Review



vc b 1155 m005  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Vector Laboratories vc b 1155 m005
    Vc B 1155 M005, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pmc13098192-3-5-2
    Average 94 stars, based on 85 article reviews
    vc b 1155 m005 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Inositol polyphosphate multikinase regulates Th1 and Th17 cell differentiation by controlling Akt-mTOR signaling
    Article Snippet: 40 μL of each sample was transferred to a flat-bottom ELISA plate (Greiner, Kremsmunster, Austria) and incubated at 4 °C overnight. .. After washing, the plates were blocked by adding 200 μL of 0.2% I-block (Applied Biosystems) and incubating at 37 °C for 2 h. Plates were washed again, then 40 μL of biotinylated jacalin (Vector Laboratories) diluted in PBS/Tween containing 0.1% BSA (1:1000 dilution) was added and the plates were incubated at room temperature for 30 min. After a final wash, 70 μL of alkaline phosphatase substrate (5 mM p -nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and color was allowed to fully develop. ..

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation
    Article Snippet: Protein bands were further visualized using ECL reagents of Pierce (Thermo Fisher Scientific, Rockford, IL, USA). .. Cultured cells (1×10 6 ) were collected and stained with 20 μg/ml biotinylated Jacalin at 37 ̊C for 1 h. After washing with PBS, the cells were incubated with 10 μg/ml Phycoerythrin conjugated Streptavidin (SA5207; Vector Laboratories) at 4°C for 1 h in the dark. .. Then cells were resuspended in 500 μl PBS and analyzed on a FACScan flow cytometer (Beckman Coulter, Brea, CA, USA).

    Article Title: Inositol polyphosphate multikinase regulates Th1 and Th17 cell differentiation by controlling Akt-mTOR signaling.
    Article Snippet: 40 mL of each sample was transferred to a flat-bottom ELISA plate (Greiner, Kremsmunster, Austria) and incubated at 4 C overnight. .. After washing, the plates were blocked by adding 200 mL of 0.2% I-block (Applied Biosystems) and incubating at 37 C for 2 h. Plates were washed again, then 40 mL of biotinylated jacalin (Vector Laboratories) diluted in PBS/ Tween containing 0.1% BSA (1:1000 dilution) was added and the plates were incubated at room temperature for 30 min. After a final wash, 70 mL of alkaline phosphatase substrate (5 mM p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer; Sigma-Aldrich) was added and color was allowed to fully develop. ..

    Article Title: Identification of potential glycoprotein biomarkers in oral squamous cell carcinoma using sweet strategies.
    Article Snippet: The prevalence of oral squamous cell carcinoma (OSCC) is high in South and Southeast Asia regions.. Most OSCC patients are detected at advanced stages low 5-year survival rates.. Aberrant expression of glycosylated proteins was found to be associated with malignant transformation and cancer progression.

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation
    Article Snippet: .. The blotted polyvinylidene (PVDF) membranes were incubated with antibodies against C1GalT1 (ab57492; Abcam, 1:1000), GAPDH (AF1186;Beyotime,1:2000), β1-integrin(ab24693; Abcam, 1:1000), Try397 pFAK (sc81493; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:800), Ser 473 pAkt (sc293125; Santa Cruz Biotechnology, 1:800) and biotinylated Jacalin (B1115; Vector Laboratories, Burlingame, CA, 1:1000). .. The membranes were probed with HRP-conjugated anti-mouse (A0126; Beyotime, 1:2000) or anti-rabbit (A0108; Beyotime, 1:2000) secondary antibodies or Streptavidin (A0303; Beyotime, 1:2000).

    Cell Culture:

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation
    Article Snippet: Protein bands were further visualized using ECL reagents of Pierce (Thermo Fisher Scientific, Rockford, IL, USA). .. Cultured cells (1×10 6 ) were collected and stained with 20 μg/ml biotinylated Jacalin at 37 ̊C for 1 h. After washing with PBS, the cells were incubated with 10 μg/ml Phycoerythrin conjugated Streptavidin (SA5207; Vector Laboratories) at 4°C for 1 h in the dark. .. Then cells were resuspended in 500 μl PBS and analyzed on a FACScan flow cytometer (Beckman Coulter, Brea, CA, USA).

    Staining:

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation
    Article Snippet: Protein bands were further visualized using ECL reagents of Pierce (Thermo Fisher Scientific, Rockford, IL, USA). .. Cultured cells (1×10 6 ) were collected and stained with 20 μg/ml biotinylated Jacalin at 37 ̊C for 1 h. After washing with PBS, the cells were incubated with 10 μg/ml Phycoerythrin conjugated Streptavidin (SA5207; Vector Laboratories) at 4°C for 1 h in the dark. .. Then cells were resuspended in 500 μl PBS and analyzed on a FACScan flow cytometer (Beckman Coulter, Brea, CA, USA).



    Similar Products

    vc b 1155 m005  (Vector Laboratories)
    94
    Vector Laboratories vc b 1155 m005
    Vc B 1155 M005, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pmc13098192-3-5-2
    Average 94 stars, based on 1 article reviews
    vc b 1155 m005 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    jacalin biotin  (Vector Laboratories)
    94
    Vector Laboratories jacalin biotin
    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.
    Jacalin Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pmc13098192-3-0-2
    Average 94 stars, based on 1 article reviews
    jacalin biotin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    jacalin  (Vector Laboratories)
    94
    Vector Laboratories jacalin
    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/bio_rxiv__64898__2026__02__25__706656-227-35-36
    Average 94 stars, based on 1 article reviews
    jacalin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    biotinylated lectins  (Vector Laboratories)
    94
    Vector Laboratories biotinylated lectins
    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.
    Biotinylated Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pmc12904578-275-2-5
    Average 94 stars, based on 1 article reviews
    biotinylated lectins - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    anti jacalin  (Vector Laboratories)
    94
    Vector Laboratories anti jacalin
    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.
    Anti Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pmc12672989-84-46-48
    Average 94 stars, based on 1 article reviews
    anti jacalin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    histochemical staining  (Vector Laboratories)
    94
    Vector Laboratories histochemical staining
    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled <t>using</t> <t>Jacalin-biotin</t> and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.
    Histochemical Staining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+jacalin/Biotinylated+Jacalin/pm40825516-93-7-13
    Average 94 stars, based on 1 article reviews
    histochemical staining - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled using Jacalin-biotin and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.

    Journal: mSystems

    Article Title: Adaptation of Enterococcus faecalis to intestinal mucus revealed by a human colonic organoid model

    doi: 10.1128/msystems.01304-25

    Figure Lengend Snippet: Colon epithelial monolayers with histological signatures for studying Enterococcus faecalis mucosal colonization. ( A ) Schematic depicting the experimental setup. Human colon organoids grown in Matrigel were split into single cells and seeded on top of collagen-coated Transwell membranes. Once the epithelial monolayer formed, the cells were differentiated at the air-liquid interface. Transwells were used on day 7 after seeding. ( B ) Immunostaining images of differentiated organoid monolayers with MUC2 protein (yellow), nuclei stained with DAPI (blue), and mucus labeled using Jacalin-biotin and streptavidin-Cy5 (magenta). ( C ) Colony-forming units (CFU/mL) analysis of Ef growth in mucus 6 h after inoculation. Both time points represent the colony-forming units of bacteria present in the Transwell in four independent biological replicates. The horizontal black lines mark the mean. ( D ) Maximum intensity projection of Jacalin-labeled mucus (magenta) and Ef WT expressing pDasherGFP (green). ( E ) Ef WT expressing pDasherGFP (green) in the same z-plane as the Jacalin-labeled mucus (magenta) and does not colocalize with the colonoid epithelium labeled with CellMask-DeepRed (red). ( F ) Ef colony volume calculated from confocal images represented in panel C . A minimum of 148 clusters was quantified from each image of four independent biological replicates.

    Article Snippet: Jacalin-biotin , Vector Laboratories , VC-B-1155-M005.

    Techniques: Immunostaining, Staining, Labeling, Bacteria, Expressing

    Glycosyltransferase BgsB in E. faecalis is essential for growth in colonic mucus. ( A ) Table of log2FC values calculated from the Tn-seq experiment for the glycosyltransferase bgsB and a biofilm-forming factor bph . ( B ) Representative images of Ef colonies in colonic mucus at 9 h of growth from three biological replicates. Mucus was labeled using Jacalin-biotin and streptavidin-Cy5. Wild-type Ef fluorescently expressed pDasher-GFP, and bgsB and bph mutants expressed tdTomato. ( C ) Colony-forming unit (CFU/mL) quantification of the deletion mutants grown in mucus compared with the wild-type Ef after 6 h of growth. Each dot is a biological replicate (at least three independent biological replicates for each mutant), and the horizontal black lines mark the mean. ( D ) Competition between WT (green) and mutant samples (orange) with over three biological replicates, where the WT vs bgsB::Tn is marked with a filled circle and WT vs Δ bph with an unfilled triangle. Volume ratio is calculated as a fraction of the mutant signal occupying the total (mutant + WT) volume in the image. Volume expansion is calculated as a ratio to the initial volume in the respective channel. Mean and standard deviation are shown. ( E ) The same mixed samples were grown in mucus in a flow chip shown in , under the flow of 5 µL/min of minimal medium ( n = 3). Arrows indicate the same place at the start and the end of the time-lapse. Statistics in panel C were calculated using one-way ANOVA with post hoc Dunnett’s multiple comparison test (*** P < 0.001).

    Journal: mSystems

    Article Title: Adaptation of Enterococcus faecalis to intestinal mucus revealed by a human colonic organoid model

    doi: 10.1128/msystems.01304-25

    Figure Lengend Snippet: Glycosyltransferase BgsB in E. faecalis is essential for growth in colonic mucus. ( A ) Table of log2FC values calculated from the Tn-seq experiment for the glycosyltransferase bgsB and a biofilm-forming factor bph . ( B ) Representative images of Ef colonies in colonic mucus at 9 h of growth from three biological replicates. Mucus was labeled using Jacalin-biotin and streptavidin-Cy5. Wild-type Ef fluorescently expressed pDasher-GFP, and bgsB and bph mutants expressed tdTomato. ( C ) Colony-forming unit (CFU/mL) quantification of the deletion mutants grown in mucus compared with the wild-type Ef after 6 h of growth. Each dot is a biological replicate (at least three independent biological replicates for each mutant), and the horizontal black lines mark the mean. ( D ) Competition between WT (green) and mutant samples (orange) with over three biological replicates, where the WT vs bgsB::Tn is marked with a filled circle and WT vs Δ bph with an unfilled triangle. Volume ratio is calculated as a fraction of the mutant signal occupying the total (mutant + WT) volume in the image. Volume expansion is calculated as a ratio to the initial volume in the respective channel. Mean and standard deviation are shown. ( E ) The same mixed samples were grown in mucus in a flow chip shown in , under the flow of 5 µL/min of minimal medium ( n = 3). Arrows indicate the same place at the start and the end of the time-lapse. Statistics in panel C were calculated using one-way ANOVA with post hoc Dunnett’s multiple comparison test (*** P < 0.001).

    Article Snippet: Jacalin-biotin , Vector Laboratories , VC-B-1155-M005.

    Techniques: Labeling, Mutagenesis, Standard Deviation, Comparison