anti-tgf-β1/tgfb1 antibody ba0290  (Boster Bio)

Journal: Oncology Letters

Article Title: Simultaneous blastic plasmacytoid dendritic cell neoplasm and myelofibrosis: A case report

doi: 10.3892/ol.2024.14354

Figure Lengend Snippet: Reagents for immunohistochemistry.

Article Snippet: Anti-TGF-β1/TGFB1 Antibody , Boster Biological Technology , BA0290.

Techniques: Immunohistochemistry, Staining, Paraffin Wax

Journal: Experimental and Therapeutic Medicine

Article Title: Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro

doi: 10.3892/etm.2018.6909

Figure Lengend Snippet: Administration of Rhy exerted an anti-asthma function by suppressing the TGF-β1-mediated Smad and MAPK signaling in vivo . Allergic asthma was induced by ovalbumin administration in mice. Subsequently, lung tissues were collected and subjected to western blotting in order to determine the expression levels of molecules involved in TGF-β1-induced Smad and MAPK signaling. Six replicates of each assay were performed, and data are presented as the mean ± standard deviation. *P<0.05 vs. sham group; # P<0.05 vs. asthma group. Rhy, rhynchophylline; TGF-β1, transforming growth factor-β1; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase.

Article Snippet: An antibody against TGF-β1 (cat. no. BA0290) was purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: In Vivo, Western Blot, Expressing, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro

doi: 10.3892/etm.2018.6909

Figure Lengend Snippet: Administration of Rhy attenuated TGF-β1-induced hyperplasia of airway smooth muscle cells. The cells were incubated with 5 ng/ml TGF-β1 alone, or in combination with 10 µM Rhy or 10 µM SB431542 for 24 h. (A) Cell viability was detected with an MTT assay. (B) PCNA-positive cells and (C) immunofluorescence staining images are shown. Each assay was conducted in triplicate, and data are presented as the mean ± standard deviation. Magnification, ×400. Scale bar, 200 µm. Rhy, rhynchophylline; TGF-β1, transforming growth factor-β1; PCNA, proliferating cell nuclear antigen; DAPI, 4′,6-diamino-2-phenylindole. *P<0.05 vs Blank group. # P<0.05 vs. TGF-β1 group.

Article Snippet: An antibody against TGF-β1 (cat. no. BA0290) was purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Incubation, MTT Assay, Immunofluorescence, Staining, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor-β1-mediated Smad and mitogen-activated protein kinase signaling transductions in vivo and in vitro

doi: 10.3892/etm.2018.6909

Figure Lengend Snippet: Administration of Rhy suppressed TGF-β1-mediated Smad and MAPK signaling in airway smooth muscle cells. The cells were incubated with 5 ng/ml TGF-β1 alone, or in combination with 10 µM Rhy or 10 µM SB431542 for 24 h. Western blotting was performed to determine the expression levels of molecules involved in TGF-β1-mediated Smad and MAPK signaling. Each assay was conducted in triplicate, and data are presented as the mean ± standard deviation. *P<0.05 vs. blank group; # P<0.05 vs. TGF-β1 group. Rhy, rhynchophylline; TGF-β1, transforming growth factor-β1; MAPK, mitogen-activated protein kinase; ERK, extracellular signal-regulated kinase.

Article Snippet: An antibody against TGF-β1 (cat. no. BA0290) was purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Incubation, Western Blot, Expressing, Standard Deviation

Journal: Scientific Reports

Article Title: Calcium sulfate induced versus PMMA-induced membrane in a critical-sized femoral defect in a rat model

doi: 10.1038/s41598-017-17430-x

Figure Lengend Snippet: Western-blot analysis revealed the presence of TGF-β1 and BMP-2 in the induced membranes harvested from CS and PMMA groups at 2, 4 and 6 weeks. The TGF-β1 and BMP-2 protein contents went up gradually at 2, 4 and 6 weeks but declined after 6 weeks.

Article Snippet: TGF-β1 and BMP-2 were detected using monoclonal goat anti-rabbit TGF-β1 antibody (BA0290, Boster) and monoclonal goat anti-mouse BMP-2 (Ab6285, Abcam), respectively.

Techniques: Western Blot

Journal: Scientific Reports

Article Title: Calcium sulfate induced versus PMMA-induced membrane in a critical-sized femoral defect in a rat model

doi: 10.1038/s41598-017-17430-x

Figure Lengend Snippet: The expressions of VEGF, TGF-β1 and BMP-2 were insignificantly higher in CS-induced membranes than in PMMA-induced ones at 2, 4, 6 and 8 weeks ( A , B , C ). The expression of IL-6 showed a gradually decreasing tendency in the two experimental groups; it was significantly higher in PMMA-induced membranes than in CS-induced ones at the 2nd week but insignificantly higher at 4, 6 and 8 weeks ( D ).

Article Snippet: TGF-β1 and BMP-2 were detected using monoclonal goat anti-rabbit TGF-β1 antibody (BA0290, Boster) and monoclonal goat anti-mouse BMP-2 (Ab6285, Abcam), respectively.

Techniques: Expressing