beads anti rea  (Miltenyi Biotec)

Journal: iScience

Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

doi: 10.1016/j.isci.2026.117639

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Article Snippet: NucleoMag NGS Clean-up and Size Select Beads , Macherey-Nagel , 744970.50.

Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

Journal: Nature Communications

Article Title: BRANCHED1 acts as a rheostat of Nuclear Factor Y-dependent epigenetic regulation in axillary bud development

doi: 10.1038/s41467-026-77029-7

Figure Lengend Snippet: Gene Ontology (GO) analysis of transcription factors (TFs) identified as upregulated ( a ) or downregulated ( b ) in wild-type (WT) seedlings treated with 2.5 μM rac -GR24 (compared to solvent control DMSO) for 7 days, assessed by RNA-seq ( n = 3 biological replicates; p value < 0.05, fold-change [FC] > 1.2). The top 10 enriched biological processes are ranked by the count of associated TFs. Color scale represents −log 10 adjusted p values determined using a one-sided hypergeometric test with Benjamini–Hochberg correction; darker red indicates higher statistical significance. Heatmaps showing expression patterns of upregulated ( c ) or downregulated ( d ) TFs in response to rac -GR24 treatment. Each column represents a replicate and each row corresponds to a gene. Color scale represents normalized Z –scores, with darker red indicating higher expression. Genes with similar expression patterns are grouped by hierarchical clustering. Representatives of upregulated TFs or downregulated FOD genes are listed. e – g FOD genes exhibit a late and sustained transcriptional response to rac -GR24 treatment. Relative expression levels of BRC1 ( e ), FUL ( f ), and CAL ( g ) were measured in WT seedlings treated with 2.5 μM rac -GR24 or DMSO (mock) for 1, 3, or 7 days. h – j SL signaling pathway is required for repressing FOD gene expression under rac -GR24 treatment. Relative expression levels of BRC1 ( h ), FUL ( i ), and CAL ( j ) were measured in WT, d14-1 , max2-3 , max3-11 , and brc1-2 seedlings treated with rac -GR24 or DMSO (mock) for 7 days. k , l Upregulation of BRC1 strongly represses FOD gene expression. Relative expression levels of FUL ( k ) and CAL ( l ) were measured in 7-day-old seedlings of WT and two independent BRC1 overexpression lines ( BRC1-OE2 and BRC1-OE12 , expressing BRC1 under the constitutive CaMV 35S promoter). For RT-qPCR assays, data are means ± s.d., with each circle representing a biological replicate ( n = 4). All P values were determined by unpaired two-tailed Student’s t -tests, with P < 0.05 considered statistically significant.

Article Snippet: RNA-seq libraries were prepared with the RNA Library Prep Kit for Illumina (NEB, E7775) and sequenced on the NovaSeq 6000 platform (Illumina) to produce paired-end 150 bp reads.

Techniques: Analysis, Solvent, Control, RNA Sequencing, Expressing, Gene Expression, Over Expression, Quantitative RT-PCR, Two Tailed Test

Journal: Nature Communications

Article Title: BRANCHED1 acts as a rheostat of Nuclear Factor Y-dependent epigenetic regulation in axillary bud development

doi: 10.1038/s41467-026-77029-7

Figure Lengend Snippet: a Schematic of Arabidopsis shoot apex decapitation showing the uppermost primary rosette (R1) branch used for length measurement and axillary tissues (shaded area) harvested for gene expression analysis. b Representative images of intact or decapitated WT and brc1-2 plants at 7 days post-decapitation. Arrowheads indicate elongated R1 branches. Scale bar, 2 cm. c Lengths of the uppermost R1 branch in WT and brc1-2 plants measured daily for 14 days post-decapitation. Data are means ± s.e.m. ( n = 10 plants). P values (for lengths at 14 days) were determined by unpaired two-tailed Student’s t -tests. d Venn diagram showing overlap of differentially expressed TFs in axillary tissues of decapitated WT and intact brc1-2 (compared to intact WT) and in seedlings of rac -GR24-treated WT (compared to DMSO-treated WT), assessed by RNA-seq ( n = 3 biological replicates; p value < 0.05, FC > 1.2). Scatterplots of expression patterns for 145 overlapping TFs showing positive correlation between intact brc1-2 and decapitated WT ( e ), and negative correlations between rac -GR24-treated WT and decapitated WT ( f ) or intact brc1-2 ( g ). Each dot represents an individual TF plotted by Log 2 FC values. Percentages in quadrants denote proportions relative to total overlapping TFs. h Heatmap with K -means clustering of 145 overlapping TFs. Box plots (left) show Z –scored expression values for TFs in each cluster ( n = 9, 76, 7, 46, and 7 TFs for C1–C5, respectively); center lines indicate medians, box bounds represent the 25th and 75th percentiles, and whiskers extend to 1.5× the interquartile range. Representative C2 and C4 TFs are listed (right). i GO analysis of C4 TFs. The top 8 enriched biological processes are ranked by the count of associated TFs. Color scale represents adjusted p values determined using a one-sided hypergeometric test with Benjamini–Hochberg correction. j – l Decapitation-induced FOD gene expression correlates with BRC1 downregulation. Relative expression levels of BRC1 ( j ), FUL ( k ), and CAL ( l ) were measured in axillary tissues of WT and brc1-2 plants at 18 h or 36 h post-decapitation. Data are means ± s.d. ( n = 5 biological replicates). P values were determined by unpaired two-tailed Student’s t -tests.

Article Snippet: RNA-seq libraries were prepared with the RNA Library Prep Kit for Illumina (NEB, E7775) and sequenced on the NovaSeq 6000 platform (Illumina) to produce paired-end 150 bp reads.

Techniques: Gene Expression, Analysis, Two Tailed Test, RNA Sequencing, Expressing