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Cusabio tgfbi elisa test kit

TAF15 facilitates M2-like polarization of macrophages, enhancing <t>TGFBI</t> secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) <t>ELISA</t> analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Tgfbi Elisa Test Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/beta+test+kit/Mouse+Transforming+growth+factor-beta-induced+protein+ig-h3(TGFBI)+ELISA+kit/pmc12615741-115-5-12
Average 93 stars, based on 2 article reviews

tgfbi elisa test kit - by Bioz Stars, 2026-11

93/100 stars

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1) Product Images from "TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression"

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

Journal: JHEP Reports

doi: 10.1016/j.jhepr.2025.101545

TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Figure Legend Snippet: TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Techniques Used: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay

M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Figure Legend Snippet: M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Techniques Used: In Vivo, In Vitro, Imaging, Immunofluorescence, Western Blot, Transfection, Encapsulation, Liposomes, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Derivative Assay, Immunohistochemistry, Real-time Polymerase Chain Reaction, Binding Assay

Enzyme-linked Immunosorbent Assay:

Article Title: RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation
Article Snippet: TIANamp Genomic DNA Kit , TIANGEN , Cat#DP304. .. Mouse Interferon β (IFN-β/IFNB) ELISA Kit , CUSABIO , CatE04945m. ..

Article Title: TAF15 in tumor-associated macrophages enhances pro-tumorigenic polarization and promotes cholangiocarcinoma progression
Article Snippet: .. The TGFBI ELISA test kit was purchased from 23 CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China). .. 24 Small interference transfected BMDM cells 25 Bone marrow-derived macrophages (BMDM) were isolated from tibias and femurs, 26 and they were cultured with DMEM containing 20% FBS, glutamine and 40 ng/ml 27 M-CSF (ABclonal) for 7 days before harvesting adherent BMDM.

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression
Article Snippet: .. The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China). .. Bone marrow-derived macrophages (BMDM) were isolated from tibias and femurs, and they were cultured with DMEM containing 20% FBS, glutamine, and 40 ng/ml M-CSF (ABclonal) for 7 days before harvesting adherent BMDM.

Article Title: The aldehyde dehydrogenase 2 rs671 variant enhances amyloid β pathology
Article Snippet: .. The levels of Aβ42 and Aβ40 peptides were measured using a specific human amyloid-β peptide 1−42 ELISA Kit (Cusabio, CSB-E10684h) and a human amyloid-β peptide 1−40 ELISA Kit (Cusabio, CSB-E08299h), respectively. ..

Article Title: Evaluation of male reproductive hormones of Kalahari Red and Kalawad goat bucks as affected by age and season under tropical conditions.
Article Snippet: Comprehensive understanding of reproductive hormones and environmental cues with their relationship is essential for efficient production in livestock sectors involving either assisted or natural reproductive methods.. This study evaluated the effects of age and seasonal-associated changes in the blood concentrations of testosterone and follicle stimulating hormone (FSH) of male Kalahari Red (KR) and Kalawad (KW)—crossbred goats.. Based on a 2 × 3 × 4 factorial arrangement, a total of 42 bucks (KR n = 21 and KW n = 21) were allotted into three age groups (> 1 ≤ 2 years, > 2 ≤ 3 years and > 3 ≤ 4 years) with seven KR bucks and seven KW bucks each per age group; reared under different seasons (early rainy, late rainy, early dry and late dry).

Concentration Assay:

Article Title: Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model
Article Snippet: .. Concentration of mouse NEFL (FineTest, EM1688), mouse C5b-9 (FineTest, EM1392), mouse CD5L (Boster, EK1414), human CD5L (MEIMIAN, MM-50587H1), human Aβ40 (CUSABIO, CSB-E08299h), human Aβ42 (MAI Bio, LM-2685H), and human C5b-9 (FineTest, EH3858) in plasma was assessed with commercial kits according to instructions of manufacturers. ..

Clinical Proteomics:

other:

Article Title: ILC2-derived CGRP triggers acute inflammation and nociceptive responses in bacterial cystitis.
Article Snippet: Intraperitoneal injections of CGRP (0.1 mg/kg, AnaSpec AS-60730), BIBN4096 (0.3 mg/kg, MCE HY-10095), or vehicle were administered 2 h prior to infection.

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