anti tab2 (Proteintech)
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anti tab2
Anti Tab2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tab2/TAB2+Polyclonal+antibody/pmc12659515-109-49-51
Average 93 stars, based on 30 article reviews
Anti Tab2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tab2/TAB2+Polyclonal+antibody/pmc12659515-109-49-51
Average 93 stars, based on 30 article reviews
anti tab2 - by Bioz Stars,
2026-10
93/100 stars
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Quantitative RT-PCR:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Expressing:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Transfection:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Over Expression:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Plasmid Preparation:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Control:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Western Blot:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Software:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Migration:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), CCK-8 Assay:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Colony Assay:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Transwell Migration Assay:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Co-Culture Assay:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Knockdown:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Activation Assay:Article Title: Hsa-miR-423-5p selectively loaded in hypoxic exosomes reduces the sensitivity of normoxic hepatocellular carcinoma to sorafenib via autophagy Article Snippet: The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).The proteins were then visualized using Clarity Western ECL Substrate (Bio-Rad, USA).. The following primary antibodies were used: anti-β-actin (20536-1-AP, Proteintech, Wuhan, China), anti-TSG101 (ab125011, Abcam, Cambridge, UK), anti-CD81 (ab109201, Abcam, Cambridge, UK), anti-calnexin (ab22595, Abcam, Cambridge, UK), anti-LC3B (ET1701-65, Huabio, Hangzhou, China), anti-SQSTM1 (#8025, Cell Signaling Technology, USA), anti-HIF1α (#36169, Cell Signaling Technology, USA), anti-HNRNPA1 (ET1704-52, Huabio, Hangzhou, China), and Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF ‐ κB Pathway Activation in Cervical Cancer Article Snippet: The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non‐fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: TAB2 Promotes Immune Escape and Chemoresistance Through NF-κB Pathway Activation in Cervical Cancer. Article Snippet: The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.The membranes were blocked with 5% non- fat milk in TBST for 1 h at room temperature and then incubated with primary antibodies overnight at 4°C.. The following primary antibodies were used: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals Article Snippet: The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubated with skim milk powder dissolved with Tris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: The antisense lncRNA of TAB2 that prevents oxidative stress to enhance the follicular growth in mammals. Article Snippet: The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.The PVDF membranes were incubatedwith skimmilk powder dissolvedwithTris-buffered saline-Tween (TBST) for 2 h and then incubated with diluted primary antibodies at 4°C overnight.. The information of primary antibody were as follows: Article Title: Promotion of nonalcoholic steatohepatitis by RNA N 6 -methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phospho-JNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), anti-c-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), Article Title: Promotion of nonalcoholic steatohepatitis by RNA N6-methyladenosine reader IGF2BP2 in mice Article Snippet: All protein samples were equally subjected to 10% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes by electrophoresis, incubated with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore).with primary and secondary antibodies, and finally visualized by a chemiluminescence detection kit (Millipore). ... The following primary antibodies were used: anti-phospho-P65 at 1:1000 (catalog 3033, Cell Signaling Technology), anti-total P65 at 1:1000 (catalog 8242, Cell Signaling Technology), anti-phosphoJNK at 1:1000 (catalog 9251, Cell Signaling Technology), anti-total JNK at 1:1000 (catalog 9252, Cell Signaling Technology), antic-FLIP at 1:500 (catalog sc-5276, Santa Cruz), anti-Caspase 3 at 1:1000 (catalog 9661, Cell Signaling Technology), anti-IGF2BP2 at 1:1000 (catalog 11601-1-AP, Proteintech Group), |
