aml 12 cells  (ATCC)

Journal: Inflammation

Article Title: Hepatocyte-Derived IL-25 Promotes Macrophage Extracellular Trap Formation and Drives Liver Fibrosis Progression

doi: 10.1007/s10753-026-02506-6

Figure Lengend Snippet: Upregulated hepatocyte-derived IL-25 promotes the formation of METs in liver fibrosis. A qPCR analysis of 31 kinds of Interleukins expression in 4 W LFib mice liver tissues, The heatmap and bar graph visually represent the fold changes of IL-25 and other interleukins. ( n = 6 per group). B qPCR analysis of 31 kinds of Interleukins expression in AML-12, stimulated with culture medium containing 0.2% CCl₄ for 12 h. The heatmap and bar graph visually represent the fold changes of IL-25 and other interleukins. ( n = 6 per group). C Double immunofluorescence staining of mouse liver tissue sections using HNF4α and Il-25 antibodies, with DAPI for nuclear counterstaining with the semi-quantitative results of the processed images from each group, yellow arrows indicate hepatocyte-derived IL-25 (HNF4α⁺Il-25⁺). ( n = 6 per group). Mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Student’s t-test

Article Snippet: The murine immortalized macrophage cell line RAW 264.7 (Cat. No. CL-0190, Wuhan, China) and AML-12 (alpha mouse liver 12) cell line was purchased from ProCell (Cat. No. CL-0602, Wuhan, China).

Techniques: Derivative Assay, Expressing, Double Immunofluorescence Staining

Journal: World Journal of Stem Cells

Article Title: GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

doi: 10.4252/wjsc.v18.i2.112940

Figure Lengend Snippet: Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in thapsigargin-treated AML-12 cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.

Article Snippet: Human hepatocellular carcinoma HepG2 cells and mouse hepatocyte AML-12 cells were purchased from the American Type Culture Collection and authenticated via short tandem repeat profiling.

Techniques: Expressing, Cell Counting, Western Blot, Control