vamp2 rb (Synaptic Systems)
Synaptic Systems is a verified supplier 
Synaptic Systems manufactures this product 
94
Structured Review
Synaptic Systems
vamp2 rb
![( A ) Confocal image of a VGLUT2 + dopamine neuron in primary midbrain culture from HA-VMAT2 KI mice. Scale bar, 10 μm. ( B ) Magnified regions from white boxes in (A) and fluorescence intensity profiles of HA-VMAT2, VGLUT2, and TH along a line ROI, showing overlapping VGLUT2 and HA-VMAT2 peaks. Scale bars, 3 μm. ( C ) Scatterplot of HA-VMAT2 and VGLUT2 signal in individual presynaptic varicosities from VGLUT2 + dopamine neurons (colors indicate individual neurons). ( D ) Quantification of HA-VMAT2 in the presynaptic terminals of dopamine-only versus VMAT2 + /VGLUT2 + neurons and VGLUT2 in glutamate-only versus VMAT2 + /VGLUT2 + neurons. DA, dopamine; Glu, glutamate. Each data point represents mean of ROIs per image ( n = 19/21 for HA-VMAT2 and 11/20 for VGLUT2 from two independent cultures). Data indicate mean ± SEM; *** P = 0.0006 by Mann-Whitney test. Confocal images of a dopamine/glutamate neuron ( E ) or dopamine-only neuron ( F ) stained for TH (not shown), synaptophysin, HA-VMAT2, and VGLUT2. Gray arrowheads in (E) point to VGLUT2 + /VMAT2 + varicosities and white arrowheads in (F) to VMAT2-only terminals. In both images, orange arrowheads point to VGLUT2-only terminals likely from glutamate neurons. Scale bars, 10 μm. Quantification of <t>VAMP2</t> ( G ), Slc10A4 ( H ), syt1 ( I ), syt2 ( J ), SCAMP5 ( K ), synaptophysin ( L ), and CSPα ( M ) in presynaptic terminals of dopamine-only or VMAT2 + /VGLUT2 + neurons. Each data point represents mean of individual ROIs per image [ n = 17/11 (G), 15/8 (H), 8/10 (I), 11/6 (J), 11/8 (K), 24/17 (L), or 22/14 (M)]. Quantifications were performed on images from two [(G), (H), (J), and (K)] or three [(I), (L), and (M)] independent cultures. Data indicate mean ± SEM. ** P < 0.01 by unpaired t test (K and M) or Mann-Whitney test (L). Scatterplots showing correlations between Slc10A4 ( N ) and synaptophysin ( O ) with HA-VMAT2 (magenta) or VGLUT2 (orange) in presynaptic terminals containing both transporters.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4401/pmc13394401/pmc13394401__sciadv.adz6836-f5.jpg)
Vamp2 Rb, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-vamp2/104+202/pmc13394401-349-61-64
Average 94 stars, based on 71 article reviews
Vamp2 Rb, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-vamp2/104+202/pmc13394401-349-61-64
Average 94 stars, based on 71 article reviews
vamp2 rb - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Synaptic vesicles that store monoamines and glutamate differ in protein composition"
Article Title: Synaptic vesicles that store monoamines and glutamate differ in protein composition
Journal: Science Advances
doi: 10.1126/sciadv.adz6836
Figure Legend Snippet: ( A ) Confocal image of a VGLUT2 + dopamine neuron in primary midbrain culture from HA-VMAT2 KI mice. Scale bar, 10 μm. ( B ) Magnified regions from white boxes in (A) and fluorescence intensity profiles of HA-VMAT2, VGLUT2, and TH along a line ROI, showing overlapping VGLUT2 and HA-VMAT2 peaks. Scale bars, 3 μm. ( C ) Scatterplot of HA-VMAT2 and VGLUT2 signal in individual presynaptic varicosities from VGLUT2 + dopamine neurons (colors indicate individual neurons). ( D ) Quantification of HA-VMAT2 in the presynaptic terminals of dopamine-only versus VMAT2 + /VGLUT2 + neurons and VGLUT2 in glutamate-only versus VMAT2 + /VGLUT2 + neurons. DA, dopamine; Glu, glutamate. Each data point represents mean of ROIs per image ( n = 19/21 for HA-VMAT2 and 11/20 for VGLUT2 from two independent cultures). Data indicate mean ± SEM; *** P = 0.0006 by Mann-Whitney test. Confocal images of a dopamine/glutamate neuron ( E ) or dopamine-only neuron ( F ) stained for TH (not shown), synaptophysin, HA-VMAT2, and VGLUT2. Gray arrowheads in (E) point to VGLUT2 + /VMAT2 + varicosities and white arrowheads in (F) to VMAT2-only terminals. In both images, orange arrowheads point to VGLUT2-only terminals likely from glutamate neurons. Scale bars, 10 μm. Quantification of VAMP2 ( G ), Slc10A4 ( H ), syt1 ( I ), syt2 ( J ), SCAMP5 ( K ), synaptophysin ( L ), and CSPα ( M ) in presynaptic terminals of dopamine-only or VMAT2 + /VGLUT2 + neurons. Each data point represents mean of individual ROIs per image [ n = 17/11 (G), 15/8 (H), 8/10 (I), 11/6 (J), 11/8 (K), 24/17 (L), or 22/14 (M)]. Quantifications were performed on images from two [(G), (H), (J), and (K)] or three [(I), (L), and (M)] independent cultures. Data indicate mean ± SEM. ** P < 0.01 by unpaired t test (K and M) or Mann-Whitney test (L). Scatterplots showing correlations between Slc10A4 ( N ) and synaptophysin ( O ) with HA-VMAT2 (magenta) or VGLUT2 (orange) in presynaptic terminals containing both transporters.
Techniques Used: Fluorescence, MANN-WHITNEY, Staining
Related Articles
Western Blot:Article Title: The proteomic landscape of synaptic diversity across brain regions and cell types. Article Snippet: The following primary antibodies and corresponding dilution factors were used: anti-PSMA3 (Enzo, BML-PW8110-0100 – ms, 1:1000), anti-PSMB8 (Enzo, BML-PW8845-0100 –ms, 1:1000), anti-PSMB5 (CST, 12919 – rb, 1:1000), anti-PSME1 (Abcam, ab155091 – rb, 1:1000), anti-Histone H3 (Abcam, ab1791 – rb, 1:1000), Article Title: Article Snippet: Primary antibodies: Article Title: Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Article Snippet: The antibodies used in Article Title: Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes Article Snippet: The following antibodies were KO validated: Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity Article Snippet: The primary antibody used were: anti-actin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease. Article Snippet: The antibodies used for the western blot were Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer’s disease Article Snippet: The antibodies used for the western blot were Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity. Article Snippet: The primary antibody used were: antiactin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Milk:Article Title: The proteomic landscape of synaptic diversity across brain regions and cell types. Article Snippet: The following primary antibodies and corresponding dilution factors were used: anti-PSMA3 (Enzo, BML-PW8110-0100 – ms, 1:1000), anti-PSMB8 (Enzo, BML-PW8845-0100 –ms, 1:1000), anti-PSMB5 (CST, 12919 – rb, 1:1000), anti-PSME1 (Abcam, ab155091 – rb, 1:1000), anti-Histone H3 (Abcam, ab1791 – rb, 1:1000), Article Title: Article Snippet: Primary antibodies: Article Title: Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Article Snippet: The antibodies used in Article Title: Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes Article Snippet: The following antibodies were KO validated: Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity Article Snippet: The primary antibody used were: anti-actin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease. Article Snippet: The antibodies used for the western blot were Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer’s disease Article Snippet: The antibodies used for the western blot were Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity. Article Snippet: The primary antibody used were: antiactin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Immunocytochemistry:Article Title: The proteomic landscape of synaptic diversity across brain regions and cell types. Article Snippet: The following primary antibodies and corresponding dilution factors were used: anti-PSMA3 (Enzo, BML-PW8110-0100 – ms, 1:1000), anti-PSMB8 (Enzo, BML-PW8845-0100 –ms, 1:1000), anti-PSMB5 (CST, 12919 – rb, 1:1000), anti-PSME1 (Abcam, ab155091 – rb, 1:1000), anti-Histone H3 (Abcam, ab1791 – rb, 1:1000), Article Title: Article Snippet: Primary antibodies: Article Title: Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Article Snippet: The antibodies used in Article Title: Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes Article Snippet: The following antibodies were KO validated: Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity Article Snippet: The primary antibody used were: anti-actin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease. Article Snippet: The antibodies used for the western blot were Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer’s disease Article Snippet: The antibodies used for the western blot were Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity. Article Snippet: The primary antibody used were: antiactin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Immunohistochemistry:Article Title: The proteomic landscape of synaptic diversity across brain regions and cell types. Article Snippet: The following primary antibodies and corresponding dilution factors were used: anti-PSMA3 (Enzo, BML-PW8110-0100 – ms, 1:1000), anti-PSMB8 (Enzo, BML-PW8845-0100 –ms, 1:1000), anti-PSMB5 (CST, 12919 – rb, 1:1000), anti-PSME1 (Abcam, ab155091 – rb, 1:1000), anti-Histone H3 (Abcam, ab1791 – rb, 1:1000), Article Title: Article Snippet: Primary antibodies: Article Title: Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Article Snippet: The antibodies used in Article Title: Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes Article Snippet: The following antibodies were KO validated: Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity Article Snippet: The primary antibody used were: anti-actin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease. Article Snippet: The antibodies used for the western blot were Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer’s disease Article Snippet: The antibodies used for the western blot were Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity. Article Snippet: The primary antibody used were: antiactin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Enzyme-linked Immunosorbent Assay:Article Title: The proteomic landscape of synaptic diversity across brain regions and cell types. Article Snippet: The following primary antibodies and corresponding dilution factors were used: anti-PSMA3 (Enzo, BML-PW8110-0100 – ms, 1:1000), anti-PSMB8 (Enzo, BML-PW8845-0100 –ms, 1:1000), anti-PSMB5 (CST, 12919 – rb, 1:1000), anti-PSME1 (Abcam, ab155091 – rb, 1:1000), anti-Histone H3 (Abcam, ab1791 – rb, 1:1000), Article Title: Article Snippet: Primary antibodies: Article Title: Cd99l2 regulates excitatory synapse development and restrains immediate-early gene activation. Article Snippet: The antibodies used in Article Title: Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes Article Snippet: The following antibodies were KO validated: Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity Article Snippet: The primary antibody used were: anti-actin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer's disease. Article Snippet: The antibodies used for the western blot were Article Title: Neuronal CD59 isoforms IRIS-1 and IRIS-2 as regulators of neurotransmitter release with implications for Alzheimer’s disease Article Snippet: The antibodies used for the western blot were Article Title: The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity. Article Snippet: The primary antibody used were: antiactin (1:1000; A2066, Sigma), anti-TH (1:1000; ab112, Abcam), |
