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alcian blue stain kit  (Vector Laboratories)


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    Vector Laboratories alcian blue stain kit
    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Alcian Blue Stain Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alcian+blue+kit/Alcian+Blue+(pH+2%2E5)+Stain+Kit/pmc13114135-135-19-27
    Average 95 stars, based on 96 article reviews
    alcian blue stain kit - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis"

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    Journal: Gut Microbes

    doi: 10.1080/19490976.2026.2662638

    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
    Figure Legend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Techniques Used: Staining, Western Blot, Quantitative RT-PCR, Expressing

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    Staining:

    Article Title: Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous Transplantation
    Article Snippet: Differentiation procedures for chondrogenesis were followed in triplicate according to manufacturer protocols (StemPro Chondrogenesis Kit; Gibco), with the following exceptions to optimize for pADMSC differentiation [ ]: Micromass cultures were created using 15 μL of pADMSCs suspended at 1×10 7 cells/mL vs 5 μL, as suggested by the manufacturer Incubation temperature for differentiation was 39°C for 1 set of pADMSC cultures and 37°C for all others. .. Alcian blue staining was performed by using components from the Alcian blue kit (Vector Laboratories; Burlingame, California) [ ]. .. Briefly, micromass cultures were fixed with 10% formalin for 30 minutes and washed twice with 2 mL of double-deionized water.

    Article Title: Evidence of an allostatic response by intestinal tissues following induction of joint inflammation
    Article Snippet: Sagittal sections from knee joints were stained with Hematoxylin/Eosin, anti-CD68 (Abcam, Ab283654 ; 1:100) or anti-CD4 Abcam, Ab237722 1:2000). .. Sections of intestinal Swiss rolls were stained using the Alcian Blue Kit (Vector Labs, H-3501), anti-CD68 (1:100), anti-CD4 (1:2000), anti-CD8 (Abcam, Ab237709 , 1:500) or anti-Ki67 (Invitrogen, MA5–14520; 1:100), Invitrogen, MA5–14520) or the following biotinylated lectins (all from Vector Labs), Griffonia Bandeiraea Simplidifolia Lectin I, (GSL I, B-1105–2), Maackia Amurensis Lectin II (MAL II, B-1265–1) and Ulex Europaeus Agglutinin I (UEA, B-1065–2). .. Prior to histochemical staining, antigen retrieval was performed using Tris/EDTA (Abcam, ab93684), and sections were blocked with normal goat serum (Vector Laboratories, S-1000).



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    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) <t>Alcian</t> <t>blue</t> staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).
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    Image Search Results


    Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Gut Microbes

    Article Title: Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitis

    doi: 10.1080/19490976.2026.2662638

    Figure Lengend Snippet: Monomeric Ent, 2, 3-DHBA, but not 2, 5-DHBA, restores mucosal integrity and mitochondrial homeostasis in DSS-induced colitis. Acute colitis was induced in mice as in Figure 7. After the cessation of DSS (recovery phase) mice were given either 2, 3-DHBA or 2, 5-DHBA (500 µg/mouse per day) for 7 d and euthanized. (A) Representative H&E-stained colon sections showing epithelial damage, crypt loss, and inflammatory cells ( n = 5/group). (B) Alcian blue staining for goblet cells in the colon (C) Immunoblot analysis of tight junction proteins (occludin and claudin-1) in colonic tissue. (D) qRT-PCR analysis of genes involved in mitochondrial biogenesis peroxisome proliferator activated receptor gamma coactivator 1-alpha ( PGC-1α ) and deiodinase type 2 ( Dio2) .(E) Colonic expression of antioxidant genes (Superoxide dismutase 2 (SOD2) , glutathione peroxidase (Gpx) , and nuclear factor erythroid 2-related factor 2 (Nrf2) (F) Immunoblot showing protein involved in mitochondrial dynamics uncoupling protein 1 (Ucp1), mitofusin 1 (Mfn1), and optic atrophy type 1 (Opa1). Data is presented as mean ± SEM ( n = 5). Statistical comparisons were performed using one-way ANOVA with Tukey’s post hoc test (* p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: Alcian blue staining was performed to visualize acidic mucopolysaccharides and glycosaminoglycans in goblet cells of colon sections using the Alcian Blue Stain Kit (pH 2.5, Cat# H-3501, Vector Laboratories), following the manufacturer’s instructions.

    Techniques: Staining, Western Blot, Quantitative RT-PCR, Expressing

    V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

    Journal: Frontiers in Pharmacology

    Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

    doi: 10.3389/fphar.2026.1803816

    Figure Lengend Snippet: V. parvula exacerbates colonic mucus barrier damage in DSS-induced colitis mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A,B) AB-PAS staining in mouse colon. (C) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). Data are expressed as mean ± S.D. (n = 6). #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the DSV group. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the DSS group.

    Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

    Techniques: Staining

    V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

    Journal: Frontiers in Pharmacology

    Article Title: Modified gegen qinlian decoction ameliorates Veillonella parvula -exacerbated ulcerative colitis via restoration of intestinal mucosal barrier function

    doi: 10.3389/fphar.2026.1803816

    Figure Lengend Snippet: V. parvula exacerbates colonic barrier damage in DSS-induced colitis pseudo-germ-free mice, while the MGQD ameliorates this V. parvula -exacerbated damage. (A) The AB-PAS staining in mouse colon. (B) The IF staining of mouse colon for Muc2 (red) and DAPI (blue). (C) The Western blot of mouse colon. The data are expressed as the means ± S.D. (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the VD group.

    Article Snippet: Hematoxylin and eosin (H&E) and Alcian Blue/Periodic Acid-Schiff (AB/PAS) staining kits were provided by Servicebio (Wuhan, China, Batch number:G1049).

    Techniques: Staining, Western Blot