Journal: Nature Communications

Article Title: Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer

doi: 10.1038/s41467-026-72116-1

Figure Lengend Snippet: a Volcano plot of the DEGs of bezafibrate treatment (200 µM, 16 hours) from RNA-seq analysis of CAOV3 cells (adj. P = 0.01). b Venn diagram comparison of a list of oppositely expressed genes from ascites-treated vs bezafibrate-treated cells. c , d Western blot analysis of HMGCS2 upon 2% ascites treatment from three OVCA patients ±200 µM bezafibrate (20 hours). ferroptosis sensitivity compared between CAOV3 WT and HMGCS2KO cells with ( e ) erastin or ( f ) RSL3 treatment (24 hours). g HMGCS2 expression restored in HMGCS2KO cells with hmgcs2 overexpression plasmid and ferroptosis sensitivity compared with 10 µM erastin or 125 nM RSL3 treatment (24 hours). h , i Lipid peroxidation measured via flow cytometry analysis of BODIPY TM 581/591 C11 staining in HMGCS2KO cells that were treated with 5 µM erastin (20 hours). j , k HMGCS2OE or empty vector (EV) cells treated with 10% ascites (16 hours) and lipid droplet levels measured via flow cytometry analysis of BODIPY TM 493/503 staining. l Cell viability assessed via treatment with 10 µM erastin ±20 µM baicalin with 2% ascites (24 hours). m , n Cells treated with 10% ascites ±25 µM baicalin (24 hours) and lipid droplet levels measured as described. o Cell viability assessed in cells treated with 10 µM erastin ±100 µM malonyl CoA lithium (24 hours). p , q Cells treated with 100 µM malonyl CoA lithium (16 hours) and lipid droplet levels ( q ) measured as described. r Patient survival data extracted from TCGA and analyzed via the GEPIA 2 analysis tool ( n in panel). Data represent biologically independent replicates. Unless indicated otherwise, n = 3. Data represent mean ±s.d. where applicable. Statistical significance assessed using Wald test ( a ), one-way ANOVA ( d , g – o), t -test ( e, f , and q ), and log-rank (Mantel-Cox) test ( r ). Multiple comparisons adjusted using Holm-Šídák’s. P values for a adjusted using Benjamini-Hochberg correction. Statistical tests were two-tailed where applicable. Source data, including uncropped blots, are provided as a Source Data file.

Article Snippet: Baicalin (HY-N0197) and malonyl CoA lithium (HY-136408) were purchased from MedChemExpress.

Techniques: RNA Sequencing, Comparison, Western Blot, Expressing, Over Expression, Plasmid Preparation, Flow Cytometry, Staining, Two Tailed Test

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: BT's Artificial Intelligence (AI)-driven Design, Theoretical Verification, and Self-assembly Strategy. a , Flowchart of the AI-assisted screening process for identifying the optimal molecular partner for baicalin (BA). b , Confusion matrix of the AI model on the validation set. c , Physical image of baicalin (top). Chemical structure and physical image of tranexamic acid (TA) (bottom). d , Electrostatic potential (ESP) surface plot of the BA-TA (BT) assembly. Red and blue areas represent positive and negative electrostatic potential, respectively. e , Visualization of the interaction region indicator (IRI) analysis between BT. f, g , Scatter plots of the IRI interaction analysis and corresponding color bars, where sign(λ 2 )ρ is the product of the electron density and the second Hessian eigenvalue. h , Conceptual schematic of the BT supramolecular assembly formed by multipoint hydrogen bonding between BA and TA.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Biomarker Discovery

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Preparation, Characterization, and Performance Analysis of the baicalin-tranexamic acid (BT) Supramolecular Assembly . a , 1 H NMR spectra of baicalin (BA), tranexamic acid (TA), and the BT assembly. b , Local magnification of the spectrum in (a). c , The 2D NOESY spectrum of the BT assembly. d , Fourier-transform infrared spectroscopy (FTIR) spectra of BA, TA, and the BT assembly. e , Differential scanning calorimetry (DSC) curves of BA, TA, and the BT assembly. f , Thermogravimetric analysis (TGA) curves of BA, TA, and the BT assembly. g , Scanning electron microscopy (SEM) images of the solid-state powders of BA, TA, B + T, and the BT assembly. Scale bar = 10 μm h , Transmission electron microscopy (TEM) image of nanoparticles formed by the self-assembly of the BT assembly in water. Scale bar = 500 nm. i , Comparison of the apparent aqueous solubility of BA as a raw drug versus within the BT assembly.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Differential Scanning Calorimetry, Electron Microscopy, Transmission Assay, Comparison, Solubility

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Construction and Characterization of the Supramolecular Hybrid Nanoplatform (DHBTC). a , Schematic illustration of the preparation of DHBTC. b, c , Electrostatic potential (ESP) and simulated structure diagram of DHBTC complex. d, e , Interaction region indicator (IRI) analysis and corresponding scatter plot for the DHBTC complex. f , Scanning electron microscopy (SEM) images of HP-β-CD, the baicalin-tranexamic acid (BT) assembly, and the final DHBTC product. Scale bar = 10 μm g , Particle size distributions of BT and DHBTC. h , Comparison of the apparent aqueous solubility of baicalin (BA) as a raw drug within DHBTC. i, j , Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) curves of HP-β-CD, BT, and DHBTC.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Electron Microscopy, Comparison, Solubility, Differential Scanning Calorimetry

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Transdermal Delivery Performance and Mechanism of DHBTC. a , Schematic diagram of the in vitro permeability evaluation of DHBTC. b , Molecular dynamics (MD) simulation showing the passage of baicalin (BA) and BA-tranexamic acid (BT) through the simulated skin lipid bilayer. c , Confocal laser scanning microscopy (CLSM) images of ex vivo porcine skin from different treatment groups after Franz diffusion cell experiments (BA was labeled with FITC). Scale bar = 1000 μm d , Semi-quantitative analysis of fluorescence intensity in (c). e , Cumulative permeation of BA in different treatment groups after 20 h f , Retention of BA in different skin layers after in vitro permeation experiments. g , FTIR spectra of detached skin stratum corneum before and after in vitro permeation experiment. Results are shown as mean ± SD, ns = no significant difference, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: In Vitro, Permeability, Confocal Laser Scanning Microscopy, Ex Vivo, Diffusion-based Assay, Labeling, Fluorescence