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bacteriophage pp7  (ATCC)


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    Structured Review

    ATCC bacteriophage pp7
    Bacteriophage Pp7, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacteriophage+pp7/Pseudomonas+aeruginosa+bacteriophage+PP7/pm41619117-76-1-21
    Average 94 stars, based on 22 article reviews
    bacteriophage pp7 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Control:

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype.
    Article Snippet: Water Viral Concentration Method Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype
    Article Snippet: Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa ® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Article Title: Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage.
    Article Snippet: Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters.. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes.. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants.

    Produced:

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype.
    Article Snippet: Water Viral Concentration Method Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Article Title: Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.
    Article Snippet: • Increase in skimmed-milk concentration improved adenovirus recovery rate

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype
    Article Snippet: Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa ® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Incubation:

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype.
    Article Snippet: Water Viral Concentration Method Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Article Title: Rotavirus Outbreak Traced in Clinical, Environmental, and Food Matrices: Whole Genomic Characterization of an Equine-like G3P[8] Genotype
    Article Snippet: Viral particles from water samples were concentrated using the adsorption-elution method with positively charged membranes, followed by ultrafiltration through Amicon Ultra 50 kDa ® filters (Millipore, Tokyo, Japan), in accordance with ISO 15216-1:2017 standards for water samples. .. The bacteriophage PP7, kindly provided by Dr. Verônica Rajal, was used as internal control process and produced as following: Pseudomonas aeruginosa (ATCC 15692-B2) was grown on nutrient agar at 37 °C for 18–24 h. A loopful of colonies was inoculated into 10 mL nutrient broth (8 g/L) and incubated overnight at 37 °C. ..

    Isolation:

    Article Title: Comparative enteric viruses and coliphage removal during wastewater treatment processes in a sub-tropical environment.
    Article Snippet: • Enteric virus removal efficacy of wastewater treatment plants with activated sludge (ASP) process was determined.. • ASP in sub-tropical climate could be an effective treatment barrier with N3 log10 removal of enteric virus.. • Adenovirus was more resistant to removal compared to polyomavirus and torque teno virus.

    Amplification:

    Article Title: Comparative enteric viruses and coliphage removal during wastewater treatment processes in a sub-tropical environment.
    Article Snippet: • Enteric virus removal efficacy of wastewater treatment plants with activated sludge (ASP) process was determined.. • ASP in sub-tropical climate could be an effective treatment barrier with N3 log10 removal of enteric virus.. • Adenovirus was more resistant to removal compared to polyomavirus and torque teno virus.

    Polymerase Chain Reaction:

    Article Title: Comparative enteric viruses and coliphage removal during wastewater treatment processes in a sub-tropical environment.
    Article Snippet: • Enteric virus removal efficacy of wastewater treatment plants with activated sludge (ASP) process was determined.. • ASP in sub-tropical climate could be an effective treatment barrier with N3 log10 removal of enteric virus.. • Adenovirus was more resistant to removal compared to polyomavirus and torque teno virus.

    Purification:

    Article Title: Comparative enteric viruses and coliphage removal during wastewater treatment processes in a sub-tropical environment.
    Article Snippet: • Enteric virus removal efficacy of wastewater treatment plants with activated sludge (ASP) process was determined.. • ASP in sub-tropical climate could be an effective treatment barrier with N3 log10 removal of enteric virus.. • Adenovirus was more resistant to removal compared to polyomavirus and torque teno virus.

    Cloning:

    Article Title: Comparative enteric viruses and coliphage removal during wastewater treatment processes in a sub-tropical environment.
    Article Snippet: • Enteric virus removal efficacy of wastewater treatment plants with activated sludge (ASP) process was determined.. • ASP in sub-tropical climate could be an effective treatment barrier with N3 log10 removal of enteric virus.. • Adenovirus was more resistant to removal compared to polyomavirus and torque teno virus.

    Concentration Assay:

    Article Title: Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage.
    Article Snippet: Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters.. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes.. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants.

    Recombinant:

    Article Title: Viral tools for detection of fecal contamination and microbial source tracking in wastewater from food industries and domestic sewage.
    Article Snippet: Alternative indicators may be more suitable than thermotolerant coliform bacteria to assess enteric virus pollution in environmental waters and their removal from wastewaters.. In this study, F-specific RNA bacteriophages (F-RNAPh) showed to be potential viral indicators of fecal contamination when they were quantified from domestic and food-industrial effluents containing human, chicken, swine or bovine wastes.. In addition, they showed to be resistant to the primary and secondary treatments of the wastewater treatment plants.



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    pH modulates the affinity of B19V for globoside and VP1uR. ( A ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C for 1 h or at 37 °C for 30 min under neutral (pH 7.2) or acidic (pH 5.0) conditions. Cells were subsequently washed to remove unbound virus and fixed and stained for VP1u using an anti-Flag antibody. Where indicated, a trypsinization (tryp) step was performed to remove bound VP1u from the cell surface. ( B ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C and neutral pH for 30 min followed by acidification (pH 5.0) and incubation for another 30 min. Cells were washed with ice-cold buffer at pH 5.0, fixed, and stained for VP1u using an anti-Flag antibody. ( C ) Binding affinity of B19V for globoside was determined by incubating purified B19V (3 × 10 9 ) with RBCs (0.5%) in 100 µL buffer at pH ranging from 7.2 to 5.6 at RT for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. The binding affinity of VP1u for VP1uR was quantified by incubating <t>PP7-VP1u</t> (10 10 ) with UT7/Epo cells in 100 µL buffer at pH ranging from 7.2 to 5.6 at 4 °C for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. Results are presented as the mean of two independent experiments ± standard deviation (SD).
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    Image Search Results


    pH modulates the affinity of B19V for globoside and VP1uR. ( A ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C for 1 h or at 37 °C for 30 min under neutral (pH 7.2) or acidic (pH 5.0) conditions. Cells were subsequently washed to remove unbound virus and fixed and stained for VP1u using an anti-Flag antibody. Where indicated, a trypsinization (tryp) step was performed to remove bound VP1u from the cell surface. ( B ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C and neutral pH for 30 min followed by acidification (pH 5.0) and incubation for another 30 min. Cells were washed with ice-cold buffer at pH 5.0, fixed, and stained for VP1u using an anti-Flag antibody. ( C ) Binding affinity of B19V for globoside was determined by incubating purified B19V (3 × 10 9 ) with RBCs (0.5%) in 100 µL buffer at pH ranging from 7.2 to 5.6 at RT for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. The binding affinity of VP1u for VP1uR was quantified by incubating PP7-VP1u (10 10 ) with UT7/Epo cells in 100 µL buffer at pH ranging from 7.2 to 5.6 at 4 °C for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. Results are presented as the mean of two independent experiments ± standard deviation (SD).

    Journal: Cells

    Article Title: Globoside Is an Essential Intracellular Factor Required for Parvovirus B19 Endosomal Escape

    doi: 10.3390/cells13151254

    Figure Lengend Snippet: pH modulates the affinity of B19V for globoside and VP1uR. ( A ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C for 1 h or at 37 °C for 30 min under neutral (pH 7.2) or acidic (pH 5.0) conditions. Cells were subsequently washed to remove unbound virus and fixed and stained for VP1u using an anti-Flag antibody. Where indicated, a trypsinization (tryp) step was performed to remove bound VP1u from the cell surface. ( B ) Recombinant VP1u was allowed to interact with UT7/Epo cells at 4 °C and neutral pH for 30 min followed by acidification (pH 5.0) and incubation for another 30 min. Cells were washed with ice-cold buffer at pH 5.0, fixed, and stained for VP1u using an anti-Flag antibody. ( C ) Binding affinity of B19V for globoside was determined by incubating purified B19V (3 × 10 9 ) with RBCs (0.5%) in 100 µL buffer at pH ranging from 7.2 to 5.6 at RT for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. The binding affinity of VP1u for VP1uR was quantified by incubating PP7-VP1u (10 10 ) with UT7/Epo cells in 100 µL buffer at pH ranging from 7.2 to 5.6 at 4 °C for 1 h. Cells were washed with incubation buffer and DNA was extracted and quantified by qPCR. Results are presented as the mean of two independent experiments ± standard deviation (SD).

    Article Snippet: PP7 bacteriophages were obtained from ATCC, propagated in Pseudomonas aeruginosa , and modified as previously indicated [ ].

    Techniques: Recombinant, Virus, Staining, Incubation, Binding Assay, Purification, Standard Deviation