arrayworx®e biochip fluorescence reader (Applied Precision Inc)
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Arrayworx®E Biochip Fluorescence Reader, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Centrifugation:Article Title: Identification of a Novel One-carbon Metabolism Regulon in Saccharomyces cerevisiae Article Snippet: Hybridization was performed at 37 °C for 14–16 h in DIG EasyHyb (Roche Diagnostics) with 0.5 mg/ml E. coli tRNA and 0.5 mg/ml herring sperm DNA. .. The slides were washed 3 times in 1 SSC (0.15 M sodium chloride, 0.015 M trisodium citrate), 0.1% SDS at 50 °C, followed by rinsing in 1 SSC, dried by centrifugation, and scanned in an Applied Precision Microarray:Article Title: Comparative modeling and analysis of microfluidic and conventional DNA microarrays. Article Snippet: A theoretical analysis was developed to predict molecular hybridization rates for microarrays where samples flow through microfluidic channels and for conventional microarrays where samples remain stationary during hybridization.. The theory was validated by using a multiplexed microfluidic microarray where eight samples were hybridized simultaneously against eight probes using 60-mer DNA strands.. Mass transfer coefficients ranged over three orders of magnitude where either kinetic reaction rates or molecular diffusion rates controlled overall hybridization rates. Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays Article Snippet: .. In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays Article Snippet: .. Microarray Imaging and Quantification In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an Staining:Article Title: Cellular retinoic acid binding protein I: expression and functional influence in renal cell carcinoma. Article Snippet: Despite the known anti-proliferative and tumor-suppressive effects seen with retinoic acid (RA), treatment of metastatic renal cell carcinoma (RCC) failed to meet the initial expectations.. As the exact mechanisms of action of RA and especially the role of the cellular RA binding proteins (CRABP) have not been elucidated yet, we investigated the expression of CRABP-I and its potential infl uence on RA response in RCC.. Real-time RT-PCR analysis disclosed a signifi cant lack of CRABP-I expression in four RCC cell lines and 12 primary RCC samples; in contrast, high expression levels were found in the respective adjacent normal kidney tissue. Imaging:Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays Article Snippet: .. Microarray Imaging and Quantification In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an |
