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anti mouse thy1 2 cd90  (Bio-Rad)


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    Bio-Rad anti mouse thy1 2 cd90
    Anti Mouse Thy1 2 Cd90, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+thy1+2/Mouse+anti+Mouse+CD90/pmc12721432-65-18-20
    Average 93 stars, based on 164 article reviews
    anti mouse thy1 2 cd90 - by Bioz Stars, 2026-11
    93/100 stars

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    Incubation:

    Article Title: Retinal Müller Cell-Released Exosomal MiR-92a-3p Delivers Interleukin-17A Signal by Targeting Notch-1 to Promote Diabetic Retinopathy.
    Article Snippet: .. RGCs were then selected by incubation on a dish coated with goat anti-mouse IgG and mouse anti-Thy1.2 (clone F7D5; AbD Serotec, Oxford, UK). .. After washing with Earle’s balanced salt solution, the RGCs were released from the final panning dish with trypsin (Sigma-Aldrich, St. Louis, MO, USA) and the trypsinization was terminated using Neurobasal medium (Gibco, Rockville, MD, USA) containing 10% FBS.

    Article Title: Retinal Müller Cell-Released Exosomal MiR-92a-3p Delivers Interleukin-17A Signal by Targeting Notch-1 to Promote Diabetic Retinopathy
    Article Snippet: .. RGCs were then selected by incubation on a dish coated with goat anti-mouse IgG and mouse anti-Thy1.2 (clone F7D5; AbD Serotec, Oxford, UK). .. After washing with Earle’s balanced salt solution, the RGCs were released from the final panning dish with trypsin (Sigma-Aldrich, St. Louis, MO, USA) and the trypsinization was terminated using Neurobasal medium (Gibco, Rockville, MD, USA) containing 10% FBS.

    Purification:

    Article Title: Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (BioRad, Hercules, CA; Cat #MCA02R) and mouse anti-GalC (Millipore, Burlington, MA; Cat #MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation media containing PDGF-AA and NT-3 (PreproTech, Rocky Hill, NJ ).

    Article Title: GAIN domain–mediated cleavage is required for activation of G protein–coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (Serotec, catalog no. MCA02R) and mouse anti-GalC (Millipore, catalog no. MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation medium containing (PDGF-AA) Platelet-derived growth factor composed of two A subunits and NT-3 (PeproTech).

    Article Title: Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (BioRad, Hercules, CA; Cat #MCA02R) and mouse anti-GalC (Millipore, Burlington, MA; Cat #MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation media containing PDGF-AA and NT-3 (PreproTech, Rocky Hill, NJ ).

    Selection:

    Article Title: Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (BioRad, Hercules, CA; Cat #MCA02R) and mouse anti-GalC (Millipore, Burlington, MA; Cat #MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation media containing PDGF-AA and NT-3 (PreproTech, Rocky Hill, NJ ).

    Article Title: GAIN domain–mediated cleavage is required for activation of G protein–coupled receptor 56 (GPR56) by its natural ligands and a small-molecule agonist
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (Serotec, catalog no. MCA02R) and mouse anti-GalC (Millipore, catalog no. MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation medium containing (PDGF-AA) Platelet-derived growth factor composed of two A subunits and NT-3 (PeproTech).

    Article Title: IL-17A injury to retinal ganglion cells is mediated by retinal Müller cells in diabetic retinopathy
    Article Snippet: The retinal cells were resuspended in panning buffer, filtered through a 20 μm nylon mesh, and incubated with rat anti-macrophage antibody (Abcam, Cambridge, UK). .. Contaminating microglial cells were removed by incubating the cells on an anti-rat IgG-coated dish, followed by RGC selection on dishes sequentially coated with goat anti-mouse IgG and mouse anti-Thy1.2 (clone F7D5; AbD Serotec, Oxford, UK). ..

    Article Title: Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
    Article Snippet: .. Briefly, OPCs were purified by negatively selecting with mouse anti-Thy1.2 (BioRad, Hercules, CA; Cat #MCA02R) and mouse anti-GalC (Millipore, Burlington, MA; Cat #MAB342), followed by mouse anti-O4 (O4 hybridoma supernatant) for positive selection. .. After releasing OPCs from the O4 plate by trypsinization, cells were resuspended in proliferation media containing PDGF-AA and NT-3 (PreproTech, Rocky Hill, NJ ).



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    Image Search Results


    (A) Quantification of total CD4 + and CD8 + T cells in the lungs of mice treated with anti-Thy1.2 depleting Abs (orange) or IgG control Abs (gray). Statistical significance was determined by the student’s t-test. Boxes indicate interquartile range with a line drawn at the mean; whiskers indicate the minimum and maximum values. (B) T RM content in the lungs of T cell-depleted or control mice in (A) shown in representative flow cytometry plots (left) and compiled results (right) of CD45 expression from i.v. Ab administration (CD45(IV), left) and CD69 expression (right) by CD4 + (top), and CD8 + (bottom) T cells. (C) Lung macrophage accumulation after influenza infection in T RM -depleted (blue) and control (green) mice undergoing secondary infection at 4dpi compared to uninfected counterparts shown in representative flow cytometry plots (left) and compiled results (right) of F4/80 + macrophages in the lungs. Statistical significance for (A-C) was determined by the student’s t-test. (D-E) Pearson correlation analyses between T RM and CD4 + and CD8 + T RM subsets with (D) macrophages and with (E) Arg1 + macrophages. Data are representative of two independent experiments, with n=7–10 mice per group. ****, p ≤ 0.0001; ***; p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05.

    Journal: The Journal of experimental medicine

    Article Title: Lung tissue-resident memory T cells optimize protection by IL-10 regulation of innate immunity

    doi: 10.1084/jem.20242307

    Figure Lengend Snippet: (A) Quantification of total CD4 + and CD8 + T cells in the lungs of mice treated with anti-Thy1.2 depleting Abs (orange) or IgG control Abs (gray). Statistical significance was determined by the student’s t-test. Boxes indicate interquartile range with a line drawn at the mean; whiskers indicate the minimum and maximum values. (B) T RM content in the lungs of T cell-depleted or control mice in (A) shown in representative flow cytometry plots (left) and compiled results (right) of CD45 expression from i.v. Ab administration (CD45(IV), left) and CD69 expression (right) by CD4 + (top), and CD8 + (bottom) T cells. (C) Lung macrophage accumulation after influenza infection in T RM -depleted (blue) and control (green) mice undergoing secondary infection at 4dpi compared to uninfected counterparts shown in representative flow cytometry plots (left) and compiled results (right) of F4/80 + macrophages in the lungs. Statistical significance for (A-C) was determined by the student’s t-test. (D-E) Pearson correlation analyses between T RM and CD4 + and CD8 + T RM subsets with (D) macrophages and with (E) Arg1 + macrophages. Data are representative of two independent experiments, with n=7–10 mice per group. ****, p ≤ 0.0001; ***; p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05.

    Article Snippet: To deplete T RM from mice, WT mice were infected with X31 influenza virus as above and 3 weeks post infection (wkpi), the resultant memory mice were administered 10μg/g of Thy1.2-depleting antibody (BioXcell; BE0066) by intraperitoneal (IP) injection four times over the course of one week.

    Techniques: Control, Flow Cytometry, Expressing, Infection