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tricine polyacrylamide gels  (Thermo Fisher)


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    Thermo Fisher tricine polyacrylamide gels
    Tricine Polyacrylamide Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anode+buffer/TRIS-Tricine-SDS+running+buffer+(10X)%2C+cathode+buffer%2C+pH+8%2E3/pm42218136-299-8-11
    Average 95 stars, based on 1 article reviews
    tricine polyacrylamide gels - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: Mitochondrial fission process 1 (MTFP1) controls bioenergetic efficiency and prevents inflammatory cardiomyopathy and heart failure in mice
    Article Snippet: Supernatants were then mixed with G250 sample additive 1% (Invitrogen, BN2004) and resolved on 3-12% Bis-Tris Gels (1.0 mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen, BN2001) and Cathode Buffer Additive added to the anode buffer (InVitroGen, BN2002).

    Article Title: Mitochondrial Fission Process 1 controls inner membrane integrity and protects against heart failure.
    Article Snippet: Supernatants were then mixed with G250 sample additive 1% (Invitrogen, BN2004) and resolved on 3–12% or 4–16% Bis-Tris Gels (1.0mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen, BN2001) and Cathode Buffer Additive added to the anode buffer (InVitroGen, BN2002).

    Article Title: A link between mitochondrial gene expression and life stage morphologies in Trypanosoma cruzi.
    Article Snippet: Briefly, the 720 outer chamber was filled with clear anode buffer (1x NativePAGE Running Buffer, Invitrogen, 721 BN2001) while the inner chamber was filled with dark blue cathode buffer (1x NativePAGE Running 722 Buffer; 1x NativePAGE Cathode Additive, BN2002).

    Article Title: Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines
    Article Snippet: Anode buffer (ABI/Thermo Fisher, Cat. no. 4393927), or equivalent 4.3.7.

    Article Title: Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation
    Article Snippet: The anode buffer (50 mM Bis‐Tris–HCl pH 7.0) and cathode buffer (50 mM Tricine, 15 mM Bis‐Tris–HCl pH 7.0, 0.02% Coomassie G250 (Serva)) were poured into their respective tank compartment and the appropriate amount of protein (55 μg or 5x10 5 parasites) was loaded per lane on a NativePAGETM 4–16% or 3–12% Bis‐Tris Gel (Novex‐ Life technologies).

    Electrofocusing:

    Article Title: Modified DNase and uses thereof
    Article Snippet: Isoelectric Focusing Isoelectric focusing (IEF) analysis was performed using an XCell SureLock Electrophoresis Cell equipped with a Powerpac power supply (BIO-RAD). .. Pre-cast Novex® polyacrylamide IEF gels with a pH range of 3-7 or 3-10, anode buffer, cathode buffer and sample buffer were obtained from Invitrogen. pI protein standards were obtained from SERVA. ..

    Electrophoresis:

    Article Title: Recombinant AcnB, NrdR and RibD of Acinetobacter baumannii and their potential interaction with DNA adenine methyltransferase AamA.
    Article Snippet: .. Electrophoresis was performed in a XCell™ SureLock™ Mini-Cell (Life Technologies™) filled with anode buffer (NativePAGE™ running buffer (20x): 50 ml, deionized water: 950 ml) in the outer buffer chamber and cathode buffer (Dark blue: NativePAGE™ running buffer (20x): 10 ml, NativePAGE™ cathode additive (20x): 10 ml, deionized water: 180 ml) in the inner buffer chamber, for 120 min at 150 V (constant). ..

    Incubation:

    Article Title: Mitochondrial Fission Process 1 controls inner membrane integrity and protects against heart failure
    Article Snippet: .. Lysates were incubated for 1 h at 4 °C and then centrifuged at 30,000 g for 20 min. Supernatants were then mixed with G250 sample additive 1% (Invitrogen, BN2004) and resolved on 3–12% or 4–16% Bis-Tris Gels (1.0 mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen, BN2001) and Cathode Buffer Additive added to the anode buffer (InVitroGen, BN2002). ..



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    APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative <t>native</t> <t>PAGE</t> followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.
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    Image Search Results


    APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative native PAGE followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.

    Journal: PNAS Nexus

    Article Title: APOE3 astrocytes can rescue lipid abnormalities and dystrophic neurites of APOE4 human neurons

    doi: 10.1093/pnasnexus/pgag053

    Figure Lengend Snippet: APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative native PAGE followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.

    Article Snippet: Media samples were mixed with Native Sample Buffer (Bio-Rad, #1610738) supplemented with G-250 Sample Additive (Invitrogen, #BN2004) and run on 4–20% polyacrylamide tris-glycine gels in the absence of sodium dodecyl sulfate, reducing agents or sample boiling, in tris-glycine buffer supplemented with 1:20 native PAGE Cathode Buffer Additive (Invitrogen, #BN2002), at 150 V for 15 min.

    Techniques: Inhibition, Derivative Assay, Clear Native PAGE, Western Blot, Enzyme-linked Immunosorbent Assay