Cell-Signaling:Article Title: STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation
Article Snippet: .. The antibodies used were as follows: anti-STK39 (Abcam and ABclonal), anti-phospho-IRF3 (Ser396) (Cell Signaling Technology), anti-phospho-TBK1 (Ser172) (Cell Signaling Technology), anti-phospho-JNK (Thr183/Tyr185) (Cell Signaling Technology), anti-phospho-AKT (Ser473) (Cell Signaling Technology), anti-phospho-p65 (Ser536) (Cell Signaling Technology), anti-p65(Cell Signaling Technology), anti-AKT (Cell Signaling Technology), anti-IRF3 (Proteintech), anti-Flag (Proteintech), anti-HA (Proteintech), anti-PPP2R1A (Proteintech), anti-PPP2CA (Proteintech), anti-GST (Proteintech), anti-ubiquitin (Proteintech), anti-DCAF1 (Proteintech), anti-GAPDH (Proteintech), anti-TBK1 (ABclonal), anti-JNK (ABclonal), anti-K48-linkage specific Ubiquitin (ABclonal), goat anti-rabbit IgG(H + L) HRP secondary antibody (Bioworld), goat anti-mouse IgG(H + L) HRP secondary antibody (Bioworld), Zombie NIR Fixable Viability Kit (BioLegend), anti-mouse CD16/32 (BioLegend), APC anti-mouse CD45 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD45 (BioLegend), FITC anti-mouse CD3 (BioLegend), PE anti-mouse CD8 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD4 (BioLegend), APC anti-mouse CD11c (BioLegend), PE anti-mouse I-A/I-E (MHC class II) (BioLegend), APC anti-mouse F4/80 (BioLegend), FITC anti-mouse/human CD11b (BioLegend). .. STK39 inhibitors closantel and rafoxanide, SP600125, PDTC ammonium, mithramycin A, stattic, fludarabine, cycloheximide (CHX), MG-132, and 3-methyladenine (3-MA) were obtained from MedChemExpress.
Ubiquitin Proteomics:Article Title: STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation
Article Snippet: .. The antibodies used were as follows: anti-STK39 (Abcam and ABclonal), anti-phospho-IRF3 (Ser396) (Cell Signaling Technology), anti-phospho-TBK1 (Ser172) (Cell Signaling Technology), anti-phospho-JNK (Thr183/Tyr185) (Cell Signaling Technology), anti-phospho-AKT (Ser473) (Cell Signaling Technology), anti-phospho-p65 (Ser536) (Cell Signaling Technology), anti-p65(Cell Signaling Technology), anti-AKT (Cell Signaling Technology), anti-IRF3 (Proteintech), anti-Flag (Proteintech), anti-HA (Proteintech), anti-PPP2R1A (Proteintech), anti-PPP2CA (Proteintech), anti-GST (Proteintech), anti-ubiquitin (Proteintech), anti-DCAF1 (Proteintech), anti-GAPDH (Proteintech), anti-TBK1 (ABclonal), anti-JNK (ABclonal), anti-K48-linkage specific Ubiquitin (ABclonal), goat anti-rabbit IgG(H + L) HRP secondary antibody (Bioworld), goat anti-mouse IgG(H + L) HRP secondary antibody (Bioworld), Zombie NIR Fixable Viability Kit (BioLegend), anti-mouse CD16/32 (BioLegend), APC anti-mouse CD45 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD45 (BioLegend), FITC anti-mouse CD3 (BioLegend), PE anti-mouse CD8 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD4 (BioLegend), APC anti-mouse CD11c (BioLegend), PE anti-mouse I-A/I-E (MHC class II) (BioLegend), APC anti-mouse F4/80 (BioLegend), FITC anti-mouse/human CD11b (BioLegend). .. STK39 inhibitors closantel and rafoxanide, SP600125, PDTC ammonium, mithramycin A, stattic, fludarabine, cycloheximide (CHX), MG-132, and 3-methyladenine (3-MA) were obtained from MedChemExpress.
Immunopeptidomics:Article Title: STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation
Article Snippet: .. The antibodies used were as follows: anti-STK39 (Abcam and ABclonal), anti-phospho-IRF3 (Ser396) (Cell Signaling Technology), anti-phospho-TBK1 (Ser172) (Cell Signaling Technology), anti-phospho-JNK (Thr183/Tyr185) (Cell Signaling Technology), anti-phospho-AKT (Ser473) (Cell Signaling Technology), anti-phospho-p65 (Ser536) (Cell Signaling Technology), anti-p65(Cell Signaling Technology), anti-AKT (Cell Signaling Technology), anti-IRF3 (Proteintech), anti-Flag (Proteintech), anti-HA (Proteintech), anti-PPP2R1A (Proteintech), anti-PPP2CA (Proteintech), anti-GST (Proteintech), anti-ubiquitin (Proteintech), anti-DCAF1 (Proteintech), anti-GAPDH (Proteintech), anti-TBK1 (ABclonal), anti-JNK (ABclonal), anti-K48-linkage specific Ubiquitin (ABclonal), goat anti-rabbit IgG(H + L) HRP secondary antibody (Bioworld), goat anti-mouse IgG(H + L) HRP secondary antibody (Bioworld), Zombie NIR Fixable Viability Kit (BioLegend), anti-mouse CD16/32 (BioLegend), APC anti-mouse CD45 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD45 (BioLegend), FITC anti-mouse CD3 (BioLegend), PE anti-mouse CD8 (BioLegend), PerCP/Cyanine5.5 anti-mouse CD4 (BioLegend), APC anti-mouse CD11c (BioLegend), PE anti-mouse I-A/I-E (MHC class II) (BioLegend), APC anti-mouse F4/80 (BioLegend), FITC anti-mouse/human CD11b (BioLegend). .. STK39 inhibitors closantel and rafoxanide, SP600125, PDTC ammonium, mithramycin A, stattic, fludarabine, cycloheximide (CHX), MG-132, and 3-methyladenine (3-MA) were obtained from MedChemExpress.
Immunofluorescence:Article Title: Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor
Article Snippet: The human PPP2CA plasmid is from addgene and is cloned into a pBABE zeo vector . pMet7-Flag-empty vector was used as mock DNA where appropriate, to reach the same amount of transfected DNA per well for all set-ups. .. Anti-GR (rabbit, H300, Santa cruz Biotechnology), anti-PPP2CA (rabbit, Proteintech), anti-Phosphorylated S211 Glucocorticoid receptor (rabbit, cell signaling technology), anti-STRN3 [1) N-20, goat polyclonal, sc:16853, Santa cruz Biotechnology, used in immunofluorescence experiments, 2) S68, mouse monoclonal, MAI-46461, Thermo Scientific, 3) mouse monoclonal, 05-1115, Millipore] (1 and 2 successfully detect over-expressed STRN3 in western blots), anti-Flag (mouse, Sigma), anti-Flag (rabbit, Sigma), anti-Lamin A/C (mouse, Cell signaling technology), anti-GAPDH (rabbit, Abcam), anti-PARP-1 (H-250, Santa cruz Biotechnology), horseradish peroxidase-conjugated anti-mouse and anti-rabbit from Jackson Immunoresearch laboratories, Pierce donkey anti-rabbit secondary antibody dylight 800 or 680 and Pierce donkey anti-mouse secondary antibody dylight 680 (Thermo Scientific). ..
Western Blot:Article Title: Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor
Article Snippet: The human PPP2CA plasmid is from addgene and is cloned into a pBABE zeo vector . pMet7-Flag-empty vector was used as mock DNA where appropriate, to reach the same amount of transfected DNA per well for all set-ups. .. Anti-GR (rabbit, H300, Santa cruz Biotechnology), anti-PPP2CA (rabbit, Proteintech), anti-Phosphorylated S211 Glucocorticoid receptor (rabbit, cell signaling technology), anti-STRN3 [1) N-20, goat polyclonal, sc:16853, Santa cruz Biotechnology, used in immunofluorescence experiments, 2) S68, mouse monoclonal, MAI-46461, Thermo Scientific, 3) mouse monoclonal, 05-1115, Millipore] (1 and 2 successfully detect over-expressed STRN3 in western blots), anti-Flag (mouse, Sigma), anti-Flag (rabbit, Sigma), anti-Lamin A/C (mouse, Cell signaling technology), anti-GAPDH (rabbit, Abcam), anti-PARP-1 (H-250, Santa cruz Biotechnology), horseradish peroxidase-conjugated anti-mouse and anti-rabbit from Jackson Immunoresearch laboratories, Pierce donkey anti-rabbit secondary antibody dylight 800 or 680 and Pierce donkey anti-mouse secondary antibody dylight 680 (Thermo Scientific). ..
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