anti cd31 antibody  (R&D Systems)

Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: mGFD‐CD3 induces hepatotoxicity by targeting hepatic endothelial cells in mice. (A) H&E analysis performed at 24 h post‐injection of BiTE in the liver tissues of naturally aged mice. Scale bar, 30 μm (original) and 15 μm (magnified). (B) Immunofluorescence analysis was conducted on liver, lung, and kidney samples from naturally aged mice collected 24 h post‐BiTE injection ( n = 6 view field per each treatment group). Scale bar, 30 μm. (C) Immunofluorescence analysis of liver samples of naturally aged mice taken 4 h post‐injection of BiTE to examine the colocalization of BiTE, CD31, and uPAR. Scale bar, 30 μm (original) and 15 μm (magnified). (D) Immunofluorescence analysis to examine the colocalization of CD31 and uPAR in the liver tissues of wild type mice (WT) and uPAR knockout mice; ( n = 3 mice per treatment group). Scale bar, 30 μm. (E) Levels of AST and ALT in serum samples from mice before and after BiTE injection were determined with a Biochemical Analyzer; ( n = 3 mice per treatment group). Data is presented as mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test with Welch's correction. *** p ≤ 0.001.

Article Snippet: All primary antibodies used in this work were commercially available: uPAR (Proteintech, 10286‐1‐AP, 1:200), CD31 (Proteintech, 28083‐1‐AP, 1:400), F4/80 (CST, 70076S, 1:200), and Fc (Invitrogen, A18818, 1:200).

Techniques: Analysis, Injection, Immunofluorescence, Knock-Out, Blood/Plasma/Serum Samples, Two Tailed Test

Journal: Bioactive Materials

Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

doi: 10.1016/j.bioactmat.2026.04.004

Figure Lengend Snippet: ID tissue exhibits higher densities of fibroblasts, macrophages, and microvessels compared to SC tissue. Immunofluorescence analysis comparing the expression of Vimentin (a fibroblast marker), CD68 (a macrophage marker), and CD31 (an endothelial cell marker) in ID and SC tissues. For each group, n = 3; data represent mean ± s.d.; ∗P < 0.05 and ∗∗P < 0.01.

Article Snippet: After antigen retrieval and blocking, sections were incubated overnight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206 (Cell Signaling Technology, 24595), and FAPα (ABclonal, A23789 ).

Techniques: Immunofluorescence, Expressing, Marker