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mouse a fetoprotein quantikine elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems mouse a fetoprotein quantikine elisa kit
    Mouse A Fetoprotein Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpha+fetoprotein/Mouse+alpha-Fetoprotein%2FAFP+Quantikine+ELISA+Kit/pmc13068069-473-8-13
    Average 94 stars, based on 37 article reviews
    mouse a fetoprotein quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Article Snippet: Nuclei were counterstained with 4′,6′diamidino-2-phenylidole (DAPI) (Vector Laboratories Inc., Burlingame, CA). .. Pluripotency was verified with in vitro pluripotency assay by spontaneous differentiation as embryonic bodies , followed by immunofluorescence analysis for alpha-smooth muscle actin (SMA, 1:400, R&D Systems, MAB1420) for mesoderm, alpha-fetoprotein (AFP, 1:200, R&D Systems MAB1369) for endoderm, and OTX2 (1:200, R&D Systems, AF1979) for ectoderm. .. Karyotyping was performed at Finnish Microarray and Sequencing Centre (FMSC), Turku Centre for Biotechnology with the KaryoLite BoBs assay (Perkin Elmer).

    Pluripotency Assay:

    Article Title: Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Article Snippet: Nuclei were counterstained with 4′,6′diamidino-2-phenylidole (DAPI) (Vector Laboratories Inc., Burlingame, CA). .. Pluripotency was verified with in vitro pluripotency assay by spontaneous differentiation as embryonic bodies , followed by immunofluorescence analysis for alpha-smooth muscle actin (SMA, 1:400, R&D Systems, MAB1420) for mesoderm, alpha-fetoprotein (AFP, 1:200, R&D Systems MAB1369) for endoderm, and OTX2 (1:200, R&D Systems, AF1979) for ectoderm. .. Karyotyping was performed at Finnish Microarray and Sequencing Centre (FMSC), Turku Centre for Biotechnology with the KaryoLite BoBs assay (Perkin Elmer).

    Immunofluorescence:

    Article Title: Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Article Snippet: Nuclei were counterstained with 4′,6′diamidino-2-phenylidole (DAPI) (Vector Laboratories Inc., Burlingame, CA). .. Pluripotency was verified with in vitro pluripotency assay by spontaneous differentiation as embryonic bodies , followed by immunofluorescence analysis for alpha-smooth muscle actin (SMA, 1:400, R&D Systems, MAB1420) for mesoderm, alpha-fetoprotein (AFP, 1:200, R&D Systems MAB1369) for endoderm, and OTX2 (1:200, R&D Systems, AF1979) for ectoderm. .. Karyotyping was performed at Finnish Microarray and Sequencing Centre (FMSC), Turku Centre for Biotechnology with the KaryoLite BoBs assay (Perkin Elmer).

    Incubation:

    Article Title: Mice lacking RAP1 show early onset and higher rates of DEN-induced hepatocellular carcinomas in female mice
    Article Snippet: .. The liver slides were incubated with the primary antibodies: goat polyclonal to alpha-fetoprotein (R&D systems), mouse monoclonal to phospho-Histone H2AX (ser139) (JBW301, Millipore), rabbit monoclonal to Ki-67 (D3B5, Cell Signaling) or rabbit polyclonal to C3 cleaved-caspase 3 (Asp175) (Cell Signaling); the lung slides were incubated with the primary antibody: mouse monoclonal to human hepatocytes (OCH1E5, DAKO). .. Slides were then incubated with secondary antibodies conjugated with peroxidase from DAKO.

    Article Title: Method for expanding stemness and differentiation potential of pluripotent cells
    Article Snippet: Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). .. Antigen retrival was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrival buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were incubated with primary antibodies against Nanog (Cell Signalling Biotechnology, 8822), cytokeratin 8 (CK8; CNIO Monoclonal Antibodies Core Unit, AM-TROMA I), GFP (Roche, 11814460001), Sox2 (Cell Signaling Technology, 3728), alpha-fetoprotein (AFP; R&D Systems, AF5369), Oct4 (Santa Cruz Biotechnology, sc-9081), KI-67 (Master Diagnostica, 0003110QD), Nestin (Millipore MAB353), Cd31 (Abcam), Cd34 (ABCAM ab8158), Cd73 (Cell Signaling Technology), Collagenase Type I (Rockland), Gata4 (Santa Cruz Biotechnology, sc-1237), Insulin (Dako A0564), Smooth muscle actin (Thermo Scientific RB-9010-PO), skeletal actin (Dako, M0635), and Ter119 (LY-76; BD Bioscience, 550565) were used. ..

    Article Title: A novel microRNA-based strategy to expand the differentiation potency of stem cells
    Article Snippet: Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). .. Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrieval buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were incubated with primary antibodies against cytokeratin 8 (CK8; CNIO Monoclonal Antibodies Core Unit, AM-TROMA I), GFP (Roche, 11814460001), Sox2 (Cell Signaling Technology, 3728), alpha-fetoprotein (AFP; R&D Systems, AF5369), Oct4 (Santa Cruz Biotechnology, sc-9081), Cd34 (ABCAM ab8158), Gata4 (Santa Cruz Biotechnology, sc-1237), or Ter119 (LY-76; BD Bioscience, 550565) (Supplementary Table 8). .. Secondary antibodies were conjugated with horseradish peroxidase (OmniRabbit, Ventana, Roche) and the immunohistochemical reaction was developed using 3,30-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (Chromomap DAB, Ventana, Roche or DAB solution, Dako) and nuclei were counterstained with Carazzi’s hematoxylin.

    Bioprocessing:

    Article Title: Method for expanding stemness and differentiation potential of pluripotent cells
    Article Snippet: Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). .. Antigen retrival was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrival buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were incubated with primary antibodies against Nanog (Cell Signalling Biotechnology, 8822), cytokeratin 8 (CK8; CNIO Monoclonal Antibodies Core Unit, AM-TROMA I), GFP (Roche, 11814460001), Sox2 (Cell Signaling Technology, 3728), alpha-fetoprotein (AFP; R&D Systems, AF5369), Oct4 (Santa Cruz Biotechnology, sc-9081), KI-67 (Master Diagnostica, 0003110QD), Nestin (Millipore MAB353), Cd31 (Abcam), Cd34 (ABCAM ab8158), Cd73 (Cell Signaling Technology), Collagenase Type I (Rockland), Gata4 (Santa Cruz Biotechnology, sc-1237), Insulin (Dako A0564), Smooth muscle actin (Thermo Scientific RB-9010-PO), skeletal actin (Dako, M0635), and Ter119 (LY-76; BD Bioscience, 550565) were used. ..

    Article Title: A novel microRNA-based strategy to expand the differentiation potency of stem cells
    Article Snippet: Consecutive sections were stained with hematoxylin and eosin (H&E) or subjected to immunohistochemistry using automated immunostaining platforms (Ventana Discovery XT, Roche or Autostainer Plus Link 48). .. Antigen retrieval was first performed with high or low pH buffer depending on the primary antibody (CC1m, Roche or low pH antigen retrieval buffer, Dako), endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were incubated with primary antibodies against cytokeratin 8 (CK8; CNIO Monoclonal Antibodies Core Unit, AM-TROMA I), GFP (Roche, 11814460001), Sox2 (Cell Signaling Technology, 3728), alpha-fetoprotein (AFP; R&D Systems, AF5369), Oct4 (Santa Cruz Biotechnology, sc-9081), Cd34 (ABCAM ab8158), Gata4 (Santa Cruz Biotechnology, sc-1237), or Ter119 (LY-76; BD Bioscience, 550565) (Supplementary Table 8). .. Secondary antibodies were conjugated with horseradish peroxidase (OmniRabbit, Ventana, Roche) and the immunohistochemical reaction was developed using 3,30-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (Chromomap DAB, Ventana, Roche or DAB solution, Dako) and nuclei were counterstained with Carazzi’s hematoxylin.



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