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progranulin grn  (R&D Systems)


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    R&D Systems progranulin grn
    Progranulin Grn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/angiotensinogen/Human+Serpin+A8%2FAngiotensinogen+Antibody/pm41618346-57-14-17
    Average 92 stars, based on 11 article reviews
    progranulin grn - by Bioz Stars, 2026-10
    92/100 stars

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    Incubation:

    Article Title: Expression of angiotensinogen and receptors for angiotensin and prorenin in the monkey and human substantia nigra: an intracellular renin–angiotensin system in the nigra
    Article Snippet: Tissue sections were then pre-incubated in KPBS containing bovine serum albumin (BSA, 1%; Sigma), normal swine or horse serum (4%; Vector Laboratories) and Triton X-100 (0.05%; Sigma) for 60 min at room temperature (RT). .. Tissue sections were subsequently incubated with different polyclonal antibodies raised against major RAS components (Table ): angiotensinogen (goat IgG; 1:200; AF3156, R&D Systems, RD; detects human angiotensinogen), angiotensin (Angiotensin N-10, goat IgG; 1:1,000; sc-7419, Santa Cruz Biotechnology, SC; detects human angiotensinogen, angiotensin I, II and III), AT1R (rabbit IgG; 1:100; sc-579, Santa Cruz Biotechnology), AT2R (rabbit IgG; 1:100; sc-9040, Santa Cruz Biotechnology) and PRR (rabbit IgG; 1:100, ab40790; Abcam) for 48 h at 4°C in a dilution of KPBS–BSA (1%) containing 2% normal swine or horse serum. .. Following primary antibody labeling, tissue sections were first incubated for 60 min at RT in the corresponding biotinylated secondary antibodies (biotinylated swine anti-rabbit IgG, 1:200, Dako; biotinylated horse anti-goat IgG, 1:200, Vector laboratories).

    Article Title: Chymase Inhibition Reduces Infarction and Matrix Metalloproteinase-9 Activation and Attenuates Inflammation and Fibrosis after Acute Myocardial Ischemia/Reperfusion
    Article Snippet: Twenty to 60 μg of total protein were fractionated by 4 to 20, 8 to 16, or 12% SDS polyacrylamide gel electrophoresis (Invitrogen, Carlsbad, CA) and transferred to polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA). .. Each membrane was incubated overnight at 4°C with the following antibodies: angiotensinogen (1:1000; R&D Systems, Minneapolis, MN), chymase (1:200; Abcam plc, Cambridge, UK), MMP-9 (1:500), total eNOS (1:50), phosphor-eNOS (Ser1177; 1:50) (Cell Signaling Technology, Danvers, MA), renin (1:1000), ACE (1:50), angiotensin II type 1 receptor (1:500), angiotensin II type 2 receptor (1:500), vitronectin (1:100), fibronectin (1:100), thrombin (1:100), elastase (1:100), plasminogen (1:100) (Santa Cruz Biotechnology Inc., Santa Cruz, CA), mast cell tryptase (1:100; Leica Microsystems Inc., Bannockbum, IL), and troponin T (1:500; United States Biological, Swampscott, MA). .. The membranes were subsequently incubated for 45 min with the appropriate peroxidase-conjugated secondary antibody (1:1000; Jackson ImmunoResearch Laboratories Inc., West Grove, PA).

    Article Title: Expression of angiotensinogen and receptors for angiotensin and prorenin in the rat and monkey striatal neurons and glial cells.
    Article Snippet: results obtained by immunolabeling and confocal microscopy were confirmed with laser microdissection of striatal neurons and glial cells and detection of mRNA expression by PCR.. The sequence of the resulting PCR products was verified by DNA sequencing.. In addition to the interaction between angiotensin and dopamine receptors in dopaminergic neurons to regulate dopamine release, interaction between angiotensin and dopamine receptors in projection striatal neurons may further modulate the effects of dopamine on the direct and indirect pathways by fine-tuning striatal dopaminergic neurotransmission.

    Article Title: Local Renin-Angiotensin System Activation and Myofibroblast Formation in Graft Versus Host Disease–Associated Conjunctival Fibrosis
    Article Snippet: .. The tissue sections were then incubated with primary antibodies for alpha-smooth muscle actin (α-SMA) (1:100 dilution; Invitrogen, Carlsbad, CA, USA), angiotensinogen (1:50 dilution; R&D Systems, Minneapolis, MN, USA), ACE (1:25 dilution; R&D Systems), AT1 receptors (1:50 dilution; Novus Biologicals, Littleton, CO, USA), and DyLight 594 conjugated tomato lectin (1:200 dilution; Vector Laboratories, Burlingame, CA, USA) for 90 minutes. .. The slides were washed with PBS three times and incubated with Alexa Fluor 488 or Alexa Fluor 647 conjugated secondary antibody (1:500 dilution; Abcam, Cambridge, UK) for 60 minutes.

    Chromatography:

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933‐05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8‐isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156‐05) levels (R&D Systems, Minneapolis, MN). ..

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure.
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933- 05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8- isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156- 05) levels (R&D Systems, Minneapolis, MN). ..

    Mass Spectrometry:

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933‐05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8‐isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156‐05) levels (R&D Systems, Minneapolis, MN). ..

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure.
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933- 05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8- isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156- 05) levels (R&D Systems, Minneapolis, MN). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933‐05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8‐isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156‐05) levels (R&D Systems, Minneapolis, MN). ..

    Article Title: Mechanistic evaluation of ertugliflozin in patients with type 2 diabetes and heart failure.
    Article Snippet: Plasma renin concentrations were measured with a sandwich chemiluminescence immunoassay kit (Catalog No. M0870004217; LIAISON®, DiaSorin S.p.A., Italy) and enzymatic assays, including PRA (Catalog No. MBS495044), ACE (Catalog No. MBS9359476), and ACE2 activity (Catalog No. DY933- 05), were measured using ELISA kits. .. Aldosterone, catecholamines, and urine adenosine were measured using chromatography and mass spectrometry, while ELISA was used to measure urine cGMP (Catalog No. MBS160025), 8- isoprostane (Catalog No. ab175819), NO (Catalog No. KGE001), angiotensin II (Catalog No. MBS703599), and angiotensinogen (Catalog No. DY3156- 05) levels (R&D Systems, Minneapolis, MN). ..

    Membrane:

    Article Title: Chymase Inhibition Reduces Infarction and Matrix Metalloproteinase-9 Activation and Attenuates Inflammation and Fibrosis after Acute Myocardial Ischemia/Reperfusion
    Article Snippet: Twenty to 60 μg of total protein were fractionated by 4 to 20, 8 to 16, or 12% SDS polyacrylamide gel electrophoresis (Invitrogen, Carlsbad, CA) and transferred to polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA). .. Each membrane was incubated overnight at 4°C with the following antibodies: angiotensinogen (1:1000; R&D Systems, Minneapolis, MN), chymase (1:200; Abcam plc, Cambridge, UK), MMP-9 (1:500), total eNOS (1:50), phosphor-eNOS (Ser1177; 1:50) (Cell Signaling Technology, Danvers, MA), renin (1:1000), ACE (1:50), angiotensin II type 1 receptor (1:500), angiotensin II type 2 receptor (1:500), vitronectin (1:100), fibronectin (1:100), thrombin (1:100), elastase (1:100), plasminogen (1:100) (Santa Cruz Biotechnology Inc., Santa Cruz, CA), mast cell tryptase (1:100; Leica Microsystems Inc., Bannockbum, IL), and troponin T (1:500; United States Biological, Swampscott, MA). .. The membranes were subsequently incubated for 45 min with the appropriate peroxidase-conjugated secondary antibody (1:1000; Jackson ImmunoResearch Laboratories Inc., West Grove, PA).



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    Image Search Results


    A workflow of this study (A) Overview of the mechanism of action of N-acetylgalactosamine-small interfering RNA (GalNAc-siRNA) and the seven approved GalNAc-SiRNA drugs collected in this study. (B) Schematic structure of the developed computational model for a GalNAc-siRNA. (C) Schematic workflow of the model informed GalNAc-siRNA development powered by the established computational platform.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: A computational model-powered platform to inform the development of GalNAc-conjugated siRNA therapeutics

    doi: 10.1016/j.omtn.2026.102936

    Figure Lengend Snippet: A workflow of this study (A) Overview of the mechanism of action of N-acetylgalactosamine-small interfering RNA (GalNAc-siRNA) and the seven approved GalNAc-SiRNA drugs collected in this study. (B) Schematic structure of the developed computational model for a GalNAc-siRNA. (C) Schematic workflow of the model informed GalNAc-siRNA development powered by the established computational platform.

    Article Snippet: Finally, the platform was utilized to support the development of an investigational new angiotensinogen ( AGT )-silencing GalNAc-siRNA, termed SAL0132 (developed by Shenzhen Salubris Pharmaceuticals Co., Ltd), from preclinic rats and monkey to clinical studies.

    Techniques: Small Interfering RNA