1x vectcell tm trolox (Vector Laboratories)
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Vector Laboratories
1x vectcell tm trolox
1x Vectcell Tm Trolox, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antifade+reagent/VectaCell+Trolox+Antifade+Reagent+for+Live+Cell+Imaging/bio_rxiv__64898__2026__04__21__719874-463-21-26
Average 93 stars, based on 17 article reviews
1x Vectcell Tm Trolox, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antifade+reagent/VectaCell+Trolox+Antifade+Reagent+for+Live+Cell+Imaging/bio_rxiv__64898__2026__04__21__719874-463-21-26
Average 93 stars, based on 17 article reviews
1x vectcell tm trolox - by Bioz Stars,
2026-10
93/100 stars
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Immunofluorescence:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Staining:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: CD44 antagonism after traumatic brain injury influences the adult neurogenic niche and behavioral outcomes Article Snippet: For GFAP, slices were stained with mouse anti-GFAP-Cy3 conjugated antibody (Sigma-Aldrich, St. Louis, MO; #C9205; RRID: AB_476889; 1:500) overnight at room temperature. .. For DCX, slices first underwent antigen retrieval in citrate buffer at 45° C for one hour and were subsequently stained with primary goat anti-DCX antibody (Santa Cruz Biotechnology, Dallas, TX; #sc-8066; RRID: AB_2088494; 1:200) overnight at room temperature, followed by secondary biotinylated donkey anti-goat IgG-Alexafluor-555 (Invitrogen, Waltham, MA; #A21432; RRID: AB_2535853; 1:200) for 1.5 h. For co-staining, GFAP was stained using rabbit anti-GFAP primary antibody (Sigma-Aldrich, St. Louis, MO; #G9269; RRID: AB_477035; 1:500) overnight at room temperature, followed by goat anti-rabbit IgG-AlexaFluor-488 (Invitrogen, Waltham, MA; #A21432; RRID: AB_2534069; 1:200) for 1.5 h. All slices were mounted and cover-slipped with Isolation:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Incubation:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets. Article Snippet: .. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked inMABT buffer (11.6 g/LMaleic acid, 8.7 g/L NaCl, 0.1%Tween20)with 10%FBS for 2 h. Tissueswere then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: Long noncoding RNA-dependent control of Myc transcriptional bursting Article Snippet: .. The next day, cells were washed 2 times for 30 min at 30°C in Wash Buffer A, incubated in Wash Buffer B (Stellaris, LGC Biosciences) for 5 min at RT, mounted in Blocking Assay:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets. Article Snippet: .. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked inMABT buffer (11.6 g/LMaleic acid, 8.7 g/L NaCl, 0.1%Tween20)with 10%FBS for 2 h. Tissueswere then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Imaging:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets. Article Snippet: .. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked inMABT buffer (11.6 g/LMaleic acid, 8.7 g/L NaCl, 0.1%Tween20)with 10%FBS for 2 h. Tissueswere then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Confocal Microscopy:Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets Article Snippet: After rinsing with PBS to stop the chromogenic reaction, slides were counterstained with hematoxylin QS (Vector Laboratories) for 2 min before analysis by microscopy (Lecia Aperio VERSA 8). .. For whole-mount immunofluorescence staining, freshly isolated islets, scWAT or epiWAT tissue lumps were fixed in 4% PFA at 4 °C for 1 h, and permeabilized with PBST buffer (0.1% Triton X-100 in PBS) for 30 min. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked in MABT buffer (11.6 g/L Maleic acid, 8.7 g/L NaCl, 0.1% Tween 20) with 10% FBS for 2 h. Tissues were then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with Article Title: Endothelial IRE1α promotes thrombospondin-1 mRNA decay and supports metabolic stress adaptation of pancreatic islets. Article Snippet: .. Islets were blocked in PBST buffer with 10% FBS for 2 h, and WAT tissues were blocked inMABT buffer (11.6 g/LMaleic acid, 8.7 g/L NaCl, 0.1%Tween20)with 10%FBS for 2 h. Tissueswere then incubated with anti-Insulin (Abcam, ab10983), anti-CD31 (BD Pharmingen, 553372), anti-Perilipin-1 (CST, 3470), or anti-Ki67 (BD, 612472; CST, 9127) primary antibody in the blocking buffer at 4 °C for 24 h, followed by washing 3 times with PBST buffer before incubation with the secondary antibody (Thermo Fisher Scientific, A-11012) at a 1:200 dilution at 4 °C for 24 h. Tissues were mounted with |