alphascreen igg (Revvity)
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Revvity
alphascreen igg
Alphascreen Igg, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphascreen+surefire+kit/AlphaScreen+SureFire+HV+IgG+(Protein+A)+Detection+Kit/pmc10290147-213-6-12
Average 91 stars, based on 15 article reviews
Alphascreen Igg, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alphascreen+surefire+kit/AlphaScreen+SureFire+HV+IgG+(Protein+A)+Detection+Kit/pmc10290147-213-6-12
Average 91 stars, based on 15 article reviews
alphascreen igg - by Bioz Stars,
2026-10
91/100 stars
Images
1) Product Images from "GATS tag system is compatible with biotin labelling methods for protein analysis"
Article Title: GATS tag system is compatible with biotin labelling methods for protein analysis
Journal: Scientific Reports
doi: 10.1038/s41598-023-36858-y
Figure Legend Snippet: Identification of anti-GAMA rabbit monoclonal antibodies. ( a ) A simplified cellular model of Plasmodium falciparum . ( b ) Domain information on the GAMA protein and the location of GAMA-F. ( c ) Image showing immunoblotting evaluation of the binding of antibodies to GAMA-F and Ripr-F (control) synthesized using a wheat cell-free protein synthesis system. Antibodies are unpurified supernatants produced in Expi293F cells. ( d ) In vitro interaction assay of rabbit monoclonal antibodies clone (Ra) and GAMA-F. Ripr-F is used as control. Antibodies are unpurified supernatants produced in Expi293F cells. All AlphaScreen signals are raw luminescent signals in the AlphaScreen-based biochemical assay. Error bars denote the standard deviation (independent experiments; n = 3). ( e ) Evaluation of antibody specificity with lysates of P. falciparum . Antibodies are produced in Expi293F cells and purified by protein G Sepharose 4 Fast Flow (GE Healthcare).
Techniques Used: Bioprocessing, Western Blot, Binding Assay, Control, Synthesized, Produced, In Vitro, Amplified Luminescent Proximity Homogenous Assay, Standard Deviation, Purification
Figure Legend Snippet: Broad epitope determination of Ra3, Ra9, and Ra13. ( a ) Diagram representing the GAMA-F fragment for epitope determination. ( b ) Identification of epitope region using A, B, and C GAMA-F fragments. These fragments were fused to the N-terminus of GST-TEV-bls protein and synthesized using a wheat germ cell-free system. Binding between the fusion protein and Ra3, Ra9, and Ra13 antibodies was detected by AlphaScreen. ( c ) Identification of epitope region using smaller segmented fragments of A, B, and C. ( d ) Diagram representing the lysine residues of the GAMA-F fragment.
Techniques Used: Synthesized, Binding Assay, Amplified Luminescent Proximity Homogenous Assay
Figure Legend Snippet: The minimal amino acid epitope determination of Ra3. ( a ) Immunoblot analysis of deletion mutants to identify the minimum Ra48 epitope sequence. ( b ) Kinetics assay of the GATS tag and anti-GATS antibody. Anti-GATS antibody was captured on a protein G-immobilized Biacore sensorchip at 1500 RU. Purified FLAG-GST-GATS protein was then injected for 1600 s as analyte. Black lines represent a global fit of a 1:1 interaction model to each kinetic data set. ( c ) Pointing out antibody concentrations in AlphaScreen. The GATS tag was fused to the N-terminus of GST-TEV-bls protein and synthesized using a wheat germ cell-free system.
Techniques Used: Western Blot, Sequencing, Purification, Injection, Amplified Luminescent Proximity Homogenous Assay, Synthesized
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