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ltl  (Vector Laboratories)


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    Structured Review

    Vector Laboratories ltl
    Ltl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 410 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ltl/Biotinylated+Lotus+Tetragonolobus+Lectin+(LTL)/pmc13130776-5-0-2
    Average 96 stars, based on 410 article reviews
    ltl - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Melatonin attenuates kidney injury by alleviating lysosomal damage in diabetic kidney disease.
    Article Snippet: .. Next, the cells and tissues were incubated overnight with the following antibodies at a 1:150 dilution: anti-HO1, anti-NRF2, anti-LRP-1 (WL03211; Wanleibio), anti-TFEB (13372-1-AP; Proteintech), anti-LAMP1 (WL02419; Wanleibio), anti-GAL3 (60207-1; Proteintech), and anti-LTL (FL-1321-2; Vector, Los Angeles, USA). ..

    Article Title: Melatonin attenuates kidney injury by alleviating lysosomal damage in diabetic kidney disease
    Article Snippet: .. Next, cells and tissues were incubated overnight with the following antibodies at a 1:150 dilution: anti-HO1, anti-NRF2, anti-LRP-1 (WL03211, Wanleibio, China), anti-TFEB (13372-1-AP, Proteintech, China), anti-LAMP1 (WL02419, Wanleibio, China), anti-GAL3 (60207-1, Proteintech, China), and anti-LTL (FL-1321-2, Vector, USA). ..

    Article Title: Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion.
    Article Snippet: .. After the slides were washed in PBST buffer, they were blocked in 10 % goat serum for at least 30 min. Rabbit polyclonal anti-HMGB1 (1:300, Abcam, UK), rabbit polyclonal anti-ACSL4 (1:200, Abcam, UK), anti-LTL (1:100, Vector Laboratories, USA), rabbit polyclonal anti-Ly6G (1:100, Abcam, UK), anti-4Hydroxynonenal (1:200, Abcam), goat polyclonal anti-KIM-1 (1:800; R&D system, USA) and rabbit polyclonal anti-KSP (1:100, Proteintech, China) primary antibodies were added and incubated at 4 ◦C overnight and subsequently visualized with HRP-conjugated secondary antibodies for IHC and fluorescently labeled secondary antibodies for immunofluorescence (IF). ..

    Article Title: Melatonin attenuates kidney injury by alleviating lysosomal damage in diabetic kidney disease
    Article Snippet: .. Next, the cells and tissues were incubated overnight with the following antibodies at a 1:150 dilution: anti-HO1, anti-NRF2, anti-LRP-1 (WL03211; Wanleibio), anti-TFEB (13372-1-AP; Proteintech), anti-LAMP1 (WL02419; Wanleibio), anti-GAL3 (60207-1; Proteintech), and anti-LTL (FL-1321-2; Vector, Los Angeles, USA). ..

    other:

    Article Title: Microenvironment optimization enables kidney organoid longevity with epithelial-endothelial joint basement membrane formation
    Article Snippet: Confocal images were acquired using an Olympus IX83 microscope equipped with a disk spinning unit (DSU, Evident) and cellSense software.

    Immunohistochemistry:

    Article Title: Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion.
    Article Snippet: .. After the slides were washed in PBST buffer, they were blocked in 10 % goat serum for at least 30 min. Rabbit polyclonal anti-HMGB1 (1:300, Abcam, UK), rabbit polyclonal anti-ACSL4 (1:200, Abcam, UK), anti-LTL (1:100, Vector Laboratories, USA), rabbit polyclonal anti-Ly6G (1:100, Abcam, UK), anti-4Hydroxynonenal (1:200, Abcam), goat polyclonal anti-KIM-1 (1:800; R&D system, USA) and rabbit polyclonal anti-KSP (1:100, Proteintech, China) primary antibodies were added and incubated at 4 ◦C overnight and subsequently visualized with HRP-conjugated secondary antibodies for IHC and fluorescently labeled secondary antibodies for immunofluorescence (IF). ..

    Labeling:

    Article Title: Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion.
    Article Snippet: .. After the slides were washed in PBST buffer, they were blocked in 10 % goat serum for at least 30 min. Rabbit polyclonal anti-HMGB1 (1:300, Abcam, UK), rabbit polyclonal anti-ACSL4 (1:200, Abcam, UK), anti-LTL (1:100, Vector Laboratories, USA), rabbit polyclonal anti-Ly6G (1:100, Abcam, UK), anti-4Hydroxynonenal (1:200, Abcam), goat polyclonal anti-KIM-1 (1:800; R&D system, USA) and rabbit polyclonal anti-KSP (1:100, Proteintech, China) primary antibodies were added and incubated at 4 ◦C overnight and subsequently visualized with HRP-conjugated secondary antibodies for IHC and fluorescently labeled secondary antibodies for immunofluorescence (IF). ..

    Immunofluorescence:

    Article Title: Cytoplasmic HMGB1 induces renal tubular ferroptosis after ischemia/reperfusion.
    Article Snippet: .. After the slides were washed in PBST buffer, they were blocked in 10 % goat serum for at least 30 min. Rabbit polyclonal anti-HMGB1 (1:300, Abcam, UK), rabbit polyclonal anti-ACSL4 (1:200, Abcam, UK), anti-LTL (1:100, Vector Laboratories, USA), rabbit polyclonal anti-Ly6G (1:100, Abcam, UK), anti-4Hydroxynonenal (1:200, Abcam), goat polyclonal anti-KIM-1 (1:800; R&D system, USA) and rabbit polyclonal anti-KSP (1:100, Proteintech, China) primary antibodies were added and incubated at 4 ◦C overnight and subsequently visualized with HRP-conjugated secondary antibodies for IHC and fluorescently labeled secondary antibodies for immunofluorescence (IF). ..



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    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for <t>podocytes,</t> <t>PDGFRβ</t> for mesangial cells, <t>LTL</t> for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001
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    Vector Laboratories biotinylated ltl
    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for <t>podocytes,</t> <t>PDGFRβ</t> for mesangial cells, <t>LTL</t> for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001
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    Image Search Results


    SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for podocytes, PDGFRβ for mesangial cells, LTL for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: An oral nanocombinatorial agent exhibits pleiotropic improvement in diabetic nephropathy via modulation of the SCAP/SREBPs pathway

    doi: 10.1186/s12951-026-04301-9

    Figure Lengend Snippet: SCAP/SREBPs upregulation induces lipid deposition and inflammation in DN. ( A ) Multispecies conjoint analysis of conserved biological process in DN. ( B ) Schematic of SCAP/SREBPs as integrators of lipid metabolic and inflammatory signaling pathways. ( C , D ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( C ) and tubules ( D ) of DN patients ( n = 6). Scale bars = 20–50 μm. ( E ) Representative immunohistochemical staining of CD68 in kidney of DN patients ( n = 6). Scale bar = 50 μm. ( F ) Heat map of Scap, Srebf1, Srebf2 and their downstream target gene expression in DN mice ( n = 6) from GSE200322 . ( G ) Correlation analysis between Scap, Srebf1, Srebf2 and renal inflammation, fibrosis, glomerular/tubular injury markers in DN mice ( n = 6) from GSE200322 . ( H ) Scheme of the STZ/HFD-induced T2DN mouse model. ( I ) Representative Oil Red O staining of renal glomeruli and tubules in DN mice ( n = 6). Scale bar = 10–50 μm. ( J , K ) Representative immunohistochemical staining for SREBP1, SREBP2, and SCAP in renal glomeruli ( J ) and tubules ( K ) of DN mice ( n = 6). Scale bar = 10–50 μm. ( L ) Western blot analysis of SCAP, p-SREBP1, n-SREBP1, p-SREBP2, and n-SREBP2 in DN mouse renal cortex ( n = 6). ( M ) Representative immunohistochemical staining of F4/80 in kidneys of DN mice ( n = 6). Scale bar = 50 μm. ( N ) Relative mRNA levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) in the DN mouse renal cortex ( n = 6). ( O ) Representative immunofluorescence staining of SCAP with cell markers (synaptopodin for podocytes, PDGFRβ for mesangial cells, LTL for proximal tubules) in DN mice. Scale bar = 10–20 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Anti-PDGFRβ antibody (Catalog No. 3169) was purchased from CST (MA, USA), anti-LTL antibody (Catalog No. FL-1321-2) was purchased from vector laboratories (San Francisco, USA), anti-synaptopodin (Catalog No. sc-515842) antibody was purchased from Santa Cruz (Texas, USA), anti-Zo-1 antibody (Catalog No. TA5145) was purchased from Abmart (Shanghai, China).

    Techniques: Protein-Protein interactions, Immunohistochemical staining, Staining, Targeted Gene Expression, Western Blot, Immunofluorescence

    Therapeutic efficacy of oral CLNPs in DN mice. ( A ) Scheme of DN model establishment and treatment protocol, mice were administered lycorine or CLNPs solution daily via oral gavage at a dose equivalent to 15 mg/kg of lycorine. ( B-D ) UACR (B), SCr (C), and BUN (D) in DN mice after oral administration with lycorine (15 mg/kg) or CLNPs (equivalent 15 mg/kg lycorine) ( n = 6). ( E-I ) Representative H&E, PAS, and Masson staining and quantitative analysis of kidney sections from DN mice after treatments ( n = 6). Scale bar = 10, 25–50 μm. ( J-L ) Representative IHC staining and quantification of SCAP in renal glomeruli (J, K) and tubules (J, L) of DN mice after treatments ( n = 6). Scale bar = 10–25 μm. ( M ) Representative immunofluorescence co-staining of SCAP, Golgin97 and PDGFRβ in renal glomeruli of DN mice after treatments ( n = 6). Scale bar = 10 μm. ( N ) Representative immunofluorescence co-staining of SCAP, Golgin97 and LTL in renal tubules of DN mice after treatments ( n = 6). Scale bar = 25 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Nanobiotechnology

    Article Title: An oral nanocombinatorial agent exhibits pleiotropic improvement in diabetic nephropathy via modulation of the SCAP/SREBPs pathway

    doi: 10.1186/s12951-026-04301-9

    Figure Lengend Snippet: Therapeutic efficacy of oral CLNPs in DN mice. ( A ) Scheme of DN model establishment and treatment protocol, mice were administered lycorine or CLNPs solution daily via oral gavage at a dose equivalent to 15 mg/kg of lycorine. ( B-D ) UACR (B), SCr (C), and BUN (D) in DN mice after oral administration with lycorine (15 mg/kg) or CLNPs (equivalent 15 mg/kg lycorine) ( n = 6). ( E-I ) Representative H&E, PAS, and Masson staining and quantitative analysis of kidney sections from DN mice after treatments ( n = 6). Scale bar = 10, 25–50 μm. ( J-L ) Representative IHC staining and quantification of SCAP in renal glomeruli (J, K) and tubules (J, L) of DN mice after treatments ( n = 6). Scale bar = 10–25 μm. ( M ) Representative immunofluorescence co-staining of SCAP, Golgin97 and PDGFRβ in renal glomeruli of DN mice after treatments ( n = 6). Scale bar = 10 μm. ( N ) Representative immunofluorescence co-staining of SCAP, Golgin97 and LTL in renal tubules of DN mice after treatments ( n = 6). Scale bar = 25 μm. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Anti-PDGFRβ antibody (Catalog No. 3169) was purchased from CST (MA, USA), anti-LTL antibody (Catalog No. FL-1321-2) was purchased from vector laboratories (San Francisco, USA), anti-synaptopodin (Catalog No. sc-515842) antibody was purchased from Santa Cruz (Texas, USA), anti-Zo-1 antibody (Catalog No. TA5145) was purchased from Abmart (Shanghai, China).

    Techniques: Drug discovery, Staining, Immunohistochemistry, Immunofluorescence