Review



atomic force microscope multimode spm  (Veeco)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Veeco atomic force microscope multimode spm
    Atomic Force Microscope Multimode Spm, supplied by Veeco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+afm/pmc12207278-65-9-11
    Average 90 stars, based on 1 article reviews
    atomic force microscope multimode spm - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Low Pressure Radio-Frequency Oxygen Plasma Induced Oxidation of Titanium – Surface Characteristics and Biological Effects
    Article Snippet: .. An atomic force microscope (MultiMode SPM, Veeco, Santa Barbara, USA) was operated in tapping mode to probe the surface roughness and morphological changes of disc samples before and after difference lengths of OP treatment time. ..

    Article Title: Comparative Analysis of Silk Fibroin Membranes across Cross-Linking Methods: Processing and Characterization
    Article Snippet: All data processing was carried out with OriginLab software. .. Surface topography tests were performed on an atomic force microscope (AFM), Multimode SPM, (Veeco Instruments, USA) with an EV-scanner under ambient condition. ..

    Article Title: Surface Defect-Induced Dispersion and Stabilization of Monolayer MoS 2 Nanosheets in Polar Solvents
    Article Snippet: .. Atomic force microscope (AFM) images were acquired on a Multimode SPM (VEECO, USA) and a nanoIR2 fs (Anasys Instruments, USA) in tapping mode using a Si-tip cantilever, and the test sample was deposited on a mica wafer. .. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) mapping measurements were carried out using an SU8010 microscope (Hitachi, Japan).

    Article Title: White Painting Pigment as a Low-Cost Light Scattering Material for Bilayer Photoelectrodes of Dye-Sensitized Solar Cells
    Article Snippet: .. Similarly, the surface morphology of main-layer and light scattering layer was explored with an atomic force microscope (Multimode SPM, Veeco Metrology Group) at physics department in Brooklyn College, the Graduate Centre, the City University of New York, USA. ..

    other:

    Article Title: Study on the adhesive mechanism between the Ga doped ZnO thin film and the polycarbonate substrate
    Article Snippet: The Ga doped ZnO (GZO) film was deposited on the polymer substrate at room temperature by magnetron sputtering.. The resistivity is 8.9×10 Ω cm.. The average transmittance in the visible region is over 85%.

    Article Title: Effect of argon plasma-treated polyethylene terepthalate on ZnO:Al properties for flexible thin film silicon solar cells applications
    Article Snippet: The 0.4 μm-thick Aluminum-doped Zinc Oxide (ZnO:Al) films were deposited at 100 1C on polyethylene terephthalate (PET) substrates by Radio Frequency (RF) magnetron sputtering.. Prior to the AZO deposition, an Argon plasma treatment on the substrate surface was carried out by applying a RF bias power on it without intentional heating.. The parameters varied in the etching process were the plasma etching time from 0 to 360 s, the RF bias power from 50 to 250 W, and the gas flux from 3 to 5 sccm.

    Article Title: Tailoring of microstructure and optoelectronic properties of Aluminum doped Zinc Oxide changing gun tilt
    Article Snippet: The influence of gun tilt on microstructure and optoelectronic properties of ZnO:Al thin films deposited by magnetron sputtering onto transparent glass substrates was studied.. The structural and morphological film properties were investigated according to the cathode angle that placed in confocal geometry configuration inside sputtering system.. X-ray diffraction (XRD) and Atomic Force Microscope (AFM) were used, respectively.

    Article Title: Micro-level unloading behaviors of clay aggregates: A coarse-grained molecular dynamics study
    Article Snippet: • The effects of environments and loading conditions on the rebound were



    Similar Products

    atomic force microscope multimode spm  (Bruker Corporation)
    90
    Bruker Corporation atomic force microscope multimode spm
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscope Multimode Spm, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+dimension+icon/pmc12274505-322-10-15
    Average 90 stars, based on 1 article reviews
    atomic force microscope multimode spm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    atomic force microscope multimode spm  (Veeco)
    90
    Veeco atomic force microscope multimode spm
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscope Multimode Spm, supplied by Veeco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+afm/pmc12207278-65-9-11
    Average 90 stars, based on 1 article reviews
    atomic force microscope multimode spm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    atomic force microscope with a scanning spm probe of the nanoscope multimode type  (Veeco)
    90
    Veeco atomic force microscope with a scanning spm probe of the nanoscope multimode type
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscope With A Scanning Spm Probe Of The Nanoscope Multimode Type, supplied by Veeco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/microscope+with+a+scanning+spm+probe+of+the+nanoscope+multimode+type/pm40219271-125-25-28
    Average 90 stars, based on 1 article reviews
    atomic force microscope with a scanning spm probe of the nanoscope multimode type - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    atomic force microscope afm bruker multimode viii spm  (Bruker Corporation)
    90
    Bruker Corporation atomic force microscope afm bruker multimode viii spm
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscope Afm Bruker Multimode Viii Spm, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+dimension+icon/pm39697182-106-10-14
    Average 90 stars, based on 1 article reviews
    atomic force microscope afm bruker multimode viii spm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    atomic force microscopy multimode v8 spm nanoscope microscope  (Bruker Corporation)
    90
    Bruker Corporation atomic force microscopy multimode v8 spm nanoscope microscope
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscopy Multimode V8 Spm Nanoscope Microscope, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+dimension+icon/pm37896823-75-0-17
    Average 90 stars, based on 1 article reviews
    atomic force microscopy multimode v8 spm nanoscope microscope - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    atomic force microscope (afm) spm bruker multimode (mm8)  (Bruker Corporation)
    90
    Bruker Corporation atomic force microscope (afm) spm bruker multimode (mm8)
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Atomic Force Microscope (Afm) Spm Bruker Multimode (Mm8), supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+dimension+icon/pm37630240-387-11-12
    Average 90 stars, based on 1 article reviews
    atomic force microscope (afm) spm bruker multimode (mm8) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    nanoscope iv multimode atomic force scanning probe microscope using a model veeco-nanoscope iv multiple afm/spm  (Veeco)
    90
    Veeco nanoscope iv multimode atomic force scanning probe microscope using a model veeco-nanoscope iv multiple afm/spm
    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon <t>microscope</t> imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.
    Nanoscope Iv Multimode Atomic Force Scanning Probe Microscope Using A Model Veeco Nanoscope Iv Multiple Afm/Spm, supplied by Veeco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atomic+force+microscope+multimode+spm/atomic+force+microscope+afm/pmc09730306-49-24-25
    Average 90 stars, based on 1 article reviews
    nanoscope iv multimode atomic force scanning probe microscope using a model veeco-nanoscope iv multiple afm/spm - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon microscope imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.

    Journal: Nature Communications

    Article Title: The potassium channel K 2P 2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling

    doi: 10.1038/s41467-025-61816-9

    Figure Lengend Snippet: a Scheme of the experimental setup. Adoptive EAE was induced by injecting GFP + Th17 cells (green syringe) intravenously into Rag2 −/− cgn −/− mice. At disease score of 2, mice were anesthetized, equipped with a carotid artery catheter and prepared for intravital two-photon microscope imaging of the brainstem. Blood brain vessels were visualized by rhodamine-labeled dextran (red syringe). K 2P 2.1 inhibition was performed using spadin. b 3D-reconstructions with blood-vessels (red), CD4 + T cells (green) were calculated using two-photon imaging derived XYZ-stacks. Representative endoluminal crawling sequence (10 min) depicted from the two perspectives of endoluminal (upper panel) and perivascular (lower panel), respectively. c Representative sequence showing a CD4 + T cell (green) extravasating the blood vessel (lumen, red) shown from an extravascular sight. d The same blood vessel (red) was recorded for 30 min before (left), after spadin treatment (center) and after spadin treatment and blocking ICAM1 (right) with tracks from CD4 + T cells extravasating the vessel (green). e Mean number of CD4 + T cells extravasating the blood vessel in baseline (basel., dots) recordings ( N = 5) and after spadin and either isotype antibody (cubes, N = 7) or anti-ICAM1 injection ( N = 6). f EAE disease course of endothelial cell-specific Kcnk2 −/− ( Kcnk2 fl/fl xTie2 cre+ , blue ) and control ( Kcnk2 fl/fl xTie2 cre− , white ) mice with (gray cubes) or without (black dots) spadin treatment ( N = 8). g Analysis of EAE disease courses; Area under the curve (AUC) is shown for the different groups ( N = 8). Data are presented as mean ± SEM. N representing the number of individual mice. Statistical analysis using ( e ) 1-way ANOVA + Bonferroni correction and ( f , g ) 2-way ANOVA + Bonferroni correction with * p < 0.05 and *** p < 0.001. Exact p -values are listed in the Source Data file.

    Article Snippet: Mechanical stiffness of the endothelial cortex was determined using an atomic force microscope (MultiMode SPM, Bruker, Germany) as described elsewhere , .

    Techniques: Microscopy, Imaging, Labeling, Inhibition, Derivative Assay, Sequencing, Blocking Assay, Injection, Control