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ad-gfp  (Vector Biolabs)


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    Structured Review

    Vector Biolabs ad-gfp
    Ad Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad+gfp/Ad-GFP/custom%401060%4042532545
    Average 96 stars, based on 370 article reviews
    ad-gfp - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Control:

    Article Title: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity
    Article Snippet: Evaluations of exercise capacity and blood metabolites during endurance running Mice ran on single-lane rodent treadmills equipped with individual shock grates to encourage running (Columbus Instruments). .. Evaluation of mitochondria morphology and rescue experiments in C2C12 cells Control and AnkB220-KO C2C12 cells were transduced for 60 hr with Ad-GFP (Vector Biolabs, 1060) or Ad-AnkB220-GFP adenoviruses (1 × 10^10 PFU/ml), packed and purified by Vector Biolabs. .. Cells were incubated with 250nM Mitotracker CMXRos (Invitrogen, M7512) for 15min at 37 °C, washed with PBS, fixed for 15min with 4% PFA, and imaged in PBS.

    Article Title: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity
    Article Snippet: .. Control and AnkB220-KO C2C12 cells were transduced for 60 hr with Ad-GFP (Vector Biolabs, 1060) or Ad-AnkB220-GFP adenoviruses (1 × 10^10 PFU/ml), packed and purified by Vector Biolabs. .. Cells were incubated with 250 nM Mitotracker CMXRos (Invitrogen, M7512) for 15 min at 37 °C, washed with PBS, fixed for 15 min with 4% PFA, and imaged in PBS.

    Purification:

    Article Title: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity
    Article Snippet: Evaluations of exercise capacity and blood metabolites during endurance running Mice ran on single-lane rodent treadmills equipped with individual shock grates to encourage running (Columbus Instruments). .. Evaluation of mitochondria morphology and rescue experiments in C2C12 cells Control and AnkB220-KO C2C12 cells were transduced for 60 hr with Ad-GFP (Vector Biolabs, 1060) or Ad-AnkB220-GFP adenoviruses (1 × 10^10 PFU/ml), packed and purified by Vector Biolabs. .. Cells were incubated with 250nM Mitotracker CMXRos (Invitrogen, M7512) for 15min at 37 °C, washed with PBS, fixed for 15min with 4% PFA, and imaged in PBS.

    Article Title: Ankyrin-B modulates mitochondrial fission in skeletal muscle and is required for optimal endurance exercise capacity
    Article Snippet: .. Control and AnkB220-KO C2C12 cells were transduced for 60 hr with Ad-GFP (Vector Biolabs, 1060) or Ad-AnkB220-GFP adenoviruses (1 × 10^10 PFU/ml), packed and purified by Vector Biolabs. .. Cells were incubated with 250 nM Mitotracker CMXRos (Invitrogen, M7512) for 15 min at 37 °C, washed with PBS, fixed for 15 min with 4% PFA, and imaged in PBS.

    Injection:

    Article Title: IL-9 signaling redirects CAR T cell fate toward CD8 + memory and CD4 + cycling states, enhancing antitumor efficacy.
    Article Snippet: Article IL-9 signaling redirects CAR T cell fate toward CD8+ memory and CD4+ cycling states, enhancing antitumor efficacy

    Article Title: PACAP inhibits sepsis-associated acute lung injury by inhibiting the Sp1/AQP1 pathway.
    Article Snippet: Sepsis-induced acute lung injury (ALI) represents a severe pathological state marked by uncontrolled inflammation, redox imbalance, and alveolar-capillary barrier breakdown.. Here, we evaluated the therapeutic potential of pituitary adenylate cyclase-activating polypeptide (PACAP) in a murine sepsis-ALI model. PACAP treatment notably ameliorated histological damage, reduced oxidative stress biomarkers, and mitigated inflammatory processes, including neutrophil accumulation and pro-inflammatory cytokine release.. Molecular analysis revealed PACAP-mediated downregulation of Aquaporin-1 (AQP1) and specificity protein 1 (Sp1), key regulators of alveolar fluid homeostasis and inflammatory signaling.

    Negative Control:

    Article Title: ANTXR1 blockade enhances cardiac function in preclinical models of heart failure
    Article Snippet: .. Ad-GFP (Vector Biolabs, cat. no. 1060) was used as a negative control. .. For rescue experiments, full-length human ANTXR1 and a cytosolic domain deletion mutant (Δ355–564 aa, ANTXR1-CytDel) were cloned into pLenti vector (Addgene, cat. no. 17448).

    Article Title: ANTXR1 blockade enhances cardiac function in preclinical models of heart failure.
    Article Snippet: .. Ad-GFP (Vector Biolabs, cat. no. 1060) was used as a negative control. .. For rescue experiments, full-length human ANTXR1 and a cytosolic domain deletion mutant (Δ355–564 aa, ANTXR1-CytDel) were cloned into pLenti vector (Addgene, cat. no. 17448).

    Mouse Assay:

    Article Title: PACAP inhibits sepsis-associated acute lung injury by inhibiting the Sp1/AQP1 pathway.
    Article Snippet: Sepsis-induced acute lung injury (ALI) represents a severe pathological state marked by uncontrolled inflammation, redox imbalance, and alveolar-capillary barrier breakdown.. Here, we evaluated the therapeutic potential of pituitary adenylate cyclase-activating polypeptide (PACAP) in a murine sepsis-ALI model. PACAP treatment notably ameliorated histological damage, reduced oxidative stress biomarkers, and mitigated inflammatory processes, including neutrophil accumulation and pro-inflammatory cytokine release.. Molecular analysis revealed PACAP-mediated downregulation of Aquaporin-1 (AQP1) and specificity protein 1 (Sp1), key regulators of alveolar fluid homeostasis and inflammatory signaling.



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    Image Search Results


    Primary cilia are required for GLP-1-potentiated insulin secretion. ( A ) Dynamic insulin secretion from perifused mouse islets. Wild-type (WT, black) and β-cell-specific cilia knockout (βCKO, red) islets were sequentially exposed to 2 mM glucose (2G), 16 mM glucose (16G), 20 nM liraglutide (Lira), and 30 mM KCl as indicated. n = 6 replicates of 50 islets per genotype from 5 mice. ∗p < 0.05, two-way ANOVA with Sidak's multiple comparisons test. ( B ) Quantitative analysis of secretion traces: area under the curve (AUC) during initial glucose stimulation (minutes 10–30), liraglutide stimulation (minutes 30–50), KCl depolarization (minutes 60–70), and total AUC of the entire perifusion (minutes 0–70). βCKO islets secreted significantly less insulin in response to glucose and liraglutide but showed no defect in KCl-induced secretion. Data are mean ± SEM. ∗∗p < 0.01; ns, not significant, unpaired student's t-test. ( C-D ) IFT88 knockdown impairs GLP-1-augmented secretion in human islets. Static glucose-stimulated insulin secretion (GSIS) assays from islets of three male ( C ) and three female ( D ) donors. Islets transduced with control (Ctl, white) or IFT88 -targeting shRNA (IFT88 KD, red) were incubated at 1 mM glucose (1G), 11 mM glucose (11G), and 11G + 100 nM liraglutide (Lira). Donor ages are indicated. IFT88 KD significantly reduced liraglutide-potentiated insulin secretion. Data are mean ± SEM of triplicate samples per donor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant; one-way ANOVA with Tukey's multiple comparisons test.

    Journal: Molecular Metabolism

    Article Title: Primary cilia regulate GLP-1 signaling in pancreatic β cells

    doi: 10.1016/j.molmet.2026.102357

    Figure Lengend Snippet: Primary cilia are required for GLP-1-potentiated insulin secretion. ( A ) Dynamic insulin secretion from perifused mouse islets. Wild-type (WT, black) and β-cell-specific cilia knockout (βCKO, red) islets were sequentially exposed to 2 mM glucose (2G), 16 mM glucose (16G), 20 nM liraglutide (Lira), and 30 mM KCl as indicated. n = 6 replicates of 50 islets per genotype from 5 mice. ∗p < 0.05, two-way ANOVA with Sidak's multiple comparisons test. ( B ) Quantitative analysis of secretion traces: area under the curve (AUC) during initial glucose stimulation (minutes 10–30), liraglutide stimulation (minutes 30–50), KCl depolarization (minutes 60–70), and total AUC of the entire perifusion (minutes 0–70). βCKO islets secreted significantly less insulin in response to glucose and liraglutide but showed no defect in KCl-induced secretion. Data are mean ± SEM. ∗∗p < 0.01; ns, not significant, unpaired student's t-test. ( C-D ) IFT88 knockdown impairs GLP-1-augmented secretion in human islets. Static glucose-stimulated insulin secretion (GSIS) assays from islets of three male ( C ) and three female ( D ) donors. Islets transduced with control (Ctl, white) or IFT88 -targeting shRNA (IFT88 KD, red) were incubated at 1 mM glucose (1G), 11 mM glucose (11G), and 11G + 100 nM liraglutide (Lira). Donor ages are indicated. IFT88 KD significantly reduced liraglutide-potentiated insulin secretion. Data are mean ± SEM of triplicate samples per donor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant; one-way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: IFT88 knockdown in human islets: Healthy non-diabetic human islets were transduced with adenoviral vectors encoding either GFP-tagged shRNA targeting human IFT88 (Ad-GFP-h-IFT88-shRNA) or scrambled control (Ad-GFP-U6-scrmb-shRNA, #1122N; Vector Biolabs).

    Techniques: Knock-Out, Knockdown, Transduction, Control, shRNA, Incubation