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mouse anti odc1 mabs  (Proteintech)


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    Structured Review

    Proteintech mouse anti odc1 mabs
    Mouse Anti Odc1 Mabs, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+odc/ODC1+Antibody/pm42543071-48-0-7
    Average 94 stars, based on 42 article reviews
    mouse anti odc1 mabs - by Bioz Stars, 2026-11
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Targeting RNA editing of antizyme inhibitor 1 : A potential oligonucleotide-based antisense therapy for cancer
    Article Snippet: Protein lysates were prepared with Radioimmunoprecipitation assay (RIPA) buffer (Sigma) supplemented with 1 cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) and quantified by Bradford assay (Bio-Rad). .. Protein lysates were then separated by 8%–10% SDSPAGE, followed by incubation with primary antibodies (1:1,000 dilution) overnight at 4 C and incubation with secondary antibodies (1:10,000 dilution) at room temperature for 1 h. Primary antibodies used are anti-ADAR1 (Abcam, ab88574), anti-AZIN1 (Proteintech, 11548-1-AP), anti-b-actin (1:10,000; Santa Cruz Biotechnology, sc47778), anti-Flag-horseradish peroxidase (1:10,000; Sigma-Aldrich, A8592), anti-ODC (Proteintech, 28728-1-AP), and anti-CCND1 (Proteintech, 60186-1-Ig). ..

    Software:

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.
    Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    Microscopy:

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.
    Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    Staining:

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.
    Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes
    Article Snippet: For β-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). β-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously. .. For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    Immunofluorescence:

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.
    Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes
    Article Snippet: For β-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). β-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously. .. For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: anti-ODC (1:300; 28728-1-AP, Proteintech) and anti-insulin antibody (IR002, Dako). .. Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).

    other:

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes
    Article Snippet: Anti-ODC , ProteinTech , Cat28728-1-AP:RRID: AB_2881200.

    Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.
    Article Snippet: Alexa Fluor antibodies were used as secondary antibodies (Invitrogen).



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    Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.

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    Figure Lengend Snippet: Differences in polyamine metabolism in the colon between NX piglets and DLY piglets. ( A ) Concentration of polyamines (putrescine, spermidine, and spermine) in colonic contents; ( B ) polyamine concentration in the colon; ( C ) ODC 1 protein expression in the colon; ( D ) ODC enzyme activity in the colon; ( E ) amino acid transport carrier mRNA expression level in the colon. ODC 1: ornithine decarboxylase 1; ODC: ornithine decarboxylase. DLY: Duroc × Landrace × Yorkshire; NX: Ningxiang. * p < 0.05, ** p < 0.01.

    Article Snippet: The membranes were blocked with 5% skimmed milk for 2 h at room temperature (RT), washed in TBST buffer, and incubated overnight with primary antibodies at 4 °C (ODC1, 1:800, cat. #bs-1294R, Bioss, Beijing, China; occludin, 1:1000, cat. #27260-1-AP, Proteintech, Inc., Chicago, IL, USA), followed by secondary antibodies (cat. #A21020, anti-rabbit, 1 μg/L, Proteintech, Inc., Chicago, IL, USA).

    Techniques: Concentration Assay, Expressing, Activity Assay