Review



cd44v6 antibody  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio-Rad cd44v6 antibody
    Cd44v6 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Rat+anti+Mouse+CD44v6/pm41455700-381-13-17
    Average 93 stars, based on 24 article reviews
    cd44v6 antibody - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Galectin-9 inhibition of the MIF-CD74/CD44 pathway suppresses chronic arthritis.
    Article Snippet: 1 Department of Biomedicine & Health Sciences, Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Korea; 2 Department of Rheumatology and Immunology, The Second Affiliated Hospital of Soochow University, Suzhou, China; 3 Center for Integrative Rheumatoid Transcriptomics and Dynamics, The Catholic University of Korea, Seoul, Korea; 4 Department of Internal Medicine, Inje University Ilsan Paik Hospital, Goyang, Korea; 5 GBIOLOGICS, Seongnam, Korea; 6 Department of Immunology of Yanbian University Hospital, Yanji, Jilin Province, China; 7 Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA; 8 Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea

    Immunohistochemical staining:

    Article Title: Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
    Article Snippet: Jejunums were dissected for fixation in 10% PFA (Electron Microscopy Sciences) in PBS for 24 hours at 4 degree, washed with 70% EtOH, embedded in paraffin, and 3-μm sections prepared and processed for haematoxylin staining and immunohistochemistry. .. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections using a Ventana BenchMark fully automated stains system with anti-ZO-1 (Thermo Fisher Scientific #33-9100, 1:50), anti-Beta-Catenin (BD transduction laboratories #610154, 1:200) or anti-CD44v6 (BioRad #MCA1967, 1:100). ..

    Staining:

    Article Title: Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
    Article Snippet: Jejunums were dissected for fixation in 10% PFA (Electron Microscopy Sciences) in PBS for 24 hours at 4 degree, washed with 70% EtOH, embedded in paraffin, and 3-μm sections prepared and processed for haematoxylin staining and immunohistochemistry. .. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections using a Ventana BenchMark fully automated stains system with anti-ZO-1 (Thermo Fisher Scientific #33-9100, 1:50), anti-Beta-Catenin (BD transduction laboratories #610154, 1:200) or anti-CD44v6 (BioRad #MCA1967, 1:100). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
    Article Snippet: Jejunums were dissected for fixation in 10% PFA (Electron Microscopy Sciences) in PBS for 24 hours at 4 degree, washed with 70% EtOH, embedded in paraffin, and 3-μm sections prepared and processed for haematoxylin staining and immunohistochemistry. .. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections using a Ventana BenchMark fully automated stains system with anti-ZO-1 (Thermo Fisher Scientific #33-9100, 1:50), anti-Beta-Catenin (BD transduction laboratories #610154, 1:200) or anti-CD44v6 (BioRad #MCA1967, 1:100). ..

    other:

    Article Title: Liver Cancer Initiation Requires p53 Inhibition by CD44-Enhanced Growth Factor Signaling
    Article Snippet: anti-CD44v6 , ABD Serotec (Biorad) , Cat# MCA1967; RRID: AB_323213.



    Similar Products

    anti cd44v6  (Miltenyi Biotec)
    94
    Miltenyi Biotec anti cd44v6
    Anti Cd44v6, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/CD44v6+Antibody%2C+anti-human%2C+REAfinity/pm41895662-108-7-10
    Average 94 stars, based on 1 article reviews
    anti cd44v6 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti lig3  (R&D Systems)
    93
    R&D Systems anti lig3
    Anti Lig3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Human+CD44v6+Antibody/pmc12987654-244-21-22
    Average 93 stars, based on 1 article reviews
    anti lig3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    lig3  (R&D Systems)
    93
    R&D Systems lig3
    Lig3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Human+CD44v6+Antibody/10__1172_slash_jci194949-259-24-25
    Average 93 stars, based on 1 article reviews
    lig3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    cd44v6 pe  (R&D Systems)
    93
    R&D Systems cd44v6 pe
    Cd44v6 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Human+CD44+v6+PE-conjugated+Antibody/pm41631682-53-25-27
    Average 93 stars, based on 1 article reviews
    cd44v6 pe - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    cd44v6 apc  (Miltenyi Biotec)
    94
    Miltenyi Biotec cd44v6 apc
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Cd44v6 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/CD44v6+Antibody%2C+anti-human%2C+REAfinity/bio_rxiv__64898__2026__01__07__697660-83-0-5
    Average 94 stars, based on 1 article reviews
    cd44v6 apc - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    cd44v6 antibody  (Bio-Rad)
    93
    Bio-Rad cd44v6 antibody
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Cd44v6 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Rat+anti+Mouse+CD44v6/pm41455700-381-13-17
    Average 93 stars, based on 1 article reviews
    cd44v6 antibody - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    rat anti cd44v6 antibody  (Bio-Rad)
    93
    Bio-Rad rat anti cd44v6 antibody
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Rat Anti Cd44v6 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Rat+anti+Mouse+CD44v6/pmc12864942-453-10-16
    Average 93 stars, based on 1 article reviews
    rat anti cd44v6 antibody - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    cd44v6 antibody anti human conjugated to apc  (Miltenyi Biotec)
    94
    Miltenyi Biotec cd44v6 antibody anti human conjugated to apc
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Cd44v6 Antibody Anti Human Conjugated To Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/CD44v6+Antibody%2C+anti-human%2C+APC%2C+REAfinity/pm40753649-320-25-31
    Average 94 stars, based on 1 article reviews
    cd44v6 antibody anti human conjugated to apc - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    anti cd44v6 antibody  (Boehringer Ingelheim)
    86
    Boehringer Ingelheim anti cd44v6 antibody
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Anti Cd44v6 Antibody, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/anti+antibody+cd44v6/sec_filing____855654_slash_000104746904027022_slash_a2139847z10___k-168-18-0
    Average 86 stars, based on 1 article reviews
    anti cd44v6 antibody - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    goat monoclonal anti cd44v6  (Bio-Rad)
    93
    Bio-Rad goat monoclonal anti cd44v6
    (a) Quantification of the <t>CD44v6-APC-positive</t> population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.
    Goat Monoclonal Anti Cd44v6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd44v6/Rat+anti+Mouse+CD44v6/pmc12319253-20-0-4
    Average 93 stars, based on 1 article reviews
    goat monoclonal anti cd44v6 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) Quantification of the CD44v6-APC-positive population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.

    Journal: bioRxiv

    Article Title: A Novel miR-4745 -KLC2 Axis Regulates Cancer Stem Cell Traits in Colorectal Cancer

    doi: 10.64898/2026.01.07.697660

    Figure Lengend Snippet: (a) Quantification of the CD44v6-APC-positive population in CPP1 (n=7) and CTC44 (n=5) cells transfected with miR-4745-3p ( miR4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics (right panel). Percentage of CD44v6-APC positive CPP1 cells is indicated in inset boxes for a representative experiment (left panel). (b) Representative images of tumorspheres (upper panel) and the percentage (lower panel) of sphere-forming cells in patient-derived CPP1 and circulating tumor (CTC44) colon cancer cells transfected with miR-4745-3p ( miR4745 ) or miCTRL mimics. Data from individual replicates (n=15 per experiment) are presented, along with the mean ± SEM from six independent experiments. (c) Percentage of sphere-forming cells in patient-derived CPP1 colon cancer cells transfected with miR4745 or negative control (miCTRL) mimics and, maintained as first-generation colonospheres (S1) or after several passages (S2, S3). Data from individual replicates (n=12 per experiment) are presented, along with the mean ± SEM from three independent experiments. (d) Percentage of surviving cancer stem cells (Aldefluor-positive) measured 48 hours following treatment with specified concentrations of FIRI (1X = 50 µM 5-FU + 500 nM SN38) in vitro . Sorted CPP1 Aldefluor-positive cells were first transfected with 50 nM miR-4745-3p ( miR-4745 ) or miRVana microRNA Mimic Negative Control #1 (miCTRL) mimics, 24 hours prior to FIRI treatment. Data are expressed as mean ± SEM (n = 3). (e) Percentage of Aldefluor-positive cells (% ALDH + cells) in CPP1 cells transfected with miR-4745 or miCTRL mimics and then exposed for 72 hours to chemotherapy (Firi=5µM 5-FU + 50nM SN38). Data are expressed as mean ± SEM (n=6). *, p<0.05; **, p<0.005, ***, p < 0.001, Mann Whitney test.

    Article Snippet: CD44v6-APC (clone REA706, 130-111-238; Macs Miltenyi) antibody was incubated with 100 000 cells in PBS containing 5% FBS for 20 min at 4°C.

    Techniques: Transfection, Negative Control, Derivative Assay, In Vitro, MANN-WHITNEY