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null adenovirus  (Vector Biolabs)


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    Structured Review

    Vector Biolabs null adenovirus
    A) AML12 cells transduced with ATGL or GFP control <t>adenoviruses</t> were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
    Null Adenovirus, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad+cmv+null/Ad-CMV-Null/bio_rxiv__64898__2026__04__03__716381-177-22-27
    Average 96 stars, based on 134 article reviews
    null adenovirus - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "ATGL-catalyzed lipid catabolism promotes DNA repair"

    Article Title: ATGL-catalyzed lipid catabolism promotes DNA repair

    Journal: bioRxiv

    doi: 10.64898/2026.04.03.716381

    A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed
    Figure Legend Snippet: A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

    Techniques Used: Transduction, Control, Irradiation, Isolation, Knock-In, Incubation, Immunofluorescence, Western Blot, Single Cell Gel Electrophoresis, Software

    Related Articles

    Over Expression:

    Article Title: Declining activity of serum response factor in aging aorta in relation to aneurysm progression
    Article Snippet: .. For MYOCD overexpression, a replication-deficient adenovirus (Ad-h-MYOCD or MYOCD, ADV-216227) was acquired from Vector Biolabs, with Ad-CMV-Null (#1300) used as the control. ..

    Article Title: Declining activity of serum response factor in aging aorta in relation to aneurysm progression.
    Article Snippet: .. For myocardin overexpression, a replication-deficient adenovirus (Ad-hMYOCD or MYOCD, ADV-216227) was acquired from Vector Biolabs, with Ad-CMV-Null (#1300) used as the control. ..

    Control:

    Article Title: Declining activity of serum response factor in aging aorta in relation to aneurysm progression
    Article Snippet: .. For MYOCD overexpression, a replication-deficient adenovirus (Ad-h-MYOCD or MYOCD, ADV-216227) was acquired from Vector Biolabs, with Ad-CMV-Null (#1300) used as the control. ..

    Article Title: Increased fatty acid delivery by tumor endothelium promotes metastatic outgrowth.
    Article Snippet: Cells were infected with 107 PFU ml-1 of Ad-CMV-iCre (Vector Biolabs, #1045) for 16-48 hours, as indicated. .. Ad-CMV-b-Gal (Vector Biolabs #1080) or Ad-CMV-Null (Vector Biolabs, #1300) were used as control vectors as indicated. ..

    Article Title: Increased fatty acid delivery by tumor endothelium promotes metastatic outgrowth
    Article Snippet: Cells were infected with 1 × 10 7 PFU/mL of Ad-CMV-iCre (Vector Biolabs, 1045) for 16–48 hours, as indicated. .. Ad-CMV-b-Gal (Vector Biolabs, 1080) and Ad-CMV-Null (Vector Biolabs, 1300) were used as control vectors as indicated. ..

    Article Title: Myocardin related transcription factor and galectin-3 drive lipid accumulation in human blood vessels.
    Article Snippet: Objective: Diabetes and hypertension are important risk factors for vascular disease, including atherosclerosis.. A driving factor in this process is lipid accumulation in smooth muscle cells of the vascular wall.. The glucoseand mechano-sensitive transcriptional coactivator, myocardin-related transcription factor A (MRTF-A/MKL1) can promote lipid accumulation in cultured human smooth muscle cells and contribute to the formation of smooth muscle-derived foam cells.

    Article Title: Regulation of fatty acid delivery to metastases by tumor endothelium
    Article Snippet: Cells were infected with 10 7 PFU ml -1 of Ad-CMV-iCre (Vector Biolabs, #1045) for 16-48 hours, as indicated. .. Ad-CMV-b-Gal (Vector Biolabs #1080) or Ad-CMV-Null (Vector Biolabs #1300) were used as control vectors as indicated. ..

    Article Title: Declining activity of serum response factor in aging aorta in relation to aneurysm progression.
    Article Snippet: .. For myocardin overexpression, a replication-deficient adenovirus (Ad-hMYOCD or MYOCD, ADV-216227) was acquired from Vector Biolabs, with Ad-CMV-Null (#1300) used as the control. ..

    Transduction:

    Article Title: Extracellular matrix regulates lineage plasticity in prostate cancer through YAP/TEAD
    Article Snippet: .. For adenoviral transduction, 1 μL of Ad-CMV-Null (Vector Biolabs, 1300) or 1 μL of Ad-CMV-iCre (Vector Biolabs, 1045) was used to infect 5 × 104 cells. ..



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    Image Search Results


    A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

    Journal: bioRxiv

    Article Title: ATGL-catalyzed lipid catabolism promotes DNA repair

    doi: 10.64898/2026.04.03.716381

    Figure Lengend Snippet: A) AML12 cells transduced with ATGL or GFP control adenoviruses were treated with 30 μM etoposide for the indicated times and assayed for γH2Ax. B) Quantification of A. *p<0.05, **p<0.01. C) AML12 cells transduced with ATGL or GFP control adenoviruses were irradiated (10 Gy) and harvested at the indicated times post-IR. D) Quantification of C. **p<0.01. E) Primary MEFs were isolated from WT or ATGL knock-in (AKI) mice, followed by etoposide incubation for 6 hours. Immunofluorescence was performed for γH2Ax, and cells were imaged using the Agilent Gen5 Cytation. Representative γH2Ax images and foci distribution are shown. F) Cells were treated with DGAT inhibitors for 24 hours, followed by ATGL adenoviral transduction, followed by 24 hours of etoposide treatment. Western blotting for γH2Ax, β-actin, and ATGL. G) AML12 cells were treated as in F. The Comet assay was performed on cells after 24 hours of etoposide. Comets were quantified with OpenComet software. A minimum of 180 comets were quantified per condition. Statistics: one-way ANOVA with Tukey’s post hoc test. ****p<0.0001. H) γH2Ax results and quantification from tissues harvested from E2A Cre-ATGL (AKI) mice irradiated with 7.5 Gy + 4-hour recovery. Mice were of mixed sexes, n=4 mice. P-values from one-way ANOVA are displayed

    Article Snippet: For adenoviral transductions, ATGL (Lot: 20160819, mouse ATGL) was obtained from VectorBiolabs, TrackGFP (adGFP) was obtained from UNC Gene Therapy Center, and Null adenovirus was obtained from VectorBiolabs (#1300).

    Techniques: Transduction, Control, Irradiation, Isolation, Knock-In, Incubation, Immunofluorescence, Western Blot, Single Cell Gel Electrophoresis, Software