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anti cd86  (Boster Bio)


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    Structured Review

    Boster Bio anti cd86
    Anti Cd86, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd86/Anti-CD86+(Dendritic+Cells+Maturation+Marker)+Monoclonal+Antibody/pmc12860705-623-9-13
    Average 94 stars, based on 3 article reviews
    anti cd86 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Neuroprotective Effects of C-Phycocyanin in Chronic Unpredictable Mild Stress-Exposed Rats: Regulation of Neuroinflammation and CREB/BDNF Signaling.
    Article Snippet: 1 Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2 Behavioral Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran 3 Department of Radiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran 4 Electrophysiology Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran 5 Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran 6 Ross and Carol Nese College of Nursing, Pennsylvania State University, University ParkPA, USA 7 Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA 8 Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran 9 Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran Abstract Depression is a widespread neuropsychiatric disorder that significantly impacts emotional and cognitive function.. Antidepressant medications are frequently accompanied by various adverse effects.. C-phycocyanin has been previously shown to exert potent anti-inflammatory, and neuroprotective properties.

    Western Blot:

    Article Title: The activation of hippocampus microglia in the occurrence of airway hyperresponsiveness comorbidity anxiety.
    Article Snippet: After electrophoresis at 100 V for 90 min, the gels were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, US) for wet electric transfer at 250 mA for 70 min. After being blocked with 5% BSA for 1 h to block the nonspecific sites, the PVDF membranes were then incubated in primary antibodies overnight at 4 °C. .. Primary antibodies used for western blot included anti-NLRP3 (1 : 800, ET1610-93, HUABIO), anti-Caspase 1 (1 : 800, ET160869, HUABIO), anti-IL-1β(1 :800, #HA601002, HUABIO), anti-β-Tubulin (1 : 7000,10094-1-AP, Proteintech), anti-Iba1(1:2000, 17198 T, Cell Signaling Technology), anti-CD86(1:2000, BM4121, Boster), and anti-β-actin (1 : 7000, 20536-1-AP, Proteintech). .. Anti-mouse or antirabbit horseradish peroxidase-conjugated-IgG secondary antibody (Proteintech, China) was used to detect the binding of primary antibodies and imaged with BeyoECL Plus detection reagent (cat. no. P0018S; Beyotime Biotechnology Institute of Biotechnology) using the Chemi-Doc Imaging System (cat. no. 12003153; Bio-Rad Laboratories, Inc.).

    Article Title: The activation of hippocampus microglia in the occurrence of airway hyperresponsiveness comorbidity anxiety
    Article Snippet: After electrophoresis at 100 V for 90 min, the gels were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, US) for wet electric transfer at 250 mA for 70 min. After being blocked with 5% BSA for 1 h to block the nonspecific sites, the PVDF membranes were then incubated in primary antibodies overnight at 4 °C. .. Primary antibodies used for western blot included anti-NLRP3 (1 : 800, ET1610-93, HUABIO), anti-Caspase 1 (1 : 800, ET1608-69, HUABIO), anti-IL-1β(1 :800, # HA601002 , HUABIO), anti-β-Tubulin (1 : 7000,10094-1-AP, Proteintech), anti-Iba1(1:2000, 17198 T, Cell Signaling Technology), anti-CD86(1:2000, BM4121, Boster), and anti-β-actin (1 : 7000, 20536-1-AP, Proteintech). .. Anti-mouse or anti-rabbit horseradish peroxidase-conjugated-IgG secondary antibody (Proteintech, China) was used to detect the binding of primary antibodies and imaged with BeyoECL Plus detection reagent (cat. no. P0018S; Beyotime Biotechnology Institute of Biotechnology) using the Chemi-Doc Imaging System (cat. no. 12003153; Bio-Rad Laboratories, Inc.).

    Staining:

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway
    Article Snippet: .. Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis. ..

    Immunofluorescence:

    Article Title: Nanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway
    Article Snippet: .. Additionally, the sections were stained with antibodies specific to anti-CD86, CD206, and IL-1β (Boster Biological Technology Co., Ltd., Wuhan, China) for immunofluorescence analysis. ..



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    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and <t>CD86</t> surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
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    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and <t>CD86</t> surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.
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    In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker <t>CD86</t> and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.
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    In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker <t>CD86</t> and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.
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    In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker <t>CD86</t> and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.
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    Image Search Results


    Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Journal: Molecular Therapy Oncology

    Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

    doi: 10.1016/j.omton.2026.201185

    Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

    Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

    Techniques: Flow Cytometry

    In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Bioactive Materials

    Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

    doi: 10.1016/j.bioactmat.2025.12.055

    Figure Lengend Snippet: In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

    Techniques: In Vitro, Cell Culture, Staining, Marker, Fluorescence, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

    In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Bioactive Materials

    Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

    doi: 10.1016/j.bioactmat.2025.12.055

    Figure Lengend Snippet: In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

    Techniques: In Vivo, Immunohistochemistry, Staining