anti syntaxin 4 (R&D Systems)
Structured Review
Anti Syntaxin 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+syntaxin+4/Human+Syntaxin+4+Antibody/bio_rxiv__64898__2026__01__21__700773-234-15-17
Average 91 stars, based on 2 article reviews
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Incubation:Article Title: Tumor-Derived Extracellular Vesicles Breach the Intact Blood-Brain Barrier via Transcytosis Article Snippet: Cells were co-incubated with TdTom-Br-EVs (8 × 10 9 particles/well) and 70 kDa FITC-dextran (0.5 mg/mL), Alexa Fluor 647-conjugated transferrin (50 μ g/mL, Thermo Fisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T23366","term_id":"511388","term_text":"T23366"}} T23366 ), or DQ ovalbumin (200 μ g/mL, Thermo Fisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"D12053","term_id":"2148854","term_text":"D12053"}} D12053 ) for 30 min. .. Subsequently, cells were washed 4 times with PBS and fixed with 4% formaldehyde for 10 min. For evaluation of colocalization with EEA1 and caveolin-1 (15 min incubation) or SNARE complexes (30 min incubation), cells were incubated with TdTom-Br-EVs (8 × 10 9 particles/well) and then washed and fixed for staining with anti-EEA1 (1:100, Cell Signaling Technologies, cat. no. 3288), anti-caveolin-1 (1:100, Cell Signaling Technologies, cat. no. 3267), anti-VAMP-3 (1:100, Abcam, cat. no. ab200657), anti-TGN46 (1:50, Abcam, cat. no. ab2809), anti-VAMP-7 (1:100, Cell Signaling Technologies, cat. no. 13786), Article Title: Dual Ligand Cooperation at the Plasma Membrane Drives Transport of Engineered Small Extracellular Vesicles Across Brain Endothelial Cells Article Snippet: Image analysis was processed in Imaris 10.1 software (Oxford Instruments, U.K.). .. After blocking for 30 min with 3% (w/v) BSA solution, the cells were stained with Staining:Article Title: Tumor-Derived Extracellular Vesicles Breach the Intact Blood-Brain Barrier via Transcytosis Article Snippet: Cells were co-incubated with TdTom-Br-EVs (8 × 10 9 particles/well) and 70 kDa FITC-dextran (0.5 mg/mL), Alexa Fluor 647-conjugated transferrin (50 μ g/mL, Thermo Fisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"T23366","term_id":"511388","term_text":"T23366"}} T23366 ), or DQ ovalbumin (200 μ g/mL, Thermo Fisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"D12053","term_id":"2148854","term_text":"D12053"}} D12053 ) for 30 min. .. Subsequently, cells were washed 4 times with PBS and fixed with 4% formaldehyde for 10 min. For evaluation of colocalization with EEA1 and caveolin-1 (15 min incubation) or SNARE complexes (30 min incubation), cells were incubated with TdTom-Br-EVs (8 × 10 9 particles/well) and then washed and fixed for staining with anti-EEA1 (1:100, Cell Signaling Technologies, cat. no. 3288), anti-caveolin-1 (1:100, Cell Signaling Technologies, cat. no. 3267), anti-VAMP-3 (1:100, Abcam, cat. no. ab200657), anti-TGN46 (1:50, Abcam, cat. no. ab2809), anti-VAMP-7 (1:100, Cell Signaling Technologies, cat. no. 13786), Article Title: Dual Ligand Cooperation at the Plasma Membrane Drives Transport of Engineered Small Extracellular Vesicles Across Brain Endothelial Cells Article Snippet: Image analysis was processed in Imaris 10.1 software (Oxford Instruments, U.K.). .. After blocking for 30 min with 3% (w/v) BSA solution, the cells were stained with Blocking Assay:Article Title: Dual Ligand Cooperation at the Plasma Membrane Drives Transport of Engineered Small Extracellular Vesicles Across Brain Endothelial Cells Article Snippet: Image analysis was processed in Imaris 10.1 software (Oxford Instruments, U.K.). .. After blocking for 30 min with 3% (w/v) BSA solution, the cells were stained with |

