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cd38  (R&D Systems)


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    R&D Systems cd38
    Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates <t>CD38</t> cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Cd38, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd38/Mouse+CD38+Antibody/pmc13059309-44-8-27
    Average 93 stars, based on 25 article reviews
    cd38 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Injectable antifibrotic drug-loaded hydrogels reduce fibrosis and restore myogenesis by enhancing mitochondrial metabolism and cell mechanics in an in vitro coculture model"

    Article Title: Injectable antifibrotic drug-loaded hydrogels reduce fibrosis and restore myogenesis by enhancing mitochondrial metabolism and cell mechanics in an in vitro coculture model

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.103033

    Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates CD38 cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates CD38 cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Fluorescence, Marker, Expressing, Activation Assay, Immunostaining

    Related Articles

    Incubation:

    Article Title: Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination.
    Article Snippet: .. The membranes were then blocked in 5% skimmed milk for 30 min, incubated with anti-CD38 (AF4947, R&D systems, MN, United States, 1:500), anti-glial fibrillary acidic protein (GFAP) (G9269, Sigma, MO, United States, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500), or anti-myelin basic protein (MBP) (MAB396, Merck Millipore, Darmstadt, Germany, 1:1,000) antibodies for 12 h at 4◦C. ..

    Article Title: Inhibition of CD38 and supplementation of nicotinamide riboside ameliorate lipopolysaccharide-induced neuroinflammation in the hippocampus
    Article Snippet: .. Membranes were blocked in 5% skimmed milk for 30 min and incubated with anti-CD38 (AF4947, R&D systems, MN, USA, 1:500), anti-glial brillary acidic protein (GFAP) (G9269, Sigma, MO, USA, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500) antibodies for 16 h at 4 °C. .. Membranes were washed several times with Tris-buffered saline, 0.1% Tween 20 (TBST) and incubated with anti-rabbit (SC-2004, 1:5,000, Santa Cruz Biotechnology), anti-mouse (SC-516102, 1:5,000, Santa Cruz Biotechnology), anti-goat (SC-2354, 1:1,000, Santa Cruz Biotechnology), or anti-rat (NA9350, Amersham Pharmacia biotech, 1:1000), and horseradish peroxidase-linked immunoglobulin G (Cell Signaling Technology, Tokyo, Japan) for 2 h at room temperature, and the membranes were washed several times with TBST.

    Article Title: Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination
    Article Snippet: .. The membranes were then blocked in 5% skimmed milk for 30 min, incubated with anti-CD38 (AF4947, R&D systems, MN, United States, 1:500), anti-glial fibrillary acidic protein (GFAP) (G9269, Sigma, MO, United States, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500), or anti-myelin basic protein (MBP) (MAB396, Merck Millipore, Darmstadt, Germany, 1:1,000) antibodies for 12 h at 4°C. ..

    Article Title: CXCR5 + CD4 + T Follicular Helper Cells Participate in the Pathogenesis of Primary Biliary Cirrhosis
    Article Snippet: .. Sections were incubated with optimal concentrations of primary monoclonal antibodies, including anti-CD38, anti-CD20, anti-CD4, anti-PD-1 (R&D, AF1086, Abington, UK) and anti-Bcl-6 (Santa Cruz biotechnology, sc-858, Santa Cruz, CA, USA) for 1 hr at RT and then incubated with biotinylated goat anti-rat, goat-rabbit and biotinylated rabbit anti-mouse antibody (Zhongshan Goldenbridge Biotech, Beijing, China), respectively. .. The avidin-biotin-peroxidase system with 3-amino-9-ethyl-carbazole (AEC) (red color) or Vector blue (blue color) as substrates was used to perform double staining.

    Article Title: Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory.
    Article Snippet: Social behavior is essential for health, survival, and reproduction of animals; however, the role of astrocytes in social behavior remains largely unknown.. The transmembrane protein CD38, which acts both as a receptor and ADP-ribosyl cyclase to produce cyclic ADP–ribose (cADPR) regulates social behaviors by promoting oxytocin release from hypothalamic neurons.. CD38 is also abundantly expressed in astrocytes in the postnatal brain and is important for astroglial development.

    Binding Assay:

    Article Title: Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination.
    Article Snippet: .. The membranes were then blocked in 5% skimmed milk for 30 min, incubated with anti-CD38 (AF4947, R&D systems, MN, United States, 1:500), anti-glial fibrillary acidic protein (GFAP) (G9269, Sigma, MO, United States, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500), or anti-myelin basic protein (MBP) (MAB396, Merck Millipore, Darmstadt, Germany, 1:1,000) antibodies for 12 h at 4◦C. ..

    Article Title: Inhibition of CD38 and supplementation of nicotinamide riboside ameliorate lipopolysaccharide-induced neuroinflammation in the hippocampus
    Article Snippet: .. Membranes were blocked in 5% skimmed milk for 30 min and incubated with anti-CD38 (AF4947, R&D systems, MN, USA, 1:500), anti-glial brillary acidic protein (GFAP) (G9269, Sigma, MO, USA, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500) antibodies for 16 h at 4 °C. .. Membranes were washed several times with Tris-buffered saline, 0.1% Tween 20 (TBST) and incubated with anti-rabbit (SC-2004, 1:5,000, Santa Cruz Biotechnology), anti-mouse (SC-516102, 1:5,000, Santa Cruz Biotechnology), anti-goat (SC-2354, 1:1,000, Santa Cruz Biotechnology), or anti-rat (NA9350, Amersham Pharmacia biotech, 1:1000), and horseradish peroxidase-linked immunoglobulin G (Cell Signaling Technology, Tokyo, Japan) for 2 h at room temperature, and the membranes were washed several times with TBST.

    Article Title: Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination
    Article Snippet: .. The membranes were then blocked in 5% skimmed milk for 30 min, incubated with anti-CD38 (AF4947, R&D systems, MN, United States, 1:500), anti-glial fibrillary acidic protein (GFAP) (G9269, Sigma, MO, United States, 1:5,000), anti-ionized calcium binding adaptor molecule 1 (Iba1) (019-19741, Wako, Osaka, Japan, 1:500), or anti-myelin basic protein (MBP) (MAB396, Merck Millipore, Darmstadt, Germany, 1:1,000) antibodies for 12 h at 4°C. ..

    Membrane:

    Article Title: CRTAM+NK cells endowed with suppressor properties arise in leukemic bone marrow
    Article Snippet: CD34+ cells from MPB or UCB were enriched formost experiments using theHumanCD34Progenitor Cell Isolation Kit (Miltenyi Biotec, Germany) according to manufacturer’s instructions. .. After membrane staining of mononuclear or enriched cells with PE-conjugated anti-lineage markers (BD Biosciences, USA or Biolegend, USA) (CD3, CD8, TCR, CD56, CD14, CD11b, CD20, CD19, and CD235a), anti-CD34–PB, anti-CD38–FITC, anti-CD45RA– PE-Cy5, anti-CD16–FITC, anti-CD56–PE, anti-CRTAM–APC (R&D Systems, USA), or anti-TGF-β–PE (Biolegend, USA) conjugated antibodies, samples were acquired in the FACS Canto II cytometer and the data analyzed with the FlowJo 10 software (TreeStar Inc, BD Biosciences, USA). .. Hematopoietic stem and progenitor cell (HSPC) fractions, including Lin−CD34+CD38−CD45RA− hematopoietic stem cells (HSCs), Lin−CD34+CD38−CD45RA+ multilymphoid progenitors (MLPs), Lin−CD34+CD38+CD45RA− multipotent progenitors (MPPs), and Lin−CD34+CD38−CD45RA+ LPs, were highly purified by multiparametric flow cytometry sorting in a BD FACS Aria sorter.

    Article Title: Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory.
    Article Snippet: Social behavior is essential for health, survival, and reproduction of animals; however, the role of astrocytes in social behavior remains largely unknown.. The transmembrane protein CD38, which acts both as a receptor and ADP-ribosyl cyclase to produce cyclic ADP–ribose (cADPR) regulates social behaviors by promoting oxytocin release from hypothalamic neurons.. CD38 is also abundantly expressed in astrocytes in the postnatal brain and is important for astroglial development.

    Staining:

    Article Title: CRTAM+NK cells endowed with suppressor properties arise in leukemic bone marrow
    Article Snippet: CD34+ cells from MPB or UCB were enriched formost experiments using theHumanCD34Progenitor Cell Isolation Kit (Miltenyi Biotec, Germany) according to manufacturer’s instructions. .. After membrane staining of mononuclear or enriched cells with PE-conjugated anti-lineage markers (BD Biosciences, USA or Biolegend, USA) (CD3, CD8, TCR, CD56, CD14, CD11b, CD20, CD19, and CD235a), anti-CD34–PB, anti-CD38–FITC, anti-CD45RA– PE-Cy5, anti-CD16–FITC, anti-CD56–PE, anti-CRTAM–APC (R&D Systems, USA), or anti-TGF-β–PE (Biolegend, USA) conjugated antibodies, samples were acquired in the FACS Canto II cytometer and the data analyzed with the FlowJo 10 software (TreeStar Inc, BD Biosciences, USA). .. Hematopoietic stem and progenitor cell (HSPC) fractions, including Lin−CD34+CD38−CD45RA− hematopoietic stem cells (HSCs), Lin−CD34+CD38−CD45RA+ multilymphoid progenitors (MLPs), Lin−CD34+CD38+CD45RA− multipotent progenitors (MPPs), and Lin−CD34+CD38−CD45RA+ LPs, were highly purified by multiparametric flow cytometry sorting in a BD FACS Aria sorter.

    FACS:

    Article Title: CRTAM+NK cells endowed with suppressor properties arise in leukemic bone marrow
    Article Snippet: CD34+ cells from MPB or UCB were enriched formost experiments using theHumanCD34Progenitor Cell Isolation Kit (Miltenyi Biotec, Germany) according to manufacturer’s instructions. .. After membrane staining of mononuclear or enriched cells with PE-conjugated anti-lineage markers (BD Biosciences, USA or Biolegend, USA) (CD3, CD8, TCR, CD56, CD14, CD11b, CD20, CD19, and CD235a), anti-CD34–PB, anti-CD38–FITC, anti-CD45RA– PE-Cy5, anti-CD16–FITC, anti-CD56–PE, anti-CRTAM–APC (R&D Systems, USA), or anti-TGF-β–PE (Biolegend, USA) conjugated antibodies, samples were acquired in the FACS Canto II cytometer and the data analyzed with the FlowJo 10 software (TreeStar Inc, BD Biosciences, USA). .. Hematopoietic stem and progenitor cell (HSPC) fractions, including Lin−CD34+CD38−CD45RA− hematopoietic stem cells (HSCs), Lin−CD34+CD38−CD45RA+ multilymphoid progenitors (MLPs), Lin−CD34+CD38+CD45RA− multipotent progenitors (MPPs), and Lin−CD34+CD38−CD45RA+ LPs, were highly purified by multiparametric flow cytometry sorting in a BD FACS Aria sorter.

    Cytometry:

    Article Title: CRTAM+NK cells endowed with suppressor properties arise in leukemic bone marrow
    Article Snippet: CD34+ cells from MPB or UCB were enriched formost experiments using theHumanCD34Progenitor Cell Isolation Kit (Miltenyi Biotec, Germany) according to manufacturer’s instructions. .. After membrane staining of mononuclear or enriched cells with PE-conjugated anti-lineage markers (BD Biosciences, USA or Biolegend, USA) (CD3, CD8, TCR, CD56, CD14, CD11b, CD20, CD19, and CD235a), anti-CD34–PB, anti-CD38–FITC, anti-CD45RA– PE-Cy5, anti-CD16–FITC, anti-CD56–PE, anti-CRTAM–APC (R&D Systems, USA), or anti-TGF-β–PE (Biolegend, USA) conjugated antibodies, samples were acquired in the FACS Canto II cytometer and the data analyzed with the FlowJo 10 software (TreeStar Inc, BD Biosciences, USA). .. Hematopoietic stem and progenitor cell (HSPC) fractions, including Lin−CD34+CD38−CD45RA− hematopoietic stem cells (HSCs), Lin−CD34+CD38−CD45RA+ multilymphoid progenitors (MLPs), Lin−CD34+CD38+CD45RA− multipotent progenitors (MPPs), and Lin−CD34+CD38−CD45RA+ LPs, were highly purified by multiparametric flow cytometry sorting in a BD FACS Aria sorter.

    Software:

    Article Title: CRTAM+NK cells endowed with suppressor properties arise in leukemic bone marrow
    Article Snippet: CD34+ cells from MPB or UCB were enriched formost experiments using theHumanCD34Progenitor Cell Isolation Kit (Miltenyi Biotec, Germany) according to manufacturer’s instructions. .. After membrane staining of mononuclear or enriched cells with PE-conjugated anti-lineage markers (BD Biosciences, USA or Biolegend, USA) (CD3, CD8, TCR, CD56, CD14, CD11b, CD20, CD19, and CD235a), anti-CD34–PB, anti-CD38–FITC, anti-CD45RA– PE-Cy5, anti-CD16–FITC, anti-CD56–PE, anti-CRTAM–APC (R&D Systems, USA), or anti-TGF-β–PE (Biolegend, USA) conjugated antibodies, samples were acquired in the FACS Canto II cytometer and the data analyzed with the FlowJo 10 software (TreeStar Inc, BD Biosciences, USA). .. Hematopoietic stem and progenitor cell (HSPC) fractions, including Lin−CD34+CD38−CD45RA− hematopoietic stem cells (HSCs), Lin−CD34+CD38−CD45RA+ multilymphoid progenitors (MLPs), Lin−CD34+CD38+CD45RA− multipotent progenitors (MPPs), and Lin−CD34+CD38−CD45RA+ LPs, were highly purified by multiparametric flow cytometry sorting in a BD FACS Aria sorter.

    Bioprocessing:

    Article Title: CXCR5 + CD4 + T Follicular Helper Cells Participate in the Pathogenesis of Primary Biliary Cirrhosis
    Article Snippet: .. Sections were incubated with optimal concentrations of primary monoclonal antibodies, including anti-CD38, anti-CD20, anti-CD4, anti-PD-1 (R&D, AF1086, Abington, UK) and anti-Bcl-6 (Santa Cruz biotechnology, sc-858, Santa Cruz, CA, USA) for 1 hr at RT and then incubated with biotinylated goat anti-rat, goat-rabbit and biotinylated rabbit anti-mouse antibody (Zhongshan Goldenbridge Biotech, Beijing, China), respectively. .. The avidin-biotin-peroxidase system with 3-amino-9-ethyl-carbazole (AEC) (red color) or Vector blue (blue color) as substrates was used to perform double staining.

    Blocking Assay:

    Article Title: Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory.
    Article Snippet: Social behavior is essential for health, survival, and reproduction of animals; however, the role of astrocytes in social behavior remains largely unknown.. The transmembrane protein CD38, which acts both as a receptor and ADP-ribosyl cyclase to produce cyclic ADP–ribose (cADPR) regulates social behaviors by promoting oxytocin release from hypothalamic neurons.. CD38 is also abundantly expressed in astrocytes in the postnatal brain and is important for astroglial development.

    other:

    Article Title: Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex
    Article Snippet: The antibodies used in this study are as follows: anti-CD38 (AF4947, R&D systems, Minneapolis, MN, USA, 1:300); anti-β-actin (013-24553, WAKO, Tokyo, Japan, 1:2000); anti-GFAP (G9269, SIGMA, 1:2000); anti-VGlut1(AB5905, Millipore, Burlington, MA, USA, 1:2000); anti-PSD95 (51-6900, Thermo Fischer Scientific, Waltham, MA, USA, 1:500); anti-NDRG2 (sc-19468, Santa Cruz Biotechnology, In, Dallas, TX, USA, 1:500); anti-MAP2 (4542S, Cell signaling, Danvers, MA, USA, 1:200); anti-Caspase-3 (9661S, Cell signaling, 1:300); anti-Cx43 (MAB3068, Merck Millipore, MA, USA, 1:200); anti-SPARCL1 (AF2836, R&D systems, 1:2000); anti-BDNF (ab108319, Abcam, Cambridge, MA, USA, 1:2000); anti-guinea pig (Abcam, ab150185, 1:300), anti-goat (Abcam, ab7121, 1:300) and anti-rabbit Alexa488-(A21206, Invitrogen, Waltham, MA, USA,1:300) and anti-rabbit and anti-mouse Cy3-conjugated secondary antibody (711-165-152 and 715-165-150, Jackson ImmunoResearch Laboratories, West Grove, PA, USA, 1:300); anti-rabbit (sc-2357, Santa Cruz Biotechnology, In, 1:5000), anti-mouse (sc-516102, Santa Cruz Biotechnology, In, 1:5000), anti-goat (sc-2354, Santa Cruz Biotechnology, In, 1:2000) and anti-guinea pig (sc-2438, Santa Cruz Biotechnology, In, 1:2000) horseradish peroxidase-linked immunoglobulin G. DAPI was purchased from Invitrogen (D1306, Thermo Fischer Scientific).



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    Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates <t>CD38</t> cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    anti human cd38 antibody anti hcd38  (Sanofi)
    86
    Sanofi anti human cd38 antibody anti hcd38
    Immune pathology, B-cell characterization, and tertiary lymphoid organ-related features. a Diffuse relatively mild sarcolemmal MHC cl. 1 positivity and presence of many MHC cl. 1 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 1 immunohistochemistry; original magnification ×200. b Mild and infrequent sarcolemmal MHC cl. 2 positivity and presence of many MHC cl. 2 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 2 immunohistochemistry; original magnification ×200. c Sarcolemmal complement deposits (arrow) and presence of single necrotic myofibers (arrowhead) that show sarcoplasmic complement deposits. C5b-9 (MAC) immunohistochemistry; original magnification ×200. d Presence of numerous endomysial macrophages and occasional myophagocytoses. CD68 immunohistochemistry; original magnification ×200. e Scattered, individual endomysial T cells. CD8 immunohistochemistry; original magnification ×200. f Focally clustering B cells with a roundish appearance reminiscent of so-called tertiary lymphoid organs (TLOs). CD20 immunohistochemistry; original magnification ×200. g , h TLO-like immune cell clusters show compartmentalization with bcl2-positive B-cell nuclei at the margins and bcl6-positive nuclei in their (germinal) center. Bcl2 (G) and bcl6 (H) immunohistochemistry; original magnification ×600. i Numerous Ki67 + proliferating cells are focally clustering predominantly in germinal centers of TLOs. Ki67 immunohistochemistry; original magnification ×600. j A B-cell cluster contains some CD138-positive plasma cells. CD138 immunohistochemistry; original magnification ×200. k Similarly, those plasma cells and a few B cells are identified by nuclear MUM1 positivity in the light zone of a germinal center. MUM1 immunohistochemistry; original magnification ×200. l <t>CD38-positive</t> plasmablasts are partly proliferating and show co-immunoreactivity with Ki67. CD38 AF488 (green) and Ki67 Cy3 (red) immunofluorescence; original magnification ×200. a – l Scale bar represents 100 µm
    Anti Human Cd38 Antibody Anti Hcd38, supplied by Sanofi, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd38/sar650984/pm41906599-140-3-20
    Average 86 stars, based on 1 article reviews
    anti human cd38 antibody anti hcd38 - by Bioz Stars, 2026-09
    86/100 stars
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    pe vio770 miltenyi biotec 130 125 522 rat anti mouse cd4  (Miltenyi Biotec)
    92
    Miltenyi Biotec pe vio770 miltenyi biotec 130 125 522 rat anti mouse cd4
    Immune pathology, B-cell characterization, and tertiary lymphoid organ-related features. a Diffuse relatively mild sarcolemmal MHC cl. 1 positivity and presence of many MHC cl. 1 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 1 immunohistochemistry; original magnification ×200. b Mild and infrequent sarcolemmal MHC cl. 2 positivity and presence of many MHC cl. 2 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 2 immunohistochemistry; original magnification ×200. c Sarcolemmal complement deposits (arrow) and presence of single necrotic myofibers (arrowhead) that show sarcoplasmic complement deposits. C5b-9 (MAC) immunohistochemistry; original magnification ×200. d Presence of numerous endomysial macrophages and occasional myophagocytoses. CD68 immunohistochemistry; original magnification ×200. e Scattered, individual endomysial T cells. CD8 immunohistochemistry; original magnification ×200. f Focally clustering B cells with a roundish appearance reminiscent of so-called tertiary lymphoid organs (TLOs). CD20 immunohistochemistry; original magnification ×200. g , h TLO-like immune cell clusters show compartmentalization with bcl2-positive B-cell nuclei at the margins and bcl6-positive nuclei in their (germinal) center. Bcl2 (G) and bcl6 (H) immunohistochemistry; original magnification ×600. i Numerous Ki67 + proliferating cells are focally clustering predominantly in germinal centers of TLOs. Ki67 immunohistochemistry; original magnification ×600. j A B-cell cluster contains some CD138-positive plasma cells. CD138 immunohistochemistry; original magnification ×200. k Similarly, those plasma cells and a few B cells are identified by nuclear MUM1 positivity in the light zone of a germinal center. MUM1 immunohistochemistry; original magnification ×200. l <t>CD38-positive</t> plasmablasts are partly proliferating and show co-immunoreactivity with Ki67. CD38 AF488 (green) and Ki67 Cy3 (red) immunofluorescence; original magnification ×200. a – l Scale bar represents 100 µm
    Pe Vio770 Miltenyi Biotec 130 125 522 Rat Anti Mouse Cd4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd38/CD38+Antibody%2C+anti-mouse%2C+REAfinity/pm41916319-875-86-88
    Average 92 stars, based on 1 article reviews
    pe vio770 miltenyi biotec 130 125 522 rat anti mouse cd4 - by Bioz Stars, 2026-09
    92/100 stars
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    anti cd38  (Cell Signaling Technology Inc)
    94
    Cell Signaling Technology Inc anti cd38
    Immune pathology, B-cell characterization, and tertiary lymphoid organ-related features. a Diffuse relatively mild sarcolemmal MHC cl. 1 positivity and presence of many MHC cl. 1 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 1 immunohistochemistry; original magnification ×200. b Mild and infrequent sarcolemmal MHC cl. 2 positivity and presence of many MHC cl. 2 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 2 immunohistochemistry; original magnification ×200. c Sarcolemmal complement deposits (arrow) and presence of single necrotic myofibers (arrowhead) that show sarcoplasmic complement deposits. C5b-9 (MAC) immunohistochemistry; original magnification ×200. d Presence of numerous endomysial macrophages and occasional myophagocytoses. CD68 immunohistochemistry; original magnification ×200. e Scattered, individual endomysial T cells. CD8 immunohistochemistry; original magnification ×200. f Focally clustering B cells with a roundish appearance reminiscent of so-called tertiary lymphoid organs (TLOs). CD20 immunohistochemistry; original magnification ×200. g , h TLO-like immune cell clusters show compartmentalization with bcl2-positive B-cell nuclei at the margins and bcl6-positive nuclei in their (germinal) center. Bcl2 (G) and bcl6 (H) immunohistochemistry; original magnification ×600. i Numerous Ki67 + proliferating cells are focally clustering predominantly in germinal centers of TLOs. Ki67 immunohistochemistry; original magnification ×600. j A B-cell cluster contains some CD138-positive plasma cells. CD138 immunohistochemistry; original magnification ×200. k Similarly, those plasma cells and a few B cells are identified by nuclear MUM1 positivity in the light zone of a germinal center. MUM1 immunohistochemistry; original magnification ×200. l <t>CD38-positive</t> plasmablasts are partly proliferating and show co-immunoreactivity with Ki67. CD38 AF488 (green) and Ki67 Cy3 (red) immunofluorescence; original magnification ×200. a – l Scale bar represents 100 µm
    Anti Cd38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd38/CD38+Antibody/pm41899467-76-38-43
    Average 94 stars, based on 1 article reviews
    anti cd38 - by Bioz Stars, 2026-09
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    Image Search Results


    Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates CD38 cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Injectable antifibrotic drug-loaded hydrogels reduce fibrosis and restore myogenesis by enhancing mitochondrial metabolism and cell mechanics in an in vitro coculture model

    doi: 10.1016/j.mtbio.2026.103033

    Figure Lengend Snippet: Establishment and characterization of a fibrotic macrophage-muscle fibrosis model. a) Brightfield image of unstimulated RAW264.7 cells in the top chamber of a RAW-C2C12 coculture system, b) Brightfield image of C2C12 cells in the bottom chamber under basal coculture conditions, c) Fluorescence image of C2C12 cells in the bottom chamber exhibiting myotube morphology, d) Fluorescence image confirming the absence of fibrotic marker expression in C2C12 cells under basal conditions, e) Brightfield image of LPS-activated RAW264.7 cells in the top chamber, f) Brightfield image of C2C12 cells in the bottom chamber displaying a myofibroblast-like morphology post-LPS activation, g) Fluorescence image of phenotypic transition of C2C12 cells to myofibroblast-like cells, h) Fluorescence image demonstrating fibrotic protein expression in LPS-treated C2C12 cells (magenta arrow indicates CD38 cells). Immunostaining images: In panels c) and g), TGF-β, DAPI, and actin are colored red, blue, and green, respectively. In panels d) and h), COL1, α-SMA, CD38, and DAPI are colored red, blue, magenta, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Mouse monoclonal antibodies against myosin heavy chain (MyHC), CD38, and transforming growth factor-β (TGF-β), as well as rabbit polyclonal α-smooth muscle actin (α-SMA) antibodies, were purchased from R&D Systems (USA).

    Techniques: Fluorescence, Marker, Expressing, Activation Assay, Immunostaining

    Reconstitution of B-cells over time. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric). The subsets of gated CD19 + cells were identified based on surface marker expression as follows: naïve (CD19+CD27-IgD+), nonswitched memory (CD19+CD27+IgD+), switched memory (CD19+CD27+IgD-), and double negative (CD19+CD27-IgD-); and within the CD19+CD38++ population, we distinguished transitional cells (CD19+CD38++CD27-IgD+), plasmablasts (CD19+CD38++CD27+IgD-), and double-negative CD38 + cells (CD19+CD38++CD27-IgD-). B-cell subsets were expressed as a percentage of the total lymphocyte count. (a) B-cell subpopulations were assessed in 39 patients at baseline and in 36 patients at 3 (M3), 6 (M6), 9 (M9), 12 (M12), and 18 (M18) months following the first rituximab infusion. The 3 patients who did not receive treatment were excluded from the follow-up. Complete depletion of B-cells was observed in all patients at M3 post-rituximab for all B-cell subpopulations. CD19 + cells reappeared 6 months after rituximab infusion. Naive cells re-emerged the most among B-cells, followed by CD38 + cells, transitional cells and finally memory cells. Data are shown as mean values (dots). (b–k) B-cell subpopulations at baseline and at subsequent time points were compared between relapsing patients ( n = 8) and nonrelapsing patients ( n = 19); the 2 patients who received additional anti-CD20 infusions were excluded from subsequent analyses. Data are shown as medians and interquartile range (IQR). P -values were calculated by comparing the median values of each cell subpopulation between relapsing and nonrelapsing patients using a nonparametric, unpaired Mann–Whitney U test.

    Journal: Kidney International Reports

    Article Title: Early-Stage B-cells Predict Relapse After Rituximab Treatment in Patients With Membranous Nephropathy

    doi: 10.1016/j.ekir.2026.106365

    Figure Lengend Snippet: Reconstitution of B-cells over time. To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech). The stained cells were then analyzed using multicolor flow cytometry (BD FACS Lyric). The subsets of gated CD19 + cells were identified based on surface marker expression as follows: naïve (CD19+CD27-IgD+), nonswitched memory (CD19+CD27+IgD+), switched memory (CD19+CD27+IgD-), and double negative (CD19+CD27-IgD-); and within the CD19+CD38++ population, we distinguished transitional cells (CD19+CD38++CD27-IgD+), plasmablasts (CD19+CD38++CD27+IgD-), and double-negative CD38 + cells (CD19+CD38++CD27-IgD-). B-cell subsets were expressed as a percentage of the total lymphocyte count. (a) B-cell subpopulations were assessed in 39 patients at baseline and in 36 patients at 3 (M3), 6 (M6), 9 (M9), 12 (M12), and 18 (M18) months following the first rituximab infusion. The 3 patients who did not receive treatment were excluded from the follow-up. Complete depletion of B-cells was observed in all patients at M3 post-rituximab for all B-cell subpopulations. CD19 + cells reappeared 6 months after rituximab infusion. Naive cells re-emerged the most among B-cells, followed by CD38 + cells, transitional cells and finally memory cells. Data are shown as mean values (dots). (b–k) B-cell subpopulations at baseline and at subsequent time points were compared between relapsing patients ( n = 8) and nonrelapsing patients ( n = 19); the 2 patients who received additional anti-CD20 infusions were excluded from subsequent analyses. Data are shown as medians and interquartile range (IQR). P -values were calculated by comparing the median values of each cell subpopulation between relapsing and nonrelapsing patients using a nonparametric, unpaired Mann–Whitney U test.

    Article Snippet: To characterize B-cell subsets, peripheral blood mononuclear cells were stained with fluorochrome-conjugated monoclonal antibodies (BD Biosciences) directed against the following antigens: CD45 (V500-C), CD3 (APC), CD19 (APC-H7), CD27 (BV421), IgD (PE), CD38 (BV711), CD4 (BV605), and CD8 (PE), as well as 7-AAD (Miltenyi Biotech).

    Techniques: Staining, Bioprocessing, Flow Cytometry, Marker, Expressing, MANN-WHITNEY

    Immune pathology, B-cell characterization, and tertiary lymphoid organ-related features. a Diffuse relatively mild sarcolemmal MHC cl. 1 positivity and presence of many MHC cl. 1 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 1 immunohistochemistry; original magnification ×200. b Mild and infrequent sarcolemmal MHC cl. 2 positivity and presence of many MHC cl. 2 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 2 immunohistochemistry; original magnification ×200. c Sarcolemmal complement deposits (arrow) and presence of single necrotic myofibers (arrowhead) that show sarcoplasmic complement deposits. C5b-9 (MAC) immunohistochemistry; original magnification ×200. d Presence of numerous endomysial macrophages and occasional myophagocytoses. CD68 immunohistochemistry; original magnification ×200. e Scattered, individual endomysial T cells. CD8 immunohistochemistry; original magnification ×200. f Focally clustering B cells with a roundish appearance reminiscent of so-called tertiary lymphoid organs (TLOs). CD20 immunohistochemistry; original magnification ×200. g , h TLO-like immune cell clusters show compartmentalization with bcl2-positive B-cell nuclei at the margins and bcl6-positive nuclei in their (germinal) center. Bcl2 (G) and bcl6 (H) immunohistochemistry; original magnification ×600. i Numerous Ki67 + proliferating cells are focally clustering predominantly in germinal centers of TLOs. Ki67 immunohistochemistry; original magnification ×600. j A B-cell cluster contains some CD138-positive plasma cells. CD138 immunohistochemistry; original magnification ×200. k Similarly, those plasma cells and a few B cells are identified by nuclear MUM1 positivity in the light zone of a germinal center. MUM1 immunohistochemistry; original magnification ×200. l CD38-positive plasmablasts are partly proliferating and show co-immunoreactivity with Ki67. CD38 AF488 (green) and Ki67 Cy3 (red) immunofluorescence; original magnification ×200. a – l Scale bar represents 100 µm

    Journal: Acta Neuropathologica

    Article Title: Brachio-cervical inflammatory myopathy: multilevel clinical, histopathological and multi-omic analyses of a syndrome variably associated with systemic sclerosis

    doi: 10.1007/s00401-026-03006-5

    Figure Lengend Snippet: Immune pathology, B-cell characterization, and tertiary lymphoid organ-related features. a Diffuse relatively mild sarcolemmal MHC cl. 1 positivity and presence of many MHC cl. 1 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 1 immunohistochemistry; original magnification ×200. b Mild and infrequent sarcolemmal MHC cl. 2 positivity and presence of many MHC cl. 2 positive lymphomonocytic cells in the endomysium, and in a B-cell cluster (arrow). MHC cl. 2 immunohistochemistry; original magnification ×200. c Sarcolemmal complement deposits (arrow) and presence of single necrotic myofibers (arrowhead) that show sarcoplasmic complement deposits. C5b-9 (MAC) immunohistochemistry; original magnification ×200. d Presence of numerous endomysial macrophages and occasional myophagocytoses. CD68 immunohistochemistry; original magnification ×200. e Scattered, individual endomysial T cells. CD8 immunohistochemistry; original magnification ×200. f Focally clustering B cells with a roundish appearance reminiscent of so-called tertiary lymphoid organs (TLOs). CD20 immunohistochemistry; original magnification ×200. g , h TLO-like immune cell clusters show compartmentalization with bcl2-positive B-cell nuclei at the margins and bcl6-positive nuclei in their (germinal) center. Bcl2 (G) and bcl6 (H) immunohistochemistry; original magnification ×600. i Numerous Ki67 + proliferating cells are focally clustering predominantly in germinal centers of TLOs. Ki67 immunohistochemistry; original magnification ×600. j A B-cell cluster contains some CD138-positive plasma cells. CD138 immunohistochemistry; original magnification ×200. k Similarly, those plasma cells and a few B cells are identified by nuclear MUM1 positivity in the light zone of a germinal center. MUM1 immunohistochemistry; original magnification ×200. l CD38-positive plasmablasts are partly proliferating and show co-immunoreactivity with Ki67. CD38 AF488 (green) and Ki67 Cy3 (red) immunofluorescence; original magnification ×200. a – l Scale bar represents 100 µm

    Article Snippet: The antibodies used for immunohistochemistry included C5b-9 (Dako, aE11, 1:200), CD8 (Dako, C8/144B, 1:100), CD20 (Dako, Ks 20.8 1:200), Ki-67 (Dako, Mib-1, 1:100), MxA (Millipore, MABF938, 1:100), CD38 (Novocastra SPC32, 1:100), MUM1 (Dako, MUM1P, 1:50), CD45 (Dako, 2B11, 1:400), CD56 (Serotec/MCA591 ERIC-1, 1:400), CD68 (Dako, EBM11, 1:100), MHC class I (Dako, w6/32, 1:1000), MHC class II (Dako, CR3/43, 1:100), and SQSTM1/p62 (Abcam, polyclonal, 1:100).

    Techniques: Cell Characterization, Immunohistochemistry, Clinical Proteomics, Immunofluorescence