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anti ccl2 antibody  (R&D Systems)


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    Structured Review

    R&D Systems anti ccl2 antibody
    Anti Ccl2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ccl2/Canine+CCL2%2FJE%2FMCP-1+Antibody/pm41910735-67-17-19
    Average 90 stars, based on 8 article reviews
    anti ccl2 antibody - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Epigenetic erosion of H4K20me1 induced by inflammation drives aged stem cell ferroptosis.
    Article Snippet: For Gpx4 overexpression, we used Gpx4 (BC106147) Mouse Tagged ORF Clone (cat. no. MG201418; OriGene).

    Recombinant:

    Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells.
    Article Snippet: 16hrs before the assay, 300,000MSCs were seeded per well of a 24- well plate (Corning, 3398, 3μm pore size) without the insert in 500μL cRPMI or 500μL MSC-CM was added per well without the insert. .. 1hr before the addition of monocytes 2μg/mL anti-CCL2 (R&D Systems, MAB679) or 75ng/mL recombinant CCL2 (ThermoFisher, 300-04) were added where indicated. ..

    Article Title: Development and validation of a potency assay matrix for optimized and consistent manufacture of clinical mesenchymal stem/stromal cells
    Article Snippet: 16hrs before the assay, 300,000 MSCs were seeded per well of a 24-well plate (Corning, 3398, 3μm pore size) without the insert in 500μL cRPMI or 500μL MSC-CM was added per well without the insert. .. 1hr before the addition of monocytes 2μg/mL anti-CCL2 (R&D Systems, MAB679) or 75ng/mL recombinant CCL2 (ThermoFisher, 300-04) were added where indicated. ..

    Blocking Assay:

    Article Title: Crosstalk of human coronary perivascular adipose-derived stem cells with vascular cells: role of tissue factor.
    Article Snippet: The coronary perivascular adipose tissue (cPVAT) has been associated to the burden of cardiovascular risk factors and to the underlying vessel atherosclerotic plaque severity.. Although the “outside to inside” hypothesis of PVAT-derived-adipokine regulation of vessel function is currently accepted, whether the resident mesenchymal stem cells (ASCs) in PVAT have a regulatory role on the underlying vascular arterial smooth muscle cells (VSMCs) is not known.. Here, we investigated the interactions between resident PVAT-ASCs and VSMCs.

    Binding Assay:

    Article Title: Crosstalk of human coronary perivascular adipose-derived stem cells with vascular cells: role of tissue factor.
    Article Snippet: The coronary perivascular adipose tissue (cPVAT) has been associated to the burden of cardiovascular risk factors and to the underlying vessel atherosclerotic plaque severity.. Although the “outside to inside” hypothesis of PVAT-derived-adipokine regulation of vessel function is currently accepted, whether the resident mesenchymal stem cells (ASCs) in PVAT have a regulatory role on the underlying vascular arterial smooth muscle cells (VSMCs) is not known.. Here, we investigated the interactions between resident PVAT-ASCs and VSMCs.

    Western Blot:

    Article Title: Crosstalk of human coronary perivascular adipose-derived stem cells with vascular cells: role of tissue factor.
    Article Snippet: The coronary perivascular adipose tissue (cPVAT) has been associated to the burden of cardiovascular risk factors and to the underlying vessel atherosclerotic plaque severity.. Although the “outside to inside” hypothesis of PVAT-derived-adipokine regulation of vessel function is currently accepted, whether the resident mesenchymal stem cells (ASCs) in PVAT have a regulatory role on the underlying vascular arterial smooth muscle cells (VSMCs) is not known.. Here, we investigated the interactions between resident PVAT-ASCs and VSMCs.

    Article Title: Integrated analysis of single-cell and bulk RNA-seq data identifies NR4A1-associated macrophages in immunoglobulin A nephropathy.
    Article Snippet: Immunoglobulin A nephropathy (IgAN), the most prevalent primary glomerulonephritis worldwide, is characterized by chronic renal inflammation and progressive decline toward end-stage renal disease.. In this study, we integrated single-cell and bulk RNA sequencing approaches to systematically elucidate the role of macrophages in IgAN pathogenesis.. Single-cell transcriptomic analysis identified 171 macrophage-associated differentially expressed genes and revealed two distinct molecular subtypes with divergent immune and metabolic signatures.

    Immunohistochemistry:

    Article Title: Integrated analysis of single-cell and bulk RNA-seq data identifies NR4A1-associated macrophages in immunoglobulin A nephropathy.
    Article Snippet: Immunoglobulin A nephropathy (IgAN), the most prevalent primary glomerulonephritis worldwide, is characterized by chronic renal inflammation and progressive decline toward end-stage renal disease.. In this study, we integrated single-cell and bulk RNA sequencing approaches to systematically elucidate the role of macrophages in IgAN pathogenesis.. Single-cell transcriptomic analysis identified 171 macrophage-associated differentially expressed genes and revealed two distinct molecular subtypes with divergent immune and metabolic signatures.

    Transfection:

    Article Title: Epigenomic analyses identify FOXM1 as a key regulator of anti-tumor immune response in esophageal adenocarcinoma
    Article Snippet: Cells were then stained with the following antibodies: Zombie Violet (BioLegend, #423113), CD3 conjugated PerCP/Cyanine (BioLegend, #100217), CD4 conjugated FITC (BioLegend, #100405), CD8 conjugated APC (BioLegend, #100711), CD45 conjugated BV605 (BioLegend, #103155) at 4 °C for 30 min in the dark. .. In a 24-well plate, 400 μL of culture media was collected from YTN5 and YTN16 cells first transfected with FOXM1 siRNA for 24 h, exposure to 20 ng/mL IFNγ (Abcam, #9922) for 24 h, followed by 24-h treatment of (0.5 μg/mL) anti-CCL2 (R&D Systems, #AF-479-SP). .. CD8 + T cells were isolated from the spleens of C57BL/6 mice using the MojoSortTM Mouse CD8 + T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience, #00-4333-57) for 5 min. A 6.5 mm insert with 8.0 μm polycarbonate membranes (COSTAR, #3422) was placed on top of the wells containing culture media.



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    Positive area-staining by antibody in intranasally-treated GHK-Cu mice of both sexes for (A) Synaptophysin, (B) GFAP, (C) <t>MCP-1,</t> (D) PSD-95, and (E) TGF-β. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    R&D Systems anti ccl2 antibody
    Positive area-staining by antibody in intranasally-treated GHK-Cu mice of both sexes for (A) Synaptophysin, (B) GFAP, (C) <t>MCP-1,</t> (D) PSD-95, and (E) TGF-β. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    R&D Systems anti ccl2 mouse monoclonal antibody
    Positive area-staining by antibody in intranasally-treated GHK-Cu mice of both sexes for (A) Synaptophysin, (B) GFAP, (C) <t>MCP-1,</t> (D) PSD-95, and (E) TGF-β. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    Positive area-staining by antibody in intranasally-treated GHK-Cu mice of both sexes for (A) Synaptophysin, (B) GFAP, (C) MCP-1, (D) PSD-95, and (E) TGF-β. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Middle-aged mice treated with GHK-Cu peptide administered intraperitoneally or intranasally show behavioral rescue but divergent hippocampal aging programs

    doi: 10.64898/2026.04.09.717524

    Figure Lengend Snippet: Positive area-staining by antibody in intranasally-treated GHK-Cu mice of both sexes for (A) Synaptophysin, (B) GFAP, (C) MCP-1, (D) PSD-95, and (E) TGF-β. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Following blocking, sections were incubated overnight at 4°C with primary antibodies against synaptophysin (1:250, Invitrogen MA5-16402), PSD95 (1:250, Abcam ab18258), phospho-SMAD2 (1:50, Invitrogen 44-244G), MCP-1 (1:800, Novus NBP1-07035), p21 (1:200, Abcam ab188224), TGF-β (1:50, Abcam ab215715), and GFAP (1:1500, Invitrogen: PA1-10019).

    Techniques: Staining

    Positive area-staining by antibody in intraperitoneally-treated GHK-Cu mice of both sexes for (A) TGF-β, (B) GFAP), (C) MCP-1, (D) p21, (E) Synaptophysin, (F) pSMAD-2, and (G) PSD-95. * P < 0.05, ** P < 0.01, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Middle-aged mice treated with GHK-Cu peptide administered intraperitoneally or intranasally show behavioral rescue but divergent hippocampal aging programs

    doi: 10.64898/2026.04.09.717524

    Figure Lengend Snippet: Positive area-staining by antibody in intraperitoneally-treated GHK-Cu mice of both sexes for (A) TGF-β, (B) GFAP), (C) MCP-1, (D) p21, (E) Synaptophysin, (F) pSMAD-2, and (G) PSD-95. * P < 0.05, ** P < 0.01, **** P < 0.0001.

    Article Snippet: Following blocking, sections were incubated overnight at 4°C with primary antibodies against synaptophysin (1:250, Invitrogen MA5-16402), PSD95 (1:250, Abcam ab18258), phospho-SMAD2 (1:50, Invitrogen 44-244G), MCP-1 (1:800, Novus NBP1-07035), p21 (1:200, Abcam ab188224), TGF-β (1:50, Abcam ab215715), and GFAP (1:1500, Invitrogen: PA1-10019).

    Techniques: Staining