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Proteintech drd2
Drd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+drd2/DRD2+Antibody/pm41611658-91-14-20
Average 94 stars, based on 72 article reviews

drd2 - by Bioz Stars, 2026-11

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Article Title: Smilax glabra Roxb. flavonoids protect against obesity-induced cardiac injury associated with the DRD2-NF-κB signaling pathway
Article Snippet: Anti-DRD2(Cat. No. 55084-1-AP), anti-IκBα(Cat. No. 10268-1-AP), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat. No.10494-1-AP) were purchased from Proteintech Co., Ltd. (Hubei, China).

Article Title: Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects.
Article Snippet: The antibodies used were as follows: anti-p-CREB (1:1000, Proteintech, China), anti-p-CaMKII (1:1000, Proteintech, China), anti-CREB (1:1000, AdipoGen, USA), anti-CaMKII (1:1000, AdipoGen, USA), anti-BDNF (1:500, Proteintech, China), anti-DRD1 (1:1000, Proteintech, China), anti-DRD2 (1:1000, Proteintech, China), and polyclonal rabbit anti-β-actin (1:1000, Proteintech, China).

Article Title: Interrogation of RNA-bound proteome with XRNAX illuminates molecular alterations in the mouse brain affected with dysmyelination.
Article Snippet: Resource

Article Title: Neuroligin 1 regulates autistic-like repetitive behavior through modulating the excitability of striatal D2 expressing medium spiny neurons
Article Snippet: The care and use of all mice72 adhered to protocols approved by the Animal Care Committee of73 Southeast University (Nanjing, China).74 75 Antibodies, reagents and viruses76 Primary antibodies include anti-NLG1 (1:2000; Cat# 129013, Synaptic77 System), anti-GluA1 (1:2000; Cat# MAB2263, Millipore), anti-GluA278 (1:2000; Cat# MAB397, Millipore), anti-NR1 (1:2000; Cat# 5704, Cell79 Signaling Technology), anti-NR2A (1:2000; Cat# 4205, Cell Signaling80 Technology), anti-NR2B (1:2000; Cat# 14544, Cell Signaling81 Technology), Anti-HA (1:2000; Cat# 51064-2-AP, Proteintech),82 anti-Drd1(1:2000; Cat# 17934-1-AP, Proteintech), anti-Drd2 (Cat#83 55084-1-AP, Proteintech), anti-PKC(1:2000, Cat# 2056, Cell Signaling84 Technology), Anti-PKC alpha (phospho T497) (1:2000, Cat# ab76016,85 Abcam), anti-GAPDH (1:4000; Cat# 60004-1-Ig, Proteintech).

Article Title: Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons.
Article Snippet: Antibodies, Reagents, and Viruses: Primary antibodies include antiNLGN1 (1:2000; Cat# 129013, Synaptic System), anti-NLGN2 (1:2000; Cat# 129203, Synaptic System), anti-NLGN3 (1:2000; Cat#129103, Synaptic System), anti-GluA1 (1:2000; Cat# MAB2263, Millipore), anti-GluA2 (1:2000; Cat# MAB397, Millipore), anti-NR1 (1:2000; Cat# 5704, Cell Signaling Technology), anti-NR2A (1:2000; Cat# 4205, Cell Signaling Technology), anti-NR2B (1:2000; Cat# 14544, Cell Signaling Technology), anti-HA (1:2000; Cat# 51064-2-AP, Proteintech), anti-Drd1(1:2000; Cat# 17934-1-AP, Proteintech), anti-Drd2 (Cat# 55084-1-AP, Proteintech), anti-PKC(1:2000, Cat# 2056, Cell Signaling Technology), anti-PKC alpha (phospho T497) (1:2000; Cat# ab76016, Abcam), anti-PKA C-α(1:2000; Cat#5842, Cell Signaling Technology), anti-phospho-PKA C (Thr197) (1:2000; Cat#5661, Cell Signaling Technology), anti-Erk1/2 (1:2000; Cat#4695, Cell Signaling Technology), anti-phospho-Erk1/2 (Thr202/Tyr204) (1:2000; Cat#4370, Cell Signaling Technology), anti-p38 MAPK (1:2000; Cat#9212, Cell Signaling Technology), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:2000; Cat#4511,Cell Signaling Technology), anti-ChAT (1:50; Cat#AB144, Sigma-Aldrich), anti-GAPDH (1:4000; Cat# 60004-1-Ig, Proteintech).

Article Title: Neuroligin 1 Regulates Autistic‐Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor‐Expressing Medium Spiny Neurons
Article Snippet: Primary antibodies include anti‐NLGN1 (1:2000; Cat# 129013, Synaptic System), anti‐NLGN2 (1:2000; Cat# 129203, Synaptic System), anti‐NLGN3 (1:2000; Cat#129103, Synaptic System), anti‐GluA1 (1:2000; Cat# MAB2263, Millipore), anti‐GluA2 (1:2000; Cat# MAB397, Millipore), anti‐NR1 (1:2000; Cat# 5704, Cell Signaling Technology), anti‐NR2A (1:2000; Cat# 4205, Cell Signaling Technology), anti‐NR2B (1:2000; Cat# 14544, Cell Signaling Technology), anti‐HA (1:2000; Cat# 51064‐2‐AP, Proteintech), anti‐Drd1(1:2000; Cat# 17934‐1‐AP, Proteintech), anti‐Drd2 (Cat# 55084‐1‐AP, Proteintech), anti‐PKC(1:2000, Cat# 2056, Cell Signaling Technology), anti‐PKC alpha (phospho T497) (1:2000; Cat# ab76016, Abcam), anti‐PKA C‐α(1:2000; Cat#5842, Cell Signaling Technology), anti‐phospho‐PKA C (Thr197) (1:2000; Cat#5661, Cell Signaling Technology), anti‐Erk1/2 (1:2000; Cat#4695, Cell Signaling Technology), anti‐phospho‐Erk1/2 (Thr202/Tyr204) (1:2000; Cat#4370, Cell Signaling Technology), anti‐p38 MAPK (1:2000; Cat#9212, Cell Signaling Technology), anti‐Phospho‐p38 MAPK (Thr180/Tyr182) (1:2000; Cat#4511,Cell Signaling Technology), anti‐ChAT (1:50; Cat#AB144, Sigma‐Aldrich), anti‐GAPDH (1:4000; Cat# 60004‐1‐Ig, Proteintech).

Incubation:

Article Title: Extracellular Vesicles Derived from FGF2-Primed Astrocytes Against Mitochondrial and Synaptic Toxicities in Parkinson’s Disease
Article Snippet: .. The membranes were blocked with 5% skim milk powder in TBST (0.1% Tween-20) for 2 h at room temperature and incubated overnight at 4°C with following antibodies: anti-Mfn2 International Journal of Nanomedicine 2025:20 https://doi.org/10.2147/IJN.S511474 4629 Powered by TCPDF (www.tcpdf.org) (1:1000, 9482, Cell Signaling Technology, USA), anti-Opa1 (1:1000, D6U6N, Cell Signaling Technology, USA), antiDrp1 (1:1000, ab184247, Abcam, UK), anti-NCAM1 (1:10,000, 14,255-1-AP, Proteintech, China), anti-PSD95 (1:800, ab18258, Abcam, UK), anti-Synaptophysin (1:50000, ab32127, Abcam, UK), anti-β-actin (1:50000, AC026, ABclonal, China), anti-CD81 (1:1000, 27,855-1-AP, Proteintech, China), anti-CD9 (1:1000, 20,597-1-AP, Proteintech, China), antiTSG101 (1:5000, 67,381-1-Ig, Proteintech, China), anti-Calnexin (1:5000, 10,427-2-AP, Proteintech, China), anti-Sox2 (1:500, 14–9811-82, Invitrogen, USA), anti-DRD1 (1:1000, 17,934-1-AP, Proteintech, China), anti-DRD2 (1:4000, 55,084-1-AP, Proteintech, China). .. After three washes with TBST, the PVDF membrane was incubated with horseradish peroxidase-conjugated goat anti-mouse/ rabbit IgG secondary antibodies (1:5000, CSA2108/CSA2115, Cohesion Bioscience, UK).

Article Title: Extracellular Vesicles Derived from FGF2-Primed Astrocytes Against Mitochondrial and Synaptic Toxicities in Parkinson’s Disease
Article Snippet: .. The membranes were blocked with 5% skim milk powder in TBST (0.1% Tween-20) for 2 h at room temperature and incubated overnight at 4°C with following antibodies: anti-Mfn2 (1:1000, 9482, Cell Signaling Technology, USA), anti-Opa1 (1:1000, D6U6N, Cell Signaling Technology, USA), anti-Drp1 (1:1000, ab184247, Abcam, UK), anti-NCAM1 (1:10,000, 14,255-1-AP, Proteintech, China), anti-PSD95 (1:800, ab18258, Abcam, UK), anti-Synaptophysin (1:50000, ab32127, Abcam, UK), anti- β -actin (1:50000, AC026, ABclonal, China), anti-CD81 (1:1000, 27,855-1-AP, Proteintech, China), anti-CD9 (1:1000, 20,597-1-AP, Proteintech, China), anti-TSG101 (1:5000, 67,381-1-Ig, Proteintech, China), anti-Calnexin (1:5000, 10,427-2-AP, Proteintech, China), anti-Sox2 (1:500, 14–9811-82, Invitrogen, USA), anti-DRD1 (1:1000, 17,934-1-AP, Proteintech, China), anti-DRD2 (1:4000, 55,084-1-AP, Proteintech, China). .. After three washes with TBST, the PVDF membrane was incubated with horseradish peroxidase-conjugated goat anti-mouse/ rabbit IgG secondary antibodies (1:5000, CSA2108/CSA2115, Cohesion Bioscience, UK).

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Chronic intrathecal is administration resulted in reduced DRD2 expression in the dentate gyrus and impaired cognitive function. ( A )–( B ) The Hind paw Mechanical and thermal pain thresholds in rats treated with is injection were markedly lower than those in sham rats. Data analyzed by two-way anova with Bonferroni correction; n = 6 rats per group. * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared to sham rats. ( C )–( E ) Representative immunoblots and quantification illustrated decreased levels of DRD2 in the hippocampus and increased levels of CGRP in the tnc after repeated dural is stimulation; n = 6 rats per group. ( F ) RT-qPCR results showed that the mRNA expression of DRD2 in the hippocampus decreased in the CM group compared with the sham group. n = 6 rats per group. ( G )–( J ) Immunofluorescence labeling of DRD2 and neuronal markers (NeuN) in hippocampal subregions CA1, CA3, and DG, with quantitative evaluation of DRD2 fluorescence intensity; n = 4 rats per group. ( K ) A higher ratio of path length in the target quadrant in the sham group compared to the CM group during the probe trial. ( L ) Representative movement trajectories from the Morris water maze test for sham and CM groups. ( M ) IS rats showed increased escape latency relative to sham rats on the third day. Two-way anova followed by Tukey’s test; n = 8 rats per group. ( N ) Rats in the CM group spent significantly less time in the target quadrant during the probe test; data represent mean ± sem; unpaired Student’s t-test; ( O ) IS rats showed fewer platform crossings during the test session relative to sham controls. n = 8 rats per group. ( P ) IS rats showed significantly more non-spatial strategies compared to sham controls. Chi-square test of independence, χ 2 = 8.727, p = 0.0031; n = 8 rats per group. ( Q ) Thermal maps illustrating the exploration behavior of sham and is rats during the novel object recognition test. ( R ) The CM group showed a lower discrimination index during the nor test. ( S ) The CM group exhibited a lower recognition ratio during the novel object recognition test. All values represent mean ± sem; unpaired Student’s t-test unless noted; n = 8 per group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared to sham group

Journal: The Journal of Headache and Pain

Article Title: Downregulation of neuronal DRD2 drives microglia synaptic pruning and results in cognitive deficits by promoting CCL2 release in a rat model of chronic migraine

doi: 10.1186/s10194-025-02229-3

Figure Lengend Snippet: Chronic intrathecal is administration resulted in reduced DRD2 expression in the dentate gyrus and impaired cognitive function. ( A )–( B ) The Hind paw Mechanical and thermal pain thresholds in rats treated with is injection were markedly lower than those in sham rats. Data analyzed by two-way anova with Bonferroni correction; n = 6 rats per group. * p < 0.05, ** p < 0.01 and *** p < 0.001 as compared to sham rats. ( C )–( E ) Representative immunoblots and quantification illustrated decreased levels of DRD2 in the hippocampus and increased levels of CGRP in the tnc after repeated dural is stimulation; n = 6 rats per group. ( F ) RT-qPCR results showed that the mRNA expression of DRD2 in the hippocampus decreased in the CM group compared with the sham group. n = 6 rats per group. ( G )–( J ) Immunofluorescence labeling of DRD2 and neuronal markers (NeuN) in hippocampal subregions CA1, CA3, and DG, with quantitative evaluation of DRD2 fluorescence intensity; n = 4 rats per group. ( K ) A higher ratio of path length in the target quadrant in the sham group compared to the CM group during the probe trial. ( L ) Representative movement trajectories from the Morris water maze test for sham and CM groups. ( M ) IS rats showed increased escape latency relative to sham rats on the third day. Two-way anova followed by Tukey’s test; n = 8 rats per group. ( N ) Rats in the CM group spent significantly less time in the target quadrant during the probe test; data represent mean ± sem; unpaired Student’s t-test; ( O ) IS rats showed fewer platform crossings during the test session relative to sham controls. n = 8 rats per group. ( P ) IS rats showed significantly more non-spatial strategies compared to sham controls. Chi-square test of independence, χ 2 = 8.727, p = 0.0031; n = 8 rats per group. ( Q ) Thermal maps illustrating the exploration behavior of sham and is rats during the novel object recognition test. ( R ) The CM group showed a lower discrimination index during the nor test. ( S ) The CM group exhibited a lower recognition ratio during the novel object recognition test. All values represent mean ± sem; unpaired Student’s t-test unless noted; n = 8 per group; * p < 0.05, ** p < 0.01, *** p < 0.001 compared to sham group

Article Snippet: DRD2 , Santa Cruz, USA , Sc-5303 , Mouse , 1:200.

Techniques: Expressing, Injection, Western Blot, Quantitative RT-PCR, Immunofluorescence, Labeling, Fluorescence

Transcriptome analysis shows increased phagocytosis and chemokine signalling activation in glial cells in the hippocampus of is rats. ( A ) Volcano plot of differentially expressed genes (DEGs) in DRD2-KO versus wt mice. (|log₂FC| ≥ 1, FDR-adjusted p < 0.05; 846 total DEGs: 665 upregulated, 181 downregulated). ( B ) KEGG enrichment analysis highlights neuroinflammation, chemokine signalling, and microglia-related pathways (phagocytosis, protein digestion) in DRD2 ko mice. ( C ) GO analysis confirms microglial activation and phagocytosis in DRD2 ko mice. ( D ) Heatmap of key genes in the phagosome pathway. n = 3 mice per group. ( E ) Representative microglia (Iba1 + , red) immunofluorescence in hippocampal dg of WT and DRD2 ko groups. The boxed region is magnified (right). Scale bars: 100 μm (left), 20 μm (right). ( F ) Sholl analysis of microglia and a concentric circle diagram of WT and DRD2 ko groups. ( G ) No significant difference was found in the quantification of microglia in the dg area of the hippocampus between the two groups of mice.12 fields of view from 4 mice per group. ( H ) and( I ) Sholl analysis of the branch length and intersections of microglia in the dg of the hippocampus in WT and DRD2 ko groups. n = 20 microglia from 4 mice per group. ( J ) The average number of PSD95 + puncta per microglial cell was quantified in WT and DRD2 KO groups. ( K ) Representative 3D-rendered maximum projection of a confocal z-stack showing Iba1 and PSD95 in the dg of WT and DRD2 KO groups. Arrowheads indicate examples of PSD95 + puncta within Iba1 + microglial processes. ( L ) Volume ratio of engulfed PSD95 to total microglial cell volume. Data are shown as mean ± sem; unpaired Student's t-test; ** p < 0.01, *** p < 0.001 versus WT, n = 18 cells from 4 mice per group

Journal: The Journal of Headache and Pain

Article Title: Downregulation of neuronal DRD2 drives microglia synaptic pruning and results in cognitive deficits by promoting CCL2 release in a rat model of chronic migraine

doi: 10.1186/s10194-025-02229-3

Figure Lengend Snippet: Transcriptome analysis shows increased phagocytosis and chemokine signalling activation in glial cells in the hippocampus of is rats. ( A ) Volcano plot of differentially expressed genes (DEGs) in DRD2-KO versus wt mice. (|log₂FC| ≥ 1, FDR-adjusted p < 0.05; 846 total DEGs: 665 upregulated, 181 downregulated). ( B ) KEGG enrichment analysis highlights neuroinflammation, chemokine signalling, and microglia-related pathways (phagocytosis, protein digestion) in DRD2 ko mice. ( C ) GO analysis confirms microglial activation and phagocytosis in DRD2 ko mice. ( D ) Heatmap of key genes in the phagosome pathway. n = 3 mice per group. ( E ) Representative microglia (Iba1 + , red) immunofluorescence in hippocampal dg of WT and DRD2 ko groups. The boxed region is magnified (right). Scale bars: 100 μm (left), 20 μm (right). ( F ) Sholl analysis of microglia and a concentric circle diagram of WT and DRD2 ko groups. ( G ) No significant difference was found in the quantification of microglia in the dg area of the hippocampus between the two groups of mice.12 fields of view from 4 mice per group. ( H ) and( I ) Sholl analysis of the branch length and intersections of microglia in the dg of the hippocampus in WT and DRD2 ko groups. n = 20 microglia from 4 mice per group. ( J ) The average number of PSD95 + puncta per microglial cell was quantified in WT and DRD2 KO groups. ( K ) Representative 3D-rendered maximum projection of a confocal z-stack showing Iba1 and PSD95 in the dg of WT and DRD2 KO groups. Arrowheads indicate examples of PSD95 + puncta within Iba1 + microglial processes. ( L ) Volume ratio of engulfed PSD95 to total microglial cell volume. Data are shown as mean ± sem; unpaired Student's t-test; ** p < 0.01, *** p < 0.001 versus WT, n = 18 cells from 4 mice per group

Article Snippet: DRD2 , Santa Cruz, USA , Sc-5303 , Mouse , 1:200.

Techniques: Activation Assay, Immunofluorescence

Modulation of DRD2 signalling alters microglial phagocytosis, synaptic density, and cognitive function in a rat model of CM ( A ) and ( B ) Western blot and quantification of PSD95 expression in the hippocampus. Quinpirole restored PSD95 levels in the dg of is rats, while sulpiride worsened it; n = 6 rats per group. ( C ) and ( D ) Representative golgi-stained images of hippocampal dendrites. Quinpirole lessened dendritic spine loss in is rats, whereas sulpiride intensified it. Scale bar: 5 μm; 20 dendrites from 4 rats. ( E ) and ( F ) Immunofluorescence co-labelling of Iba1 + (green) and CD68 + (red) microglia in the dg. White arrows point to CD68 + microglia. Quinpirole lowered CD68 expression, while sulpiride increased it; n = 12 fields from 4rats per group. ( G ) Representative 3D-rendered confocal maximum projection of Iba1 and PSD95 in the dg. Arrowheads highlight PSD95 + puncta inside microglial processes. ( H ) Quinpirole reduced, whereas sulpiride increased, the volume ratio of engulfed PSD95 to total microglia. ( I ) The number of PSD95 + puncta per microglia. Quinpirole reduced microglial phagocytosis of PSD95 in is rats; sulpiride enhanced it; n = 18 cells from 4 rats per group. ( J ) Example paths of rats in the MWM test phase. ( K ) escape latency during MWM learning. Quinpirole decreased latency in is rats. ( L ) The distance travelled in the target quadrant is relative to the total distance. ( M ) Quinpirole treatment prolonged the time spent in the target quadrant by is rats. ( N ) The number of platform crossings in quinpirole-treated rats showed an increasing trend. Quinpirole improved all these parameters in is rats. ( O ) The discrimination index of quinpirole-treated rats was higher than that of CM-treated rats in the nort. ( P ) The recognition ratio during nort. Quinpirole increased both measures in is rats; n = 8 rats per group. Data are presented as mean ± sem. * p < 0.05, ** p < 0.01, *** p < 0.001; unpaired Student’s t-test (A–I, L–P); two-way anova with Tukey’s post hoc test ( k )

Journal: The Journal of Headache and Pain

Article Title: Downregulation of neuronal DRD2 drives microglia synaptic pruning and results in cognitive deficits by promoting CCL2 release in a rat model of chronic migraine

doi: 10.1186/s10194-025-02229-3

Figure Lengend Snippet: Modulation of DRD2 signalling alters microglial phagocytosis, synaptic density, and cognitive function in a rat model of CM ( A ) and ( B ) Western blot and quantification of PSD95 expression in the hippocampus. Quinpirole restored PSD95 levels in the dg of is rats, while sulpiride worsened it; n = 6 rats per group. ( C ) and ( D ) Representative golgi-stained images of hippocampal dendrites. Quinpirole lessened dendritic spine loss in is rats, whereas sulpiride intensified it. Scale bar: 5 μm; 20 dendrites from 4 rats. ( E ) and ( F ) Immunofluorescence co-labelling of Iba1 + (green) and CD68 + (red) microglia in the dg. White arrows point to CD68 + microglia. Quinpirole lowered CD68 expression, while sulpiride increased it; n = 12 fields from 4rats per group. ( G ) Representative 3D-rendered confocal maximum projection of Iba1 and PSD95 in the dg. Arrowheads highlight PSD95 + puncta inside microglial processes. ( H ) Quinpirole reduced, whereas sulpiride increased, the volume ratio of engulfed PSD95 to total microglia. ( I ) The number of PSD95 + puncta per microglia. Quinpirole reduced microglial phagocytosis of PSD95 in is rats; sulpiride enhanced it; n = 18 cells from 4 rats per group. ( J ) Example paths of rats in the MWM test phase. ( K ) escape latency during MWM learning. Quinpirole decreased latency in is rats. ( L ) The distance travelled in the target quadrant is relative to the total distance. ( M ) Quinpirole treatment prolonged the time spent in the target quadrant by is rats. ( N ) The number of platform crossings in quinpirole-treated rats showed an increasing trend. Quinpirole improved all these parameters in is rats. ( O ) The discrimination index of quinpirole-treated rats was higher than that of CM-treated rats in the nort. ( P ) The recognition ratio during nort. Quinpirole increased both measures in is rats; n = 8 rats per group. Data are presented as mean ± sem. * p < 0.05, ** p < 0.01, *** p < 0.001; unpaired Student’s t-test (A–I, L–P); two-way anova with Tukey’s post hoc test ( k )

Article Snippet: DRD2 , Santa Cruz, USA , Sc-5303 , Mouse , 1:200.

Techniques: Western Blot, Expressing, Staining, Immunofluorescence

IS rats exhibit elevated CCL2 expression in hippocampal neurons, which DRD2 regulates. ( A ) Heat map of transcription levels of chemokine and complement genes associated with synaptic pruning in the hippocampus of DRD2 ko mice. ( B ) IS rats exhibit elevated hippocampal mRNA expression of chemokines associated with synaptic pruning; n = 6 rats per group. ( C ) and ( D ) Immunofluorescence co-labelling of neurons (green) and CCL2 (red) in the dg. Quinpirole decreased CCL2 expression in neurons, while sulpiride increased it. Scale bar = 100 μm, n = 4 rats per group. ( E ) Representative immunofluorescence image showing co-localization of dg microglia (green) and CCR2 (red) (indicated by white arrows). Data are shown as mean ± sem, with unpaired Student's t-test, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: The Journal of Headache and Pain

Article Title: Downregulation of neuronal DRD2 drives microglia synaptic pruning and results in cognitive deficits by promoting CCL2 release in a rat model of chronic migraine

doi: 10.1186/s10194-025-02229-3

Figure Lengend Snippet: IS rats exhibit elevated CCL2 expression in hippocampal neurons, which DRD2 regulates. ( A ) Heat map of transcription levels of chemokine and complement genes associated with synaptic pruning in the hippocampus of DRD2 ko mice. ( B ) IS rats exhibit elevated hippocampal mRNA expression of chemokines associated with synaptic pruning; n = 6 rats per group. ( C ) and ( D ) Immunofluorescence co-labelling of neurons (green) and CCL2 (red) in the dg. Quinpirole decreased CCL2 expression in neurons, while sulpiride increased it. Scale bar = 100 μm, n = 4 rats per group. ( E ) Representative immunofluorescence image showing co-localization of dg microglia (green) and CCR2 (red) (indicated by white arrows). Data are shown as mean ± sem, with unpaired Student's t-test, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: DRD2 , Santa Cruz, USA , Sc-5303 , Mouse , 1:200.

Techniques: Expressing, Immunofluorescence

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