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bbsi digested px459 vector  (Addgene inc)


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    Addgene inc bbsi digested px459 vector
    Bbsi Digested Px459 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bbsi+digested+px459+vector/pSpCas9(BB)-2A-Puro+(PX459)+(Plasmid+%2348139)/pm40234049-212-15-19
    Average 96 stars, based on 1294 article reviews
    bbsi digested px459 vector - by Bioz Stars, 2026-10
    96/100 stars

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    Synthesized:

    Article Title: Lipid flippases ATP9A and ATP9B form a complex and contribute to the exocytic pathway from the Golgi.
    Article Snippet: Target sequences for the ATP9B gene were designed using the CRISPR Design Tool from the Zhang lab (https://zlab.squarespace.com/guide-design-resources). .. Complementary oligonucleotides (59-caccgcgggagccgaccggcacagc-39 and 59-aaacgctgtgccggtcggctcccgc-39; target sequences are underlined) were synthesized and introduced into the BbsI-digested PX459 vector (#48139; Addgene). .. To edit the ATP9B gene, we employed a method previously described (Tanaka et al, 2016; Nozaki et al, 2017), which involved co-transfecting the plasmid containing ATP9B target sequences and the Cas9 gene, and a donor plasmid (pDonor-tBFP-NLS-Neo; deposited in # 80766; Addgene), referred to as Donor (Figs S2 and S4).

    Plasmid Preparation:

    Article Title: Lipid flippases ATP9A and ATP9B form a complex and contribute to the exocytic pathway from the Golgi.
    Article Snippet: Target sequences for the ATP9B gene were designed using the CRISPR Design Tool from the Zhang lab (https://zlab.squarespace.com/guide-design-resources). .. Complementary oligonucleotides (59-caccgcgggagccgaccggcacagc-39 and 59-aaacgctgtgccggtcggctcccgc-39; target sequences are underlined) were synthesized and introduced into the BbsI-digested PX459 vector (#48139; Addgene). .. To edit the ATP9B gene, we employed a method previously described (Tanaka et al, 2016; Nozaki et al, 2017), which involved co-transfecting the plasmid containing ATP9B target sequences and the Cas9 gene, and a donor plasmid (pDonor-tBFP-NLS-Neo; deposited in # 80766; Addgene), referred to as Donor (Figs S2 and S4).



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    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: G3BP1 is a tunable switch that triggers phase separation to assemble stress granules

    doi: 10.1016/j.cell.2020.03.046

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Cytoscape version 3.7.1 ( Shannon et al., 2003 ) was used for the visualization of this network in . . CRISPR-Cas9-Mediated Knockout Cells gRNA for each gene was designed through http://crispr.mit.edu/ and synthesized DNA oligos were ligated into BbsI- (NEB) digested px459 vectors (Addgene, 62988) ( Ran et al., 2013 ).

    Techniques: Virus, Recombinant, Protease Inhibitor, Electron Microscopy, Lysis, Extraction, Staining, Isolation, Mutagenesis, Sequencing, Plasmid Preparation, Software, Imaging