antibodies against asic3 (Alomone Labs)
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antibodies against asic3
Antibodies Against Asic3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asc/Anti-ASIC3+Antibody/pmc13616247-251-24-28
Average 93 stars, based on 33 article reviews
Antibodies Against Asic3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asc/Anti-ASIC3+Antibody/pmc13616247-251-24-28
Average 93 stars, based on 33 article reviews
antibodies against asic3 - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Cannabinoid CB1 Receptor in Nociceptors Mediates Postoperative Analgesia via ASIC3 Inhibition"
Article Title: Cannabinoid CB1 Receptor in Nociceptors Mediates Postoperative Analgesia via ASIC3 Inhibition
Journal: Advanced Science
doi: 10.1002/advs.77925
Figure Legend Snippet: ASIC3‐expressing nociceptors are activated in the DRG and contribute to incision‐induced postoperative pain. (A–C) Representative c‐Fos immunostaining images and quantification in WT DRG sections on postoperative day 1. Scale bar = 20 µm, 20 × objective, zoom = 1.97 ×. Unpaired t tests; n = 4 mice/group, 3–4 sections/mouse. (D–F) Western blot analysis of ASIC3 expression in WT mouse DRG and spinal dorsal horn (SDH) on postoperative day 1. Unpaired t tests; n = 5 mice/group. (G,H) ASIC3 protein levels in the DRG of littermate WT mice and Trpv1 ; Cnr1 fl/fl mice on day 1 post‐incision. Unpaired t tests; n = 5 mice/group. (I) i.pl. injection of the selective ASIC3 blocker APETx2 on postoperative day 1 robustly reverses mechanical allodynia in WT mice. (J) PWT following i.pl. injection of the ASIC3 blocker APETx2 in naïve Trpv1 ; Cnr1 fl/fl mice. Two‐way ANOVA with Sidak's test for multiple comparisons; * p < 0.05, ** p < 0.01. n = 6–11 mice/group; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle.
Techniques Used: Expressing, Immunostaining, Western Blot, Analysis, Injection
Figure Legend Snippet: The activation of CB 1 Rs inhibits ASIC3‐mediated nociceptive behavior. (A) Schematic of the GMQ test for assessing ASIC3‐driven nociception. (B,C) i.pl. GMQ evokes robust licking behavior and mechanical allodynia in WT mice. (D) Pretreatment with WIN (i.pl.) 15 min dose‐dependent inhibits 60 nmol GMQ‐induced licking behaviors in WT mice (left: time course; right: total licking time). (E,F) Pretreatment with WIN (i.pl.) fails to suppress GMQ‐induced licking in Adv ; Cnr1 fl/fl mice and Trpv1 ; Cnr1 fl/fl mice. (G) Pretreatment with WIN (i.pl.) 15 min alleviated GMQ‐induced mechanical allodynia in WT but not in Trpv1 ; Cnr1 fl/fl mice. (B–G) Two‐way ANOVA with Sidak correction for multiple comparisons or One‐way ANOVA with Tukey's multiple comparisons test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Sample sizes are indicated in parentheses. (H) Strategy of constructing Trpv1 ;iDTR mice. (I,J) Immunofluorescence verification of TRPV1 + neuron ablation in DRG. Scale bar = 50 µm, 20 × objective, zoom = 1.28 ×. Unpaired t tests; n = 6 mice/group; 4–5 sections/mouse. (K) GMQ‐induced licking behaviors attenuated in Trpv1 ;iDTR mice. Unpaired t tests; n = 10 mice/group; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle; WIN, WIN 55,212‐2.
Techniques Used: Activation Assay, Immunofluorescence
Figure Legend Snippet: CB 1 R and ASIC3 are co‐expressed in CGRP + neurons. (A) t‐SNE visualizations Trpv1 , Calca (encoding CGRP), Cnr1 , and Asic3 expression in adult mouse DRG neurons, based on a published single‐cell RNA‐seq dataset ( https://kleintools.hms.harvard.edu/tools/springViewer_1_6_dev.html?datasets/Sharma2019/adult ). (B,C) Representative combined ASIC3/CGRP immunofluorescence and Cnr1 RNAscope in situ hybridization images in DRG sections and quantification. Scale bars = 20 µm, 20 × objective, zoom = 2.84 ×; n = 5 mice/group, 3–5 sections/mouse. (D) UMAP plot revealing the Asic3 gene is rarely expressed in cells of the mice spinal cord. (E) Immunofluorescence staining of the spinal dorsal horn from WT mice for ASIC3 together with IB4, CGRP, and NF200. Scale bars = 100 µm, 20 × objective. Zoomed‐in views of the boxed regions, scale bars = 20 µm, zoom = 4.25 ×.
Techniques Used: Expressing, Single Cell, RNA Sequencing, Immunofluorescence, RNAscope, In Situ Hybridization, Staining
Figure Legend Snippet: CB 1 R physically interacts with ASIC3 and inhibits its channel function. (A‐C) Co‐immunoprecipitation (Co‐IP) of endogenous CB 1 R and ASIC3 proteins from WT mouse DRG lysates. Unpaired t tests; n = 5 samples/group; 3 mice/sample. (D‐F) Reciprocal Co‐IP of HA‐tagged CB 1 R and Flag‐tagged ASIC3 heterologously expressed in CHO cells. (G‐J) Representative traces and quantifications of pretreatment with 10 µM WIN (2 min) to 3 mM GMQ (10 s)‐induced inward current in CHO cells. Paired/unpaired t tests; n = 13–14 cells/groups. (K‐N) Representative traces and quantifications of pretreatment with 10 µM WIN (2 min) to the extracellular solution at pH 6.0‐induced inward current in CHO cells. Paired/unpaired t tests; n = 8–13 cells/groups; means ± SEM. Veh, vehicle; WIN, WIN 55,212‐2.
Techniques Used: Co-Immunoprecipitation Assay
Figure Legend Snippet: CB 1 R activation suppresses ASIC3‐mediated sensory neuron excitability. (A) Strategy of constructing Adv;Rosa26 GCaMP6f mice for calcium imaging. (B) Representative traces of GMQ and KCl‐evoked calcium transients. Scale bars = 50 µm, 20 × objective, zoom = 1.3 ×. (C) ΔF/F0 traces from cultured DRG neurons of Adv;Rosa26 GCaMP6f mice in response to sequential application of the ASIC3 agonist GMQ and KCl. (D) Quantification of the area under the curve (AUC) for GMQ‐evoked calcium transients. (E) Proportion of GMQ‐responsive neurons among total KCl‐responsive neurons. Unpaired t tests; n = 10–12 slides/group. (F,G) Representative action potential traces and quantification of firing frequency in cultured WT DRG neurons following treatment with GMQ (3 mM) alone or in combination with WIN (10 µM). Two‐way ANOVA with Sidak correction for multiple comparisons; n = 10–14 cells/group from 3 mice. (H,I) Representative traces and quantifications of rheobase of cultured WT DRG neurons. (J) Quantifications of resting membrane potentials (RMP) of cultured WT DRG neurons after different treatments. One‐way ANOVA with Tukey's multiple comparisons test; n = 10–14 cells/group from 3 mice; means ± SEM. Veh, vehicle; WIN, WIN 55,212‐2.
Techniques Used: Activation Assay, Imaging, Cell Culture, Membrane
Figure Legend Snippet: CB 1 R inhibits incision and ASIC3‐mediated ERK 1/2 phosphorylation. (A,B) Representative pERK 1/2 immunostaining images and quantifications in DRG sections of WT mice on postoperative day 1 and treated with intraplantar injection of WIN. Scale bar = 50 µm, 20 × objective, zoom = 1.28 ×. n = 5 mice/group, 3–4 sections/mouse. (C,D) Western blot analysis and quantification of pERK 1/2 in DRG from mice treated with intraplantar injection of APETx2 on postoperative day 1. n = 8 mice/group. (E,F) pERK 1/2 levels in DRGs from WT and ASIC3 KO mice on postoperative day 1. n = 8 mice/group. (G,H) Representative pERK 1/2 immunostaining and quantification in DRG after intraplantar injection of GMQ 30 min. Scale bar = 50 µm, 20 × objective, zoom = 1.46 ×. n = 4 mice/group, 4–5 sections/mouse. (I,J) Western blot analysis of pERK 1/2 in DRG from mice treated with GMQ alone or in combination with WIN. n = 4 mice/group. (K,L) pERK 1/2 levels in cultured DRG neurons stimulated with 3 mM GMQ in the presence or absence of 10 µM WIN. n = 9 wells/group. One‐way ANOVA with Tukey's multiple comparisons test; means ± SEM. Veh, vehicle; WIN, WIN 55,212‐2.
Techniques Used: Phospho-proteomics, Immunostaining, Injection, Western Blot, Analysis, Cell Culture
Figure Legend Snippet: Repeated targeting of the CB 1 R‐ASIC3 axis accelerates the resolution of incision‐induced postoperative pain. (A) Experimental timeline depicting plantar incision, daily intraplantar drug administration, and behavioral assessment. (B,C) Mechanical paw withdrawal threshold (PWT) and response frequency to a 0.4 g von Frey filament in mice receiving daily intraplantar injection of the CB 1 R agonist WIN or the CB 1 R antagonist AM251. (D,E) PWT and 0.4 g‐evoked response frequency in mice treated daily with the ASIC3 blocker APETx2 or the ASIC3 agonist GMQ. (F,G) PWT and 0.4 g‐evoked response frequency in WT mice and ASIC3 KO mice following plantar incision. Two‐way ANOVA with Sidak multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. Sample sizes are indicated in parentheses; means ± SEM. M, male; F, female; BL, baseline; Veh, vehicle; WIN, WIN 55,212‐2. (H) Proposed model of CB 1 R‐mediated postoperative analgesia in nociceptors through ASIC3 inhibition. In the mouse DRG, CB 1 R and ASIC3 are prominently co‐expressed in CGRP‐positive nociceptors. Plantar incision triggers an upregulation of ASIC3 and subsequent activation of ASIC3‐expressing neurons, thereby driving postoperative mechanical allodynia. Activation of nociceptor CB 1 R suppresses both ASIC3 channel function and ASIC3‐driven ERK 1/2 phosphorylation, thereby braking nociceptive signaling and accelerating pain resolution. This intrinsic CB 1 R‐ASIC3 regulatory module thus represents a pivotal endogenous analgesic mechanism and a promising therapeutic target for the management of postoperative pain. H, Created with BioRender.com, https://BioRender.com/eft35rm .
Techniques Used: Injection, Inhibition, Activation Assay, Expressing, Phospho-proteomics
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