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Applied Precision Inc arrayworx®e biochip fluorescence reader
Arrayworx®E Biochip Fluorescence Reader, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/arrayworx+e+biochip+reader/arrayworxe+biochip+fluorescence+reader/us11885802-160-5-9
Average 90 stars, based on 1 article reviews
arrayworx®e biochip fluorescence reader - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Centrifugation:

Article Title: Identification of a Novel One-carbon Metabolism Regulon in Saccharomyces cerevisiae
Article Snippet: Hybridization was performed at 37 °C for 14–16 h in DIG EasyHyb (Roche Diagnostics) with 0.5 mg/ml E. coli tRNA and 0.5 mg/ml herring sperm DNA. .. The slides were washed 3 times in 1 SSC (0.15 M sodium chloride, 0.015 M trisodium citrate), 0.1% SDS at 50 °C, followed by rinsing in 1 SSC, dried by centrifugation, and scanned in an Applied Precision ArrayWoRx E Biochip Reader. ..

Microarray:

Article Title: Comparative modeling and analysis of microfluidic and conventional DNA microarrays.
Article Snippet: A theoretical analysis was developed to predict molecular hybridization rates for microarrays where samples flow through microfluidic channels and for conventional microarrays where samples remain stationary during hybridization.. The theory was validated by using a multiplexed microfluidic microarray where eight samples were hybridized simultaneously against eight probes using 60-mer DNA strands.. Mass transfer coefficients ranged over three orders of magnitude where either kinetic reaction rates or molecular diffusion rates controlled overall hybridization rates.

Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays
Article Snippet: .. In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an ArrayWoRx e BioChip reader (Applied Precision, LLC, Issaquah, WA). ..

Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays
Article Snippet: .. Microarray Imaging and Quantification In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an ArrayWoRx e BioChip reader (Applied Precision, LLC, Issaquah, WA). ..

Staining:

Article Title: Cellular retinoic acid binding protein I: expression and functional influence in renal cell carcinoma.
Article Snippet: Despite the known anti-proliferative and tumor-suppressive effects seen with retinoic acid (RA), treatment of metastatic renal cell carcinoma (RCC) failed to meet the initial expectations.. As the exact mechanisms of action of RA and especially the role of the cellular RA binding proteins (CRABP) have not been elucidated yet, we investigated the expression of CRABP-I and its potential infl uence on RA response in RCC.. Real-time RT-PCR analysis disclosed a signifi cant lack of CRABP-I expression in four RCC cell lines and 12 primary RCC samples; in contrast, high expression levels were found in the respective adjacent normal kidney tissue.

Imaging:

Article Title: Photocleavage Based Affinity Purification and Printing of Cell-free Expressed Proteins: Application to Proteome Microarrays
Article Snippet: .. Microarray Imaging and Quantification In all cases, following the final washing, microarray substrates were dried by placing them in 50 mL conical tubes padded at the bottom with lint-free paper towels and spun in a standard swing-bucket clinical centrifuge at 2,500 rpm for 1 min. Substrates were imaged on an ArrayWoRx e BioChip reader (Applied Precision, LLC, Issaquah, WA). ..



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Image Search Results


a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

Journal: PLoS ONE

Article Title: A Microfluidic Platform for High-Throughput Multiplexed Protein Quantitation

doi: 10.1371/journal.pone.0117744

Figure Lengend Snippet: a) microfluidic design: the device consists of two PDMS layers: flow (blue) and control (red). The chip is an array of eight rows by 48 columns for 384 unit cells. Each unit cell is composed of: two antibody chambers divided by a reaction chamber (1–2), 4 MITOMI buttons (3), a valve that segregates the unit cells (4), a valve that separates antibody and reaction chambers (5) and a valve for releasing pressure in the antibody chambers (6). b) The PDMS chip is aligned to an epoxy-functionalized slide onto which primary and secondary antibodies were spotted. c) Assay details: schematic of the unit cell and cross section of a button region: i) functionalization of the surface: BSA-biotin is flowed though the chip followed by neutravidin. Next, the buttons are closed and BSA-biotin flowed again to passivate all neutravidin molecules except for those located underneath the MITOMI buttons, ii) the biotinylated primary antibody is allowed to diffuse into the MITOMI detection chamber and is bound by neutravidin immobilizing it in the MITOMI detection regions, iii) the sample is flown through the device and antigens are captured by the surface immobilized antibodies, iv) finally, the fluorescently labeled secondary antibody is allowed to diffuse into the MITOMI area, binds to the antigen if present, and is trapped by MITOMI. The entire device is then quantitated using a DNA microarray scanner.

Article Snippet: The microfluidic device was scanned using a fluorescent microarray scanner (ArrayWorx e-Biochip Reader, Applied Precision, USA).

Techniques: Control, Labeling, Microarray