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anti stat6  (Proteintech)


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    Structured Review

    Proteintech anti stat6
    Anti Stat6, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stat6/STAT6+Antibody/pm42785223-420-58-59
    Average 95 stars, based on 73 article reviews
    anti stat6 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: ADGRG6 Promotes Pancreatic Adenocarcinoma Progression Through the NF-κB/STAT6 Axis and Modulation of the Tumor Immune Microenvironment
    Article Snippet: .. Total protein lysates were prepared from AsPC-1 cells transfected with si-ADGRG6 or si-NC for 48 h. Blots were probed using anti-ADGRG6 (1:1000, Proteintech, Rosemont, IL, USA), anti-phospho-NF-κB p65 (Ser468) (1:1000, Proteintech), anti-NF-κB p65 (1:1000, Proteintech), anti-STAT6 (1:1000, Proteintech), anti-phospho-STAT6 (Tyr641) (1:1000, Proteintech), anti-GATA3 (1:1000, Proteintech), and anti-GAPDH (1:3000, Proteintech) antibodies. .. Bands were visualized using Enhanced Chemiluminescence (ECL, Meilunbio, Dalian, China) and imaged with a ChemiDoc TM ; Imaging System (Bio-Rad, Hercules, CA, USA).

    Western Blot:

    Article Title: Bone Marrow Mesenchymal Stem Cell‐Derived Exosomes Promote <scp>M2</scp> Polarization and Protect Against Acute Lung Injury
    Article Snippet: The lysates were then subjected to immunoprecipitation using 4 μg of anti- HA antibody or normal rabbit IgG (ab9100/ab125938, Abcam), together with protein A- Sepharose (Santa Cruz Biotechnology, Texas, USA). .. The immune complexes were washed and analyzed by immunoblotting with anti- STAT6 (1:800) and anti- pan Acetylation (66289, Proteintech, USA) (1:500) antibodies. ..

    Article Title: Bone Marrow Mesenchymal Stem Cell‐Derived Exosomes Promote M2 Polarization and Protect Against Acute Lung Injury
    Article Snippet: The lysates were then subjected to immunoprecipitation using 4 μg of anti‐HA antibody or normal rabbit IgG (ab9100/ab125938, Abcam), together with protein A‐Sepharose (Santa Cruz Biotechnology, Texas, USA). .. The immune complexes were washed and analyzed by immunoblotting with anti‐STAT6 (1:800) and anti‐pan Acetylation (66289, Proteintech, USA) (1:500) antibodies. ..

    Saline:

    Article Title: Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were blocked with 5% non-fat milk in Tris-Buffered Saline with Tween 20 (TBST) for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: anti–β-tubulin (TA-10, ZsBio), anti–β-actin (TA-09, ZsBio), anti-CCL16 (YN1319, Immunoway), anti–ICAM-1 (10831-1-AP, Proteintech), anti-JAK2 (ET1607-35, HUABIO), anti–pJAK2 (ET1607-34, HUABIO), anti-STAT6 (51073-1-AP, Proteintech), anti–pSTAT6 (ab188080, Abcam), anti-DPP4 (DF12387, Affinity), anti-IL24 (26772-1-AP, Proteintech), anti-F4/80 (28463-1-AP, Proteintech), anti-CCR1 (DF2710, Affinity), anti-CD31 (ab124432, Abcam), anti-SMA (ZM-0003, ZsBio), anti-GST (#2625, Cell Signaling Technology), and anti-FLAG (F1804, Sigma-Aldrich). ..

    Incubation:

    Article Title: Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were blocked with 5% non-fat milk in Tris-Buffered Saline with Tween 20 (TBST) for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: anti–β-tubulin (TA-10, ZsBio), anti–β-actin (TA-09, ZsBio), anti-CCL16 (YN1319, Immunoway), anti–ICAM-1 (10831-1-AP, Proteintech), anti-JAK2 (ET1607-35, HUABIO), anti–pJAK2 (ET1607-34, HUABIO), anti-STAT6 (51073-1-AP, Proteintech), anti–pSTAT6 (ab188080, Abcam), anti-DPP4 (DF12387, Affinity), anti-IL24 (26772-1-AP, Proteintech), anti-F4/80 (28463-1-AP, Proteintech), anti-CCR1 (DF2710, Affinity), anti-CD31 (ab124432, Abcam), anti-SMA (ZM-0003, ZsBio), anti-GST (#2625, Cell Signaling Technology), and anti-FLAG (F1804, Sigma-Aldrich). ..

    Cell-Signaling:

    Article Title: Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma
    Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were blocked with 5% non-fat milk in Tris-Buffered Saline with Tween 20 (TBST) for 1 hour at room temperature, followed by overnight incubation at 4°C with the following primary antibodies: anti–β-tubulin (TA-10, ZsBio), anti–β-actin (TA-09, ZsBio), anti-CCL16 (YN1319, Immunoway), anti–ICAM-1 (10831-1-AP, Proteintech), anti-JAK2 (ET1607-35, HUABIO), anti–pJAK2 (ET1607-34, HUABIO), anti-STAT6 (51073-1-AP, Proteintech), anti–pSTAT6 (ab188080, Abcam), anti-DPP4 (DF12387, Affinity), anti-IL24 (26772-1-AP, Proteintech), anti-F4/80 (28463-1-AP, Proteintech), anti-CCR1 (DF2710, Affinity), anti-CD31 (ab124432, Abcam), anti-SMA (ZM-0003, ZsBio), anti-GST (#2625, Cell Signaling Technology), and anti-FLAG (F1804, Sigma-Aldrich). ..

    other:

    Article Title: Infrapatellar fat pad exosomes promote osteoarthritis by reprogramming macrophage polarization.
    Article Snippet: Anti-RAB27A (Abcam, ab55667), anti-CD63 (Affinity, AF5117), anti-CD81 (Abcam, ab79559), anti-TSG101 (Abcam, ab133586), antiCalnexin (Abcam, ab133615), anti-iNOS (Abcam, ab178945), antiCD68 (Proteintech, 25747-1-AP), anti-CD206 (Proteintech, 18704-1- AP), anti-Arg-1 (Abcam, ab233548), anti-CD86 (ABclonal, A1199), anti-SOX9 (Abcam, ab185966), anti-CollagenII (Abcam, ab34712), antiMMP13 (Abcam, ab39012), anti-ADAMTS5 (Abcam, ab41037), IL-6 (Abcam, ab290735), anti-NLRP3 (Abcam, ab263899), IL-1β (Proteintech, 66009-1-Ig), anti-TRAF3 (Proteintech, 66310-1-Ig), TNF-α (Proteintech, 17590-1-AP), anti-STAT6 (Proteintech, 51073-1-AP), antip-STAT6 (Affinity, AF3301), anti-PPARγ (Abcam, ab45036), AntiUbiquitin (linkage-specific K63) (Abcam, ab179434), anti-NICD (Proteintech, 20687-1-AP), anti-Hes1 (Abcam, ab108937) and anti-β-actin (Immunoway, YM3028) antibodies were used.



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