Journal: Preventive Nutrition and Food Science
Article Title: Flavonoids of Cuscuta chinensis Enhance Osteogenic Differentiation in Dental Pulp Stem Cells via the Regulation of Wnt/β-Catenin Signaling
doi: 10.3746/pnf.2025.243
Figure Lengend Snippet: Isolation, culture, and characterization of human dental pulp stem cells (HDPSCs). (A) HDPSCs (P0) were observed using an optical microscope on day 7 (×100). (B) HDPSCs (P1) were observed using an optical microscope on day 2 (×40). (C) HDPSCs (P3) were observed using an optical microscope on day 2 (×40). (D) HDPSC phenotype was analyzed using flow cytometry. (E) Adipogenic differentiation of HDPSCs was evaluated using Oil red O staining at day 21 (×200). (F) Mineralized matrix formation in HDPSCs was ascertained using Alizarin red S staining at day 21 (×100).
Article Snippet: Adipogenic differentiation medium (Cat. no. PD-016) was bought from Procell; Oil red O (ORO) solution from Beijing Solarbio Science & Technology Co., Ltd., TFCC from DESITE, and the Wnt inhibitor-XAV-939 (Cat. no. HY-15147)-was from MCE.
Techniques: Isolation, Microscopy, Flow Cytometry, Staining