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bacteriophages phix174  (DSMZ)


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    DSMZ bacteriophages phix174
    Bacteriophages Phix174, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacteriophage+ms2/Escherichia+phage+MS2/10__1016_slash_j__jece__2026__125253-58-1-40
    Average 94 stars, based on 55 article reviews
    bacteriophages phix174 - by Bioz Stars, 2026-11
    94/100 stars

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    Infection:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Incubation:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Derivative Assay:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Titration:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Control:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Antiviral Assay:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Standard Deviation:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Virus:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Concentration Assay:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.

    Activity Assay:

    Article Title: Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal
    Article Snippet: The bacteriophage MS2 (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ] 13,767 strain) was propagated using Escherichia coli ( E. coli ) (DSMZ 5,695 strain) as the host organism.. E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.E. coli was cultured overnight at 37 °C with shaking at 220 revolutions per minute (rpm) in 10 mL of autoclaved American Type Culture Collection (ATCC) medium 271, composed of 10 g/L tryptone, 1 g/L yeast extract, 8 g/L sodium chloride, 1 g/L glucose, and 0.3 g/L calcium chloride.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities.
    Article Snippet: 2.9.2.. Using Non-Enveloped Bacteriophage MS2 E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 ◦C at a speed of 240 rpm.

    Article Title: Biocompatible Alginate Hydrogel Film Containing Acetic Acid Manifests Broad-Spectrum Antiviral and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.The bacteria were grown on a TSA plate and then in liquid TSB at 37 °C at a speed of 240 rpm.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses.
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gramnegative Pseudomonas syringae and E. coli, respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Chitosan Films Containing Acetic Acid Manifested Potent Antiviral Activity against Enveloped and Non-Enveloped Viruses
    Article Snippet: Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.Enveloped bacteriophage phi6 and non-enveloped bacteriophage MS2 infect the Gram-negative Pseudomonas syringae and E. coli , respectively.. The Pseudomonas syringae (DSM 21482), the enveloped bacteriophage phi6 (DSM 21518), E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.P. syringae and E. coli were first grown on a TSA plate and then in liquid TSB.

    Article Title: Biocompatible Alginate Film Crosslinked with Ca 2+ and Zn 2+ Possesses Antibacterial, Antiviral, and Anticancer Activities
    Article Snippet: E. coli (DSM 5695) and bacteriophage MS2 (DSM 13767) were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).. E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.E. coli was grown in TSA and subsequently in TSB at 37 °C and a speed of 240 rpm.

    Article Title: Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event.
    Article Snippet: Bacteriophage MS2 (DSMZ 13767) and MS2 host strain Escherichia coli (DSMZ 5695) were obtained from the DSMZ (German Collection of Microorganisms and Cell Cultures GmbH).. Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.Isolation and purification of MS2 phages after propagation with a co-incubation of E. coli 5695 were performed following the protocol described in Feichtmayer (2019) to produce a high-density phage suspension in buffer solution.



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    ATCC escherichia coli

    Escherichia Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacteriophage+ms2/Escherichia+coli+bacteriophage+MS2/10__1016_slash_j__watres__2026__126139-61-1-3
    Average 97 stars, based on 1 article reviews
    escherichia coli - by Bioz Stars, 2026-11
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    Inactivation of (A) MS2 bacteriophage under UV254 and (B) MS2 bacteriophage under UV222 (C) T1UV bacteriophage under UV222 at varying nitrate concentrations: 0, 4, and 8 mg-N/L (legend refers to nitrate concentration) in buffered lab water. Log reduction value (LRV) was calculated using </xref> , modeled fitted using </xref> . Error bars represent one standard deviation of the means ( n = 3). All UV doses were calculated using average irradiance which includes the water factor, reflectance factor, Petri factor and divergence factor.

    Journal: ACS Es&t Water

    Article Title: Role of Nitrate-Driven Radical Formation in Microorganism Inactivation under 222 nm UV Irradiation

    doi: 10.1021/acsestwater.5c01533

    Figure Lengend Snippet: Inactivation of (A) MS2 bacteriophage under UV254 and (B) MS2 bacteriophage under UV222 (C) T1UV bacteriophage under UV222 at varying nitrate concentrations: 0, 4, and 8 mg-N/L (legend refers to nitrate concentration) in buffered lab water. Log reduction value (LRV) was calculated using , modeled fitted using . Error bars represent one standard deviation of the means ( n = 3). All UV doses were calculated using average irradiance which includes the water factor, reflectance factor, Petri factor and divergence factor.

    Article Snippet: MS2 bacteriophages (ATCC 15597-B1) were propagated and quantified using E. coli F amp (ATCC 700891) as the host.

    Techniques: Concentration Assay, Standard Deviation

    MS2 inactivation rate constant at different initial nitrate, uric acid and TBA concentrations (table refers to the concentrations). Error bars represent standard deviation of duplicate experiments ( n = 2).

    Journal: ACS Es&t Water

    Article Title: Role of Nitrate-Driven Radical Formation in Microorganism Inactivation under 222 nm UV Irradiation

    doi: 10.1021/acsestwater.5c01533

    Figure Lengend Snippet: MS2 inactivation rate constant at different initial nitrate, uric acid and TBA concentrations (table refers to the concentrations). Error bars represent standard deviation of duplicate experiments ( n = 2).

    Article Snippet: MS2 bacteriophages (ATCC 15597-B1) were propagated and quantified using E. coli F amp (ATCC 700891) as the host.

    Techniques: Standard Deviation

    Journal: medRxiv

    Article Title: Converting Passive Filtration Media into Active Air Biofiltration Surfaces for Airborne Viral Reduction

    doi: 10.64898/2026.04.29.26352113

    Figure Lengend Snippet:

    Article Snippet: Aerosol challenge experiments were conducted at Nelson Laboratories using MS2 bacteriophage as the challenge organism under respiratory-relevant conditions.

    Techniques: Filtration