/Biotinylated+Lycopersicon+Esculentum+(Tomato)+Lectin+(LEL%2C+TL)/pmc12893876-310-8-13
    Average 95 stars, based on 431 article reviews
    biotinylated lycopersicon esculentum tomato lectin - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation"

    Article Title: Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation

    Journal: Journal of Materials Chemistry. B

    doi: 10.1039/d5tb02179k

    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
    Figure Legend Snippet: Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Techniques Used: Staining

    Related Articles

    other:

    Article Title: Enzymatic basis of branching and extension of O-Man glycans for keratan sulfate biosynthesis.
    Article Snippet: The following antibodies and lectins were used: mouse anti-GAPDH (Merck Millipore; MAB374), rabbit anti-MGAT5B (Novus Biologicals; NBP3-05134), rabbit anti-Phosphacan (abcam; EPR26271-75), 5D4 (Merck; MABN2483), R-10G (TCI Chemicals; A2968), anti-Laminin (Sigma; L9393), HRP-antimouse IgG (GE Healthcare; NA931V), HRP-anti-human IgG (anti-Fc tag) (Southern Biotechnology Associates; 6145-05), HRP-anti-rabbit IgG (GE Healthcare; NA934V), biotinylated LEL (Vector Laboratories; B-1175-1), and L4-PHA (J-Chemical; J112).

    Article Title: Neuroprotective effects of salvianolic acids combined with Panax notoginseng saponins in cerebral ischemia/reperfusion rats concerning the neurovascular unit and trophic coupling
    Article Snippet: Types of primary antibodies: anti‐PDGFRβ at 1:200 (ab32570; Abcam), biotinylated LEL at 1:200 (B‐1175; Vector), anti‐GFAP at 1:300 (3670; CST), anti‐collagen IV at 1:400 (ab19808; Abcam), anti‐BDNF at 1:400 (ab108319; Abcam), anti‐NeuN at 1:500 (ab104224; Abcam), and anti‐TrkB at 1:100 (sc‐377218; Santa Cruz).

    Article Title: Enzymatic basis of branching and extension of O -Man glycans for keratan sulfate biosynthesis
    Article Snippet: The following antibodies and lectins were used: mouse anti-GAPDH (Merck Millipore; MAB374), rabbit anti-MGAT5B (Novus Biologicals; NBP3-05134), rabbit anti-Phosphacan (abcam; EPR26271 –75), 5D4 (Merck; MABN2483), R-10G (TCI Chemicals; A2968), anti-Laminin (Sigma; L9393), HRP-anti-mouse IgG (GE Healthcare; NA931 V), HRP-anti-human IgG (anti-Fc tag) (Southern Biotechnology Associates; 6145–05), HRP-anti-rabbit IgG (GE Healthcare; NA934 V), biotinylated LEL (Vector Laboratories; B-1175–1), and L4-PHA (J-Chemical; J112).

    Article Title: Acceleration of NLRP3 inflammasome by chronic cerebral hypoperfusion in Alzheimer's disease model mouse.
    Article Snippet: Cerebral neuroinflammation defines a novel pathway for progressing Alzheimer’s disease (AD) pathology.. We investigated immunohistological changes of neuroinflammation with nucleotide-binding domain and leucine-rich repeat (NLR)-protein 3 (NLRP3), activated caspase-1 and interleukin-1 beta (IL-1 ) in a novel AD (APP23) mice with chronic cerebral hypoperfusion (CCH) model from 4 months (M) of age, moreover, examined protective effect of galantamine.. CCH strongly enhanced NLRP3, activated caspase1 and IL-1 expressions in hippocampus and thalamus at age 12 M of AD mice.

    Article Title: Neuroprotective effects of SMTP-44D in mice stroke model in relation to neurovascular unit and trophic coupling.
    Article Snippet: The following primary antibodies were used: biotinylated LEL (1:200; Vector, B‐1175, AB_2315475); mouse anti‐NG2 antibody (1:1,000; Millipore, Burlington, MA, 05‐710, AB_309925); goat anti‐GFAP anti‐ body (1:1,000; Millipore, ab53554, AB_880202); rabbit anti‐collagen IV antibody (1:500; Abcam, ab19808, AB_445160); sheep anti‐BDNF antibody (1:100; Abcam, ab75040, AB_1280756); rabbit anti‐TrkB an‐ tibody (1:100; Santa Cruz Biotechnology, CA, sc‐8316, AB_2155274); mouse anti‐NeuN antibody (1:200; Millipore, Burlington, MA, MAB377, AB_2298772).

    Incubation:

    Article Title: Earthworm extract improves cerebral ischemia-reperfusion injury through regulating microglia polarization and promoting cerebral angiogenesis in vitro and in vivo.
    Article Snippet: Promoting the polarization of microglia towards an anti-inflammatory M2 phenotype probably be exploited as a potential strategy for stroke.. While earthworm has been shown to possess anti-inflammatory effects, its potential impact on microglial polarization and post-stroke recovery remains unclear.. In in vitro experiments, BV2 mouse microglial cells were stimulated with lipopolysaccharide (LPS).

    Article Title: Metabolic control of T cell immune response through glycans in inflammatory bowel disease
    Article Snippet: .. T cells were incubated with biotinylated L-PHA, biotinylated LEL, biotinylated SNA, or biotinylated MAL-II (Vector Labs). ..

    Expressing:

    Article Title: Effects of Pretreatment with Warfarin or Rivaroxaban on Neurovascular Unit Dissociation after Tissue Plasminogen Activator Thrombolysis in Ischemic Rat Brain.
    Article Snippet: Background: Warfarin and rivaroxaban are highly effective in reducing stroke risk in patients with atrial fibrillation (AF).. However, their effects on anticoagulation and neurovascular unit (NVU) change remain elusive.. In this study, we assessed the risks and benefits of pre-treatment with warfarin or rivaroxaban after tissuetype plasminogen activator (tPA) thrombolysis in ischemic rat brain.



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    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato <t>Lectin)</t> and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.
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    Image Search Results


    Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Journal: Journal of Materials Chemistry. B

    Article Title: Co-delivery of synaptogenic and angiogenic nanoparticles in MAP scaffolds enhances post-stroke synapse formation

    doi: 10.1039/d5tb02179k

    Figure Lengend Snippet: Inclusion of TSP-1 does not hinder the angiogenic effect of CLUVENA + MAP 15 days after stroke. (A) Experimental timeline of PT stroke surgery. (B) Schematic outlining vascular characteristics analyzed. (C) Representative 20× large image scans of the infarct and surrounding tissue. Tissues were stained for perfusable vessels (Tomato Lectin) and nuclei (DAPI). Scale bars represent 500 µm. For magnified images, scale bar represents 200 µm. (D) Vasculature density measured in the infarct normalized to void space. (E) Vessel density normalized to DAPI count in the infarct. (F) Maximum branch length measured in the infarct. DAPI positive area measured in (G) the infarct and (H) the peri-infarct. For panels (D)–(H), averaged values were plotted with error bars representing the SEM. n = 3–4. Mixed effect analysis with Sidak post hoc analysis was performed. Unadjusted p -values are indicated on the graphs.

    Article Snippet: Under anesthesia, a 50 μL retro-orbital injection of biotinylated Lycopersicon esculentum (tomato) Lectin (Vector Labs, Cat. No. B-1175-1) was administered and allowed to circulate for 10 minutes.

    Techniques: Staining