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6202 anti mouse ifn  (R&D Systems)


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    R&D Systems 6202 anti mouse ifn
    6202 Anti Mouse Ifn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6202 anti mouse ifn/product/R&D Systems
    Average 93 stars, based on 11 article reviews
    6202 anti mouse ifn - by Bioz Stars, 2026-04
    93/100 stars

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    UBXN3B restricted CHIKV infection in an IFN-I-independent manner. (A and B) Bone marrow-derived mouse dendritic cells were infected with CHIKV/ZIKV at a multiplicity of infection (MOI) of 1. Quantitative RT-PCR (qPCR) quantification of (A) viral RNA and (B) immune gene mRNA. Each symbol is one mouse. The small horizontal line is the median of the result. (C) Viral titers (PFU/mL) in the supernatants of mouse embryonic fibroblast (MEFs) infected with CHIKV (MOI = 0.5). Bar, mean ± SEM (N = 3). qPCR quantification of (D) ISG mRNA and (E) CHIKV RNA. MEFs were treated with an anti-IFNAR1 antibody or control IgG (8 μg/mL) for 2 h and then infected with CHIKV (MOI = 0.5) in the presence of antibodies for 24 h (N = 6 biological repeats). *, P < 0.05; **, P < 0.01 (two-tailed Student's t test). The results are representative of 3 independent experiments.

    Journal: mBio

    Article Title: UBXN3B Controls Immunopathogenesis of Arthritogenic Alphaviruses by Maintaining Hematopoietic Homeostasis

    doi: 10.1128/mbio.02687-22

    Figure Lengend Snippet: UBXN3B restricted CHIKV infection in an IFN-I-independent manner. (A and B) Bone marrow-derived mouse dendritic cells were infected with CHIKV/ZIKV at a multiplicity of infection (MOI) of 1. Quantitative RT-PCR (qPCR) quantification of (A) viral RNA and (B) immune gene mRNA. Each symbol is one mouse. The small horizontal line is the median of the result. (C) Viral titers (PFU/mL) in the supernatants of mouse embryonic fibroblast (MEFs) infected with CHIKV (MOI = 0.5). Bar, mean ± SEM (N = 3). qPCR quantification of (D) ISG mRNA and (E) CHIKV RNA. MEFs were treated with an anti-IFNAR1 antibody or control IgG (8 μg/mL) for 2 h and then infected with CHIKV (MOI = 0.5) in the presence of antibodies for 24 h (N = 6 biological repeats). *, P < 0.05; **, P < 0.01 (two-tailed Student's t test). The results are representative of 3 independent experiments.

    Article Snippet: The goat anti-mouse IFNAR1 (number AF30369) was purchased from R&D Systems (Minneapolis, MN 55413, USA), rabbit anti-GAPDH (Clone D16H11, number 5174), anti-β-Actin (Clone 13E5, number 4970) and STING (Clone D2P2F, number 13647) were from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Infection, Derivative Assay, Quantitative RT-PCR, Control, Two Tailed Test