Review



flag trap a  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Proteintech flag trap a
    Flag Trap A, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/TRAPA%2FSSR1+Antibody/pm42879516-210-25-27
    Average 96 stars, based on 693 article reviews
    flag trap a - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Mutant p53 R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
    Article Snippet: Protein–protein interaction was demonstrated using the Duolink II detection kit according to the manufacturer's guidelines (Sigma‐Aldrich). .. Briefly, cells were fixed with 4% paraformaldehyde, permeabilized, blocked, and incubated with mouse anti‐p53 (Santa Cruz Biotechnology, #sc‐126) and rabbit anti‐FLAG (Proteintech, #80010‐1‐RR; probing for FLAG‐tagged PDPK1) primary antibodies. .. Anti‐mouse PLUS and anti‐rabbit MINUS probes (Sigma‐Aldrich) were used as secondary antibodies.

    Western Blot:

    Article Title: The AcMYB15-AcWRKY24 module coordinates lignin and salicylic acid accumulation during kiwifruit resistance to bacterial canker.
    Article Snippet: Plants coordinate phenylpropanoid metabolism across different tissues to defend against 2 pathogens, but the underlying transcriptional mechanisms remain unclear.. Here, we 3 identify the kiwifruit transcription factor AcMYB15 as a regulator that lignin deposition 4 and salicylic acid (SA) accumulation, conferring bacterial canker resistance in kiwifruit 5 through a tissue-specific regulatory module during Pseudomonas syringae pv.. actinidiae 6 (Psa) infection.

    Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
    Article Snippet: .. 2 mg of protein lysates were mixed with 1 μg of anti-Flag (rabbit pAb, Cat. No. 12076-1-AP, Proteintech), anti-PRKN (Cat. No. 14060-1-AP, Proteintech), anti-METTL3 (Cat. No. 15073-1-AP, Proteintech), or isotype IgG control (Cat. No. 30000-0-AP, Proteintech) antibody overnight at 4°C, which was followed by addition of Protein A/G agarose for 3 h. Protein in the immunoprecipitates was harvested for enrichment detection of ubiquitinated METTL3, METTL3 and PRKN by immunoblot analysis. .. Evaluation of mRNA and protein stability To evaluate the impact of METTL3 or IGF2BP2 on CLDN2 mRNA stability, transfected LoVo and HCT-116 cells were maintained for 24 h in media plus actinomycin D (Act D, 50 μg/mL, Selleck).

    Cell-Signaling:

    Article Title: RSF1 functions as a reader of the H2Aub epigenetic mark to sustain the cancer stem cell transcriptional program in ovarian cancer
    Article Snippet: After purification by VAHTS DNA Clean Beads (Vazyme, #N411), libraries were sequenced on an Illumina platform in PE150 mode. .. Primary antibodies included anti-Flag (Proteintech, #20543-1-AP,1:50), anti-RSF1 (Customer-generated, anti-rat, 1:50), and anti-H2AK119ub (Cell Signaling Technology, #8240, 1:50). .. Secondary antibodies, including anti-rabbit IgG (Abcam, #Ab6702, 1:100) and anti-rat IgG (Abcam, #182018, 1:100), were used in this study.

    Control:

    Article Title: The PRKN/METTL3/CLDN2 axis promotes colorectal cancer development through epigenetic mechanisms.
    Article Snippet: .. 2 mg of protein lysates were mixed with 1 μg of anti-Flag (rabbit pAb, Cat. No. 12076-1-AP, Proteintech), anti-PRKN (Cat. No. 14060-1-AP, Proteintech), anti-METTL3 (Cat. No. 15073-1-AP, Proteintech), or isotype IgG control (Cat. No. 30000-0-AP, Proteintech) antibody overnight at 4°C, which was followed by addition of Protein A/G agarose for 3 h. Protein in the immunoprecipitates was harvested for enrichment detection of ubiquitinated METTL3, METTL3 and PRKN by immunoblot analysis. .. Evaluation of mRNA and protein stability To evaluate the impact of METTL3 or IGF2BP2 on CLDN2 mRNA stability, transfected LoVo and HCT-116 cells were maintained for 24 h in media plus actinomycin D (Act D, 50 μg/mL, Selleck).



    Similar Products

    flag tag  (MedChemExpress)
    94
    MedChemExpress flag tag
    Flag Tag, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/Flag+Tag+Antibody/pm42887777-166-10-15
    Average 94 stars, based on 1 article reviews
    flag tag - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    flag trap a  (Proteintech)
    96
    Proteintech flag trap a
    Flag Trap A, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/TRAPA%2FSSR1+Antibody/pm42879516-210-25-27
    Average 96 stars, based on 1 article reviews
    flag trap a - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    soluble taldo1 protein  (MedChemExpress)
    94
    MedChemExpress soluble taldo1 protein
    Soluble Taldo1 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/Anti-Flag+Affinity+Gel/pm42906737-130-17-29
    Average 94 stars, based on 1 article reviews
    soluble taldo1 protein - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    dykddddk tag flag antibody  (MedChemExpress)
    94
    MedChemExpress dykddddk tag flag antibody
    Dykddddk Tag Flag Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/DYKDDDDK+Tag+(FLAG)+Antibody+(YA780/pm42898062-120-5-12
    Average 94 stars, based on 1 article reviews
    dykddddk tag flag antibody - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    anti flag magnetic beads  (MedChemExpress)
    98
    MedChemExpress anti flag magnetic beads
    Anti Flag Magnetic Beads, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/Anti+(DYKDDDDK)+Flag+Magnetic+Beads/pm42899678-336-12-15
    Average 98 stars, based on 1 article reviews
    anti flag magnetic beads - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier
    rabbit anti flag  (Proteintech)
    96
    Proteintech rabbit anti flag
    Rabbit Anti Flag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/DYKDDDDK+tag+Recombinant+antibody/pm42902976-410-49-53
    Average 96 stars, based on 1 article reviews
    rabbit anti flag - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    flag tag  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc flag tag
    Flag Tag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/DYKDDDDK+Tag+Rabbit+mAb/pmc13674512-352-67-69
    Average 99 stars, based on 1 article reviews
    flag tag - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    anti flag monoclonal antibody  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc anti flag monoclonal antibody
    Anti Flag Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/DYKDDDDK+Tag+Rabbit+mAb/pm42882489-136-5-8
    Average 99 stars, based on 1 article reviews
    anti flag monoclonal antibody - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    anti flag  (MedChemExpress)
    98
    MedChemExpress anti flag
    Anti Flag, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/Anti+(DYKDDDDK)+Flag+Magnetic+Beads/pmc13669683-416-7-8
    Average 98 stars, based on 1 article reviews
    anti flag - by Bioz Stars, 2026-11
    98/100 stars
    		  Buy from Supplier
    anti flag tag antibody  (Proteintech)
    93
    Proteintech anti flag tag antibody
    RHOQ interacts with PAK1 and promotes its phosphorylation. (A) Immunoprecipitation assay for the binding of HA‐PAK1 to flag‐RHOQ, flag‐RHOQ(Q67L) and flag‐RHOQ(T23N) in Huh7 cells. Immunoprecipitation was performed using anti‐HA <t>and</t> <t>anti‐flag</t> antibodies, and immunoblotting analysis was performed using PAK1 and RHOQ antibodies. (B) Immunoprecipitation assay for the binding of flag‐RHOQ to HA‐PAK1 and HA‐del‐PAK1 in Huh7 cells. (C) Immunoblotting analysis for PAK1 and del‐PAK1 phosphorylation levels in Huh7 cells. (D) Molecular docking simulates the binding of RHOQ to PAK1; green represents RHOQ, and blue represents PAK1. Yellow dashed lines represent hydrogen bonds. HDOCK web server: http://hdock.phys.hust.edu.cn/ . (E) Immunoblotting analysis for the PAK1 downstream effectors and their phosphorylation in Huh7 cells after IPA‐3 (10 µM) treatment. (F) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. IPA‐3 concentration was 10 µM. (G) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. FTY720 concentration was 10 µM. (H) Immunoblotting analysis for ATF6 downstream effectors and their phosphorylation in Huh7 cells. (I) Significantly enriched pathways ( Q < .05) are shown. The x‐axis indicates the Gene Ratio, while the y ‐axis shows the pathway categories. Bubble size reflects the gene count in each pathway, and bubble colour denotes the padj (FDR‐adjusted p value). (J) Represented images for transwell assay of Huh7 cells. IPA‐3 concentration was 10 µM. (K) Represented images for transwell assay of Huh7 cells. FTY720 concentration was 10 µM.
    Anti Flag Tag Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag/CL488-conjugated+DYKDDDDK+Antibody/pmc13670142-127-9-12
    Average 93 stars, based on 1 article reviews
    anti flag tag antibody - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    RHOQ interacts with PAK1 and promotes its phosphorylation. (A) Immunoprecipitation assay for the binding of HA‐PAK1 to flag‐RHOQ, flag‐RHOQ(Q67L) and flag‐RHOQ(T23N) in Huh7 cells. Immunoprecipitation was performed using anti‐HA and anti‐flag antibodies, and immunoblotting analysis was performed using PAK1 and RHOQ antibodies. (B) Immunoprecipitation assay for the binding of flag‐RHOQ to HA‐PAK1 and HA‐del‐PAK1 in Huh7 cells. (C) Immunoblotting analysis for PAK1 and del‐PAK1 phosphorylation levels in Huh7 cells. (D) Molecular docking simulates the binding of RHOQ to PAK1; green represents RHOQ, and blue represents PAK1. Yellow dashed lines represent hydrogen bonds. HDOCK web server: http://hdock.phys.hust.edu.cn/ . (E) Immunoblotting analysis for the PAK1 downstream effectors and their phosphorylation in Huh7 cells after IPA‐3 (10 µM) treatment. (F) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. IPA‐3 concentration was 10 µM. (G) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. FTY720 concentration was 10 µM. (H) Immunoblotting analysis for ATF6 downstream effectors and their phosphorylation in Huh7 cells. (I) Significantly enriched pathways ( Q < .05) are shown. The x‐axis indicates the Gene Ratio, while the y ‐axis shows the pathway categories. Bubble size reflects the gene count in each pathway, and bubble colour denotes the padj (FDR‐adjusted p value). (J) Represented images for transwell assay of Huh7 cells. IPA‐3 concentration was 10 µM. (K) Represented images for transwell assay of Huh7 cells. FTY720 concentration was 10 µM.

    Journal: Clinical and Translational Medicine

    Article Title: ATF6‐mediated GTPase RHOQ promotes liver cancer progression via enhancing aerobic glycolysis through PAK1 phosphorylation

    doi: 10.1002/ctm2.70871

    Figure Lengend Snippet: RHOQ interacts with PAK1 and promotes its phosphorylation. (A) Immunoprecipitation assay for the binding of HA‐PAK1 to flag‐RHOQ, flag‐RHOQ(Q67L) and flag‐RHOQ(T23N) in Huh7 cells. Immunoprecipitation was performed using anti‐HA and anti‐flag antibodies, and immunoblotting analysis was performed using PAK1 and RHOQ antibodies. (B) Immunoprecipitation assay for the binding of flag‐RHOQ to HA‐PAK1 and HA‐del‐PAK1 in Huh7 cells. (C) Immunoblotting analysis for PAK1 and del‐PAK1 phosphorylation levels in Huh7 cells. (D) Molecular docking simulates the binding of RHOQ to PAK1; green represents RHOQ, and blue represents PAK1. Yellow dashed lines represent hydrogen bonds. HDOCK web server: http://hdock.phys.hust.edu.cn/ . (E) Immunoblotting analysis for the PAK1 downstream effectors and their phosphorylation in Huh7 cells after IPA‐3 (10 µM) treatment. (F) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. IPA‐3 concentration was 10 µM. (G) Immunoblotting analysis for RHOQ downstream effectors and their phosphorylation in Huh7 cells. FTY720 concentration was 10 µM. (H) Immunoblotting analysis for ATF6 downstream effectors and their phosphorylation in Huh7 cells. (I) Significantly enriched pathways ( Q < .05) are shown. The x‐axis indicates the Gene Ratio, while the y ‐axis shows the pathway categories. Bubble size reflects the gene count in each pathway, and bubble colour denotes the padj (FDR‐adjusted p value). (J) Represented images for transwell assay of Huh7 cells. IPA‐3 concentration was 10 µM. (K) Represented images for transwell assay of Huh7 cells. FTY720 concentration was 10 µM.

    Article Snippet: The remaining portion was incubated overnight at 4°C with anti‐flag tag antibody (Proteintech, China), anti‐HA tag antibody (Proteintech) and isotype IgG (Beyotime).

    Techniques: Phospho-proteomics, Immunoprecipitation, Binding Assay, Western Blot, Analysis, Docking Assay, Concentration Assay, Transwell Assay