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rabbit anti rat igg  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rabbit anti rat igg
    Rabbit Anti Rat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rat/Unconjugated+Rabbit+Anti-Rat+IgG+Antibody/10__22203_slash_ecm__v033a11-129-32-73
    Average 96 stars, based on 2170 article reviews
    rabbit anti rat igg - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Anti-CSF-1R therapy with combined immuno-chemotherapy coordinate an adaptive immune response to eliminate macrophage enriched triple negative breast cancers.
    Article Snippet: For IHC the following primary antibodies were utilized: anti-F4/80 (Cell Signaling Technology, 70076; 1:500), anti-S100A8 (R&D Systems, MAB3059; 1:5000), anti-CD8α (Cell Signaling Technology, 98941; 1:500) and anti-CD4 (Abcam, ab183685 1:400). .. Biotin-conjugated secondary antibodies, anti-rabbit (Vector Laboratories, PI-1000-1) or anti-rat (Vector Laboratories, PI-9400-1) were incubated for 1h at room temperature. .. VECTASTAIN Elite ABC HRP Reagent (Vector Laboratories, PK7100) was used to amplify signal according to the manufacture’s protocol and developed with ImmPACT DAB peroxidase substrate (Vector Laboratories, sk-4105).

    Article Title: Increased Meflin expression in cancer-associated fibroblasts restrains tumor cell proliferation and shapes vessel-rich stroma in triple-negative breast cancer.
    Article Snippet: .. Next, the slides were 265 incubated with the secondary antibody using the ImmPRESS HRP Goat Anti-Mouse (#MP-266 7452, Vector) or Anti-Rat (#MP-7404, Vector) IgG Polymer Detection Kit for 15 min at RT. ..

    Article Title: Anti-CSF-1R therapy with combined immuno-chemotherapy coordinate an adaptive immune response to eliminate macrophage enriched triple negative breast cancers
    Article Snippet: Sections were then incubated in the appropriate blocking buffer containing 3% BSA (Sigma-Aldrich, A7906) and 5% goat serum (Sigma-Aldrich, G9023) in PBS for 1 h at room temperature and incubated in the respective primary antibodies overnight at 4 o C. For IHC the following primary antibodies were utilized: anti-F4/80 (Cell Signaling Technology, 70076; 1:500), anti-S100A8 (R&D Systems, MAB3059; 1:5000), anti-CD8α (Cell Signaling Technology, 98941; 1:500) and anti-CD4 (Abcam, ab183685 1:400). .. Biotin-conjugated secondary antibodies, anti-rabbit (Vector Laboratories, PI-1000-1) or anti-rat (Vector Laboratories, PI-9400-1) were incubated for 1 h at room temperature. .. VECTASTAIN Elite ABC HRP Reagent (Vector Laboratories, PK7100) was used to amplify signal according to the manufacture’s protocol and developed with ImmPACT DAB peroxidase substrate (Vector Laboratories, sk-4105).

    Article Title: Western diet suppresses canonical intestinal stem cells and reprograms c-Kit⁺ reserve stem cells via proinflammatory dysbiosis
    Article Snippet: Sections were blocked with 4% goat serum (GS, Thermo Fisher Scientific, 16210064) in PBST for 1.5 hours at room temperature, then incubated overnight at 4°C with the following primary antibodies: β-catenin (Cell Signaling Technology, 8814); YAP (Cell Signaling Technology, 14074T); and Ki67 (BioLegend, 652402). .. Sections were washed three times in 2% GS in PBST, then incubated for 1.5 hours at room temperature with the following biotinylated secondary antibodies diluted in 2% GS in PBST: Anti-Rabbit (Vector Laboratories, BA-1000-1.5), and Anti-Rat (Vector Laboratories, BA-1000-1.5). .. Following three washes in 1% GS in PBST, sections were incubated with avidin-biotin complex (ABC) solution (Vector Laboratories, PK-6100) for 1 hour at room temperature in the dark.

    Article Title: Increased Meflin expression in cancer-associated fibroblasts restrains tumor cell proliferation and shapes vessel-rich stroma in triple-negative breast cancer.
    Article Snippet: .. Next, the slides were incubated with the secondary antibody using an HRP 152 Goat Anti-Mouse (#MP-7452, Vector Laboratories, Newark, CA, USA), Anti-Rabbit (#MP-153 7451, Vector), or Anti-Rat (#MP-7404, Vector) IgG Polymer Detection Kit for 15 min at RT. .. 154 Reaction products were visualized using diaminobenzidine (#K3468, Dako / Agilent 155 Technologies, Santa Clara, CA, USA).

    Polymer:

    Article Title: Increased Meflin expression in cancer-associated fibroblasts restrains tumor cell proliferation and shapes vessel-rich stroma in triple-negative breast cancer.
    Article Snippet: .. Next, the slides were 265 incubated with the secondary antibody using the ImmPRESS HRP Goat Anti-Mouse (#MP-266 7452, Vector) or Anti-Rat (#MP-7404, Vector) IgG Polymer Detection Kit for 15 min at RT. ..

    Article Title: Increased Meflin expression in cancer-associated fibroblasts restrains tumor cell proliferation and shapes vessel-rich stroma in triple-negative breast cancer.
    Article Snippet: .. Next, the slides were incubated with the secondary antibody using an HRP 152 Goat Anti-Mouse (#MP-7452, Vector Laboratories, Newark, CA, USA), Anti-Rabbit (#MP-153 7451, Vector), or Anti-Rat (#MP-7404, Vector) IgG Polymer Detection Kit for 15 min at RT. .. 154 Reaction products were visualized using diaminobenzidine (#K3468, Dako / Agilent 155 Technologies, Santa Clara, CA, USA).

    Immunohistochemistry:

    Article Title: Inactivation of CDKN2AARF Promotes p53-Independent Remodeling of the PDAC Tumor Microenvironment
    Article Snippet: The CDKN2A locus, which is frequently deleted in pancreatic ductal adenocarcinoma (PDAC), encodes two tumor suppressors, ARF and INK4A, that may influence tumorigenesis through distinct mechanisms.. Distinguishing their individual contributions to cancer could help improve the understanding of PDAC pathogenesis and potentially uncover targetable vulnerabilities.. Moreover, while ARF is known to enhance p53 function, defining its p53independent activities could elucidate new processes that drive PDAC development.



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    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

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    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

    Antibodies in this study.

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    Figure Lengend Snippet: Antibodies in this study.

    Article Snippet: HRP‐conjugated Goat anti‐Rat IgG (H + L) , AS028 , WB: 1:7000 IHC: 1:1000 , Abclonal.

    Techniques:

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    doi: 10.1101/2025.03.28.646066

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rat anti-cFos , Synaptic Systems , RRID: AB_2864765; Cat# 226 017.

    Techniques: Virus, Recombinant, Software