Journal: Frontiers in Pharmacology

Article Title: Gut microbiota-derived butyrate contributes to baicalin-induced attenuation of hypertensive vascular remodeling via adventitial immunity

doi: 10.3389/fphar.2026.1835174

Figure Lengend Snippet: Baicalin attenuates Ang II-induced hypertension and restores systemic intestinal barrier integrity via a microbiota-dependent mechanism. (A) Longitudinal monitoring of systolic blood pressure (SBP) over 28 days via tail-cuff plethysmography. Mice were administered Baicalin (oral), Baicalein (i.p.), or Baicalin + Antibiotics alongside Ang II infusion. (B,C) Assessment of in vivo systemic intestinal permeability. Serum fluorescence concentrations were measured 4 h after oral gavage of 4 kDa FITC-dextran (B) or 70 kDa FITC-dextran (C) . (D) Representative Hematoxylin and Eosin (H&E) staining of colonic sections. Scale bars: 50 μm (left) and 20 μm (right). (E) Histological quantification of the ratio of intestinal crypt depth to mucosal area. (F) Quantification of plasma lipopolysaccharide (LPS) levels by ELISA. (G) Quantification of free LPS concentrations within the cecal contents. Data are presented as mean ± SEM (n = 7 per group). For longitudinal SBP data in (A) , repeated-measures two-way ANOVA was used to evaluate time, treatment, and time-by-treatment interaction effects. For endpoint measurements in (B,C,E–G) , one-way ANOVA followed by Tukey’s post hoc test was used. Brackets indicate the specific pairwise comparisons shown in each panel. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n.s., not significant.

Article Snippet: MedChemExpress supplied Baicalin (≥98% purity) and Baicalein.

Techniques: In Vivo, Permeability, Fluorescence, Staining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: BT's Artificial Intelligence (AI)-driven Design, Theoretical Verification, and Self-assembly Strategy. a , Flowchart of the AI-assisted screening process for identifying the optimal molecular partner for baicalin (BA). b , Confusion matrix of the AI model on the validation set. c , Physical image of baicalin (top). Chemical structure and physical image of tranexamic acid (TA) (bottom). d , Electrostatic potential (ESP) surface plot of the BA-TA (BT) assembly. Red and blue areas represent positive and negative electrostatic potential, respectively. e , Visualization of the interaction region indicator (IRI) analysis between BT. f, g , Scatter plots of the IRI interaction analysis and corresponding color bars, where sign(λ 2 )ρ is the product of the electron density and the second Hessian eigenvalue. h , Conceptual schematic of the BT supramolecular assembly formed by multipoint hydrogen bonding between BA and TA.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Biomarker Discovery

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Preparation, Characterization, and Performance Analysis of the baicalin-tranexamic acid (BT) Supramolecular Assembly . a , 1 H NMR spectra of baicalin (BA), tranexamic acid (TA), and the BT assembly. b , Local magnification of the spectrum in (a). c , The 2D NOESY spectrum of the BT assembly. d , Fourier-transform infrared spectroscopy (FTIR) spectra of BA, TA, and the BT assembly. e , Differential scanning calorimetry (DSC) curves of BA, TA, and the BT assembly. f , Thermogravimetric analysis (TGA) curves of BA, TA, and the BT assembly. g , Scanning electron microscopy (SEM) images of the solid-state powders of BA, TA, B + T, and the BT assembly. Scale bar = 10 μm h , Transmission electron microscopy (TEM) image of nanoparticles formed by the self-assembly of the BT assembly in water. Scale bar = 500 nm. i , Comparison of the apparent aqueous solubility of BA as a raw drug versus within the BT assembly.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Differential Scanning Calorimetry, Electron Microscopy, Transmission Assay, Comparison, Solubility

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Construction and Characterization of the Supramolecular Hybrid Nanoplatform (DHBTC). a , Schematic illustration of the preparation of DHBTC. b, c , Electrostatic potential (ESP) and simulated structure diagram of DHBTC complex. d, e , Interaction region indicator (IRI) analysis and corresponding scatter plot for the DHBTC complex. f , Scanning electron microscopy (SEM) images of HP-β-CD, the baicalin-tranexamic acid (BT) assembly, and the final DHBTC product. Scale bar = 10 μm g , Particle size distributions of BT and DHBTC. h , Comparison of the apparent aqueous solubility of baicalin (BA) as a raw drug within DHBTC. i, j , Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) curves of HP-β-CD, BT, and DHBTC.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Electron Microscopy, Comparison, Solubility, Differential Scanning Calorimetry

Journal: Bioactive Materials

Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program

doi: 10.1016/j.bioactmat.2026.01.027

Figure Lengend Snippet: Transdermal Delivery Performance and Mechanism of DHBTC. a , Schematic diagram of the in vitro permeability evaluation of DHBTC. b , Molecular dynamics (MD) simulation showing the passage of baicalin (BA) and BA-tranexamic acid (BT) through the simulated skin lipid bilayer. c , Confocal laser scanning microscopy (CLSM) images of ex vivo porcine skin from different treatment groups after Franz diffusion cell experiments (BA was labeled with FITC). Scale bar = 1000 μm d , Semi-quantitative analysis of fluorescence intensity in (c). e , Cumulative permeation of BA in different treatment groups after 20 h f , Retention of BA in different skin layers after in vitro permeation experiments. g , FTIR spectra of detached skin stratum corneum before and after in vitro permeation experiment. Results are shown as mean ± SD, ns = no significant difference, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Baicalin (BA, purity ≥98 %) and Tranexamic Acid (TA, purity ≥99 %) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: In Vitro, Permeability, Confocal Laser Scanning Microscopy, Ex Vivo, Diffusion-based Assay, Labeling, Fluorescence