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apoa i  (Proteintech)


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    Structured Review

    Proteintech apoa i
    Apoa I, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoa+i/Apolipoprotein+AI+Antibody/pmc12787056-220-42-44
    Average 94 stars, based on 52 article reviews
    apoa i - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXR α -mediated ABCA1 expression
    Article Snippet: Antibodies against ALDH2 (15310-1-AP), LDLR (10785-1-AP), LXRα (14351-1-AP), GAPDH (60004-1-Ig), DYKDDDDK Tag (80010-1-RR), ABCG5 (27722-1-AP), ApoA-I (66206-1-Ig), His-Tag (66005-1-1g), lamin B1 (12987-1-AP), and α-tubulin (11224-1-AP) were purchased from Proteintech.

    Clinical Proteomics:

    Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate
    Article Snippet: .. Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    Article Title: Effect of Phospholipid Transfer Protein on Plasma Sphingosine-1-Phosphate.
    Article Snippet: .. Plasma (0.2 μl) was separated by 4-15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    SDS-Gel:

    Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate
    Article Snippet: .. Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    Article Title: Effect of Phospholipid Transfer Protein on Plasma Sphingosine-1-Phosphate.
    Article Snippet: .. Plasma (0.2 μl) was separated by 4-15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    Electrophoresis:

    Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate
    Article Snippet: .. Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    Article Title: Effect of Phospholipid Transfer Protein on Plasma Sphingosine-1-Phosphate.
    Article Snippet: .. Plasma (0.2 μl) was separated by 4-15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP). ..

    Western Blot:

    Article Title: Lipoproteins and Exosomes as Novel Carriers of Soluble Fms-Like Tyrosine Kinase-1 and Placental Growth Factor During Pregnancy
    Article Snippet: .. B, Western blot of apolipoprotein A-I (ApoA-I), apolipoprotein B (ApoB), and the exosomal marker CD9 in the aforementioned FPLC fractions, making use of primary antibodies directed against ApoA-I (Proteintech, 14427-1-AP, 1:1000 dilution), ApoB (Proteintech, 20578-1-AP, 1:1000 dilution), and CD9 (Invitrogen, 13-0098-82, 1:1000 dilution). .. C, Pull-down of anti-PlGF (2 μg/mL; R&D Systems) or anti-Flt-1 (2 μg/mL; R&D Systems), added to a serum pool for 30 minutes at room temperature, by Dynabeads Streptavidin (Thermo Fisher), followed by (Continued ) D ow nloaded from http://ahajournals.org by on N ovem ber 19, 2024 RE SE AR CH LE TT ER e134 October 2024 Hypertension.

    Article Title: Gut Microbiota-Derived Propionic Acid Mediates ApoA-I-Induced Amelioration of MASLD via Activation of GPR43–Ca 2+ –CAMKII–ATGL Hepatic Lipolysis
    Article Snippet: The protein concentration was quantified via a BCA assay (#23227; Thermo Fisher Scientific, Waltham, MA, USA). .. Protein levels were evaluated by Western blotting using antibodies against phospho-CaMKII (#12716T; Cell Signaling Technology (CST), Danvers, MA, USA), CaMKII (#4436T; CST, Danvers, MA, USA), phospho-ATGL (#ab317611; Abcam, Cambridge, UK), ATGL (#55190-1-AP; Proteintech, Rosemont, IL, USA), β-actin (#60008-1-Ig; Proteintech, Rosemont, IL, USA), apoA-I (#14427-1-AP; Proteintech, Rosemont, IL, USA), GPR43 (#84544-1-RR; Proteintech, Rosemont, IL, USA) and vinculin (#26520-1-AP; Proteintech, Rosemont, IL, USA). .. The band intensity was analysed using ImageJ software (version 1.53; National Institutes of Health, Bethesda, MD, USA).

    Marker:

    Article Title: Lipoproteins and Exosomes as Novel Carriers of Soluble Fms-Like Tyrosine Kinase-1 and Placental Growth Factor During Pregnancy
    Article Snippet: .. B, Western blot of apolipoprotein A-I (ApoA-I), apolipoprotein B (ApoB), and the exosomal marker CD9 in the aforementioned FPLC fractions, making use of primary antibodies directed against ApoA-I (Proteintech, 14427-1-AP, 1:1000 dilution), ApoB (Proteintech, 20578-1-AP, 1:1000 dilution), and CD9 (Invitrogen, 13-0098-82, 1:1000 dilution). .. C, Pull-down of anti-PlGF (2 μg/mL; R&D Systems) or anti-Flt-1 (2 μg/mL; R&D Systems), added to a serum pool for 30 minutes at room temperature, by Dynabeads Streptavidin (Thermo Fisher), followed by (Continued ) D ow nloaded from http://ahajournals.org by on N ovem ber 19, 2024 RE SE AR CH LE TT ER e134 October 2024 Hypertension.

    Fast Protein Liquid Chromatography:

    Article Title: Lipoproteins and Exosomes as Novel Carriers of Soluble Fms-Like Tyrosine Kinase-1 and Placental Growth Factor During Pregnancy
    Article Snippet: .. B, Western blot of apolipoprotein A-I (ApoA-I), apolipoprotein B (ApoB), and the exosomal marker CD9 in the aforementioned FPLC fractions, making use of primary antibodies directed against ApoA-I (Proteintech, 14427-1-AP, 1:1000 dilution), ApoB (Proteintech, 20578-1-AP, 1:1000 dilution), and CD9 (Invitrogen, 13-0098-82, 1:1000 dilution). .. C, Pull-down of anti-PlGF (2 μg/mL; R&D Systems) or anti-Flt-1 (2 μg/mL; R&D Systems), added to a serum pool for 30 minutes at room temperature, by Dynabeads Streptavidin (Thermo Fisher), followed by (Continued ) D ow nloaded from http://ahajournals.org by on N ovem ber 19, 2024 RE SE AR CH LE TT ER e134 October 2024 Hypertension.



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    Image Search Results


    Workflow for EV enrichment from 4 ml (A) and 0.5 ml (B). EV markers (CD63 and Syntenin-1) and plasma contaminant proteins (Albumin and ApoA1(shown in red)) by western blot analysis (panel C). Starting plasma volume, final volume post concentration with the centrifugal filter and gel loading volume were used to calculate the concentration factor and equivalent plasma starting amounts indicated above figure.

    Journal: bioRxiv

    Article Title: Combining Anion Exchange and Size Exclusion Chromatography for Extracellular Vesicle Enrichment from Small Volumes of Human and Mouse Plasma for Quantitative Proteomics

    doi: 10.64898/2026.03.11.711200

    Figure Lengend Snippet: Workflow for EV enrichment from 4 ml (A) and 0.5 ml (B). EV markers (CD63 and Syntenin-1) and plasma contaminant proteins (Albumin and ApoA1(shown in red)) by western blot analysis (panel C). Starting plasma volume, final volume post concentration with the centrifugal filter and gel loading volume were used to calculate the concentration factor and equivalent plasma starting amounts indicated above figure.

    Article Snippet: Membranes were blocked in 5% skim milk in PBS at room temperature for 1 hour before overnight incubation with primary antibodies Syntenin (Abcam, cat# ab133267), CD63 (Abcam, cat#ab134045), CD63 (Abcam, cat#ab8219), Albumin (Cell Signalling Technology, cat#4929S), Calnexin (Cell Signalling Technology, cat#2679S) ApoA1 (Santa Cruz, cat# SC-376818).

    Techniques: Clinical Proteomics, Western Blot, Concentration Assay

    SEC Void optimisation. Pooled SEC fractions 1-4 collected using a 2.7 mL void volume (1-4A) and a 2.8 mL void volume (1-4B) were prepared in triplicate from 0.5 mL plasma. Western blot analysis of contaminant proteins Albumin (green) and lipoprotein ApoA1 (red) (A) with 0.5 µl plasma included as a positive control (+). EV markers CD63 and syntenin-1 and small EV exclusion marker Calnexin (B). Samples (0.5 ml and 0.4 ml) from Experiment 3.2 included as a western blot control and 2µl platelet lysate included as a calnexin positive control (+). Electron microscopy (C) visualisation and size and concentration histogram (D) of EV representative SEC sample (Void 2.7 ml).

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    doi: 10.64898/2026.03.11.711200

    Figure Lengend Snippet: SEC Void optimisation. Pooled SEC fractions 1-4 collected using a 2.7 mL void volume (1-4A) and a 2.8 mL void volume (1-4B) were prepared in triplicate from 0.5 mL plasma. Western blot analysis of contaminant proteins Albumin (green) and lipoprotein ApoA1 (red) (A) with 0.5 µl plasma included as a positive control (+). EV markers CD63 and syntenin-1 and small EV exclusion marker Calnexin (B). Samples (0.5 ml and 0.4 ml) from Experiment 3.2 included as a western blot control and 2µl platelet lysate included as a calnexin positive control (+). Electron microscopy (C) visualisation and size and concentration histogram (D) of EV representative SEC sample (Void 2.7 ml).

    Article Snippet: Membranes were blocked in 5% skim milk in PBS at room temperature for 1 hour before overnight incubation with primary antibodies Syntenin (Abcam, cat# ab133267), CD63 (Abcam, cat#ab134045), CD63 (Abcam, cat#ab8219), Albumin (Cell Signalling Technology, cat#4929S), Calnexin (Cell Signalling Technology, cat#2679S) ApoA1 (Santa Cruz, cat# SC-376818).

    Techniques: Clinical Proteomics, Western Blot, Positive Control, Marker, Control, Electron Microscopy, Concentration Assay