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fluorescent microarray scanner arrayworx e-biochip reader  (Applied Precision Inc)

 
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    Structured Review

    Applied Precision Inc fluorescent microarray scanner arrayworx e-biochip reader
    a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA <t>microarray</t> scanner.
    Fluorescent Microarray Scanner Arrayworx E Biochip Reader, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arrayworx+e+scanner/fluorescent+microarray+scanner+arrayworx+e+biochip+reader/pmc04334502-73-7-13
    Average 90 stars, based on 1 article reviews
    fluorescent microarray scanner arrayworx e-biochip reader - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation"

    Article Title: A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0117744

    a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.
    Figure Legend Snippet: a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Techniques Used: Control, Labeling, Microarray

    Related Articles

    Incubation:

    Article Title: Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray
    Article Snippet: After another washing step, the wells were incubated for 30 min with 10% equine serum albumin (Sigma-Aldrich) in 2× SSC for 30 min. .. The slides were washed again and incubated with biotinylated Tyramide (1:50 in amplification buffer; TSA biotin system) for 10 min. After another washing step, the wells were incubated with streptavidin (2 μg ml −1 ) conjugated to Alexa Fluor 546 (Molecular Probes, Eugene, Oreg.) in 1× SSC-5× Denhardt's solution for 60 min. After a final wash in TNT buffer, the slides were spun dry and then imaged with an arrayWoRx e scanner (Applied Precision, Issaquah, Wash.). .. Image analysis software (softWoRx Tracker; Applied Precision) was used to quantify hybridization signals.

    Amplification:

    Article Title: Simultaneous Detection of Marine Fish Pathogens by Using Multiplex PCR and a DNA Microarray
    Article Snippet: After another washing step, the wells were incubated for 30 min with 10% equine serum albumin (Sigma-Aldrich) in 2× SSC for 30 min. .. The slides were washed again and incubated with biotinylated Tyramide (1:50 in amplification buffer; TSA biotin system) for 10 min. After another washing step, the wells were incubated with streptavidin (2 μg ml −1 ) conjugated to Alexa Fluor 546 (Molecular Probes, Eugene, Oreg.) in 1× SSC-5× Denhardt's solution for 60 min. After a final wash in TNT buffer, the slides were spun dry and then imaged with an arrayWoRx e scanner (Applied Precision, Issaquah, Wash.). .. Image analysis software (softWoRx Tracker; Applied Precision) was used to quantify hybridization signals.

    Software:

    Article Title: Transcriptional profiling of the LPS induced NF-κB response in macrophages
    Article Snippet: .. Microarrays were scanned using an ArrayWoRx e scanner and images were analysed with ArrayWoRx Software (Applied Precision LLC). ..



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    Image Search Results


    a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Journal: PLoS ONE

    Article Title: A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

    doi: 10.1371/journal.pone.0117744

    Figure Lengend Snippet: a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

    Article Snippet: The microfluidic device was scanned using a fluorescent microarray scanner (ArrayWorx e-Biochip Reader, Applied Precision, USA).

    Techniques: Control, Labeling, Microarray